166,034 results

1. pUC-L3L2

   Plasmid

   #114019

   Purpose

   Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning

   Depositor

   Christopher Grefen

   Insert

   ccdB gene, Chloramphenicol resistance gene
   

   Use

   Gateway

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLJM1 flag-cFLIP

   Plasmid

   #211529

   Purpose

   Expresses flag-tagged cFLIP (CFLAR)

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   cFLIP (CFLAR Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC-L1L4

   Plasmid

   #114018

   Purpose

   Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning

   Depositor

   Christopher Grefen

   Insert

   ccdB gene, Chloramphenicol resistance gene
   

   Use

   Gateway

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGPI-SceI

   Plasmid

   #32060

   Purpose

   Construction of unmarked gene deletions. Suicide cloning vector designed to introduce a targeted I-SceI restriction site in the genome of Burkholderia

   Depositor

   Miguel Valvano

   Article

   Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

   Type

   Empty backbone

   Use

   Mutagenesis plasmid

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-TID-HA3-KanMX6

   Plasmid

   #227980

   Purpose

   To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, KanMX6 marker

   Depositor

   Joseph Reese

   Article

   Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

   Insert

   TurboID
   

   Tags

   HA tag

   Expression

   Yeast

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLminP_Luc2P_RE48

   Plasmid

   #90387

   Purpose

   IRF3/IRF7 - viral response gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Promoter

   IRF3/IRF7

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST47-ARL3-GFP

   Plasmid

   #67397

   Purpose

   Mammalian expression of hArl3 with C-term GFP tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL3 (ARL3 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

8. TFORF0195

   Plasmid

   #142554

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RFX7 (RFX7 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBbB2k-GFP

   Plasmid

   #35345

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Tet

   Available Since

   Feb. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ch-gelsolN

    Plasmid

    #37262

    Depositor

    Ikuo Wada

    Article

    Nagaya et al J Cell Biol. 2008 Jan 14;180(1):129-43.

    Insert

    gelsolin (GSN Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    deleted amino acids 380-731

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pLP_mod_mNeonGreen11_P2A_puroR_CRE-GFP

    Plasmid

    #229227

    Purpose

    Modular BxB1 landing pad vector containing C-term mNeonGreen11 tag, puroR marker, and a cAMP GFP reporter.

    Depositor

    Willow Coyote-Maestas

    Article

    Howard et al Cell. 2025 Feb 6;188(3):671-687.e20. doi

    Type

    Empty backbone

    Tags

    mNeonGreen11

    Expression

    Mammalian

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-sgCDKN1B#2 BFP

    Plasmid

    #60905

    Purpose

    A lentiviral plasmid that encodes both a sgRNA targeting CDKN1B and BFP

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tol2-CAG::Nucbow

    Plasmid

    #158992

    Purpose

    Multicolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)

    Depositor

    Jean Livet

    Article

    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

    Insert

    Nucbow
    

    Tags

    All FPs are fused to H2B

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMU-ACTFr

    Plasmid

    #61191

    Purpose

    Fluorescent reporter for F-actin AtFim-ABD2-mCherry expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    AtFimbrin1-ABD
    

    Tags

    mCherry

    Expression

    Plant

    Promoter

    AtUBQ10

    Available Since

    Feb. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. FR_HNF1B

    Plasmid

    #31101

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

    Insert

    HNF1B (HNF1B Human)
    

    Use

    Flp/frt

    Tags

    myc

    Expression

    Mammalian

    Mutation

    wild type

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB_iNEUROD1_P2A_GFP_Puro

    Plasmid

    #168803

    Purpose

    PiggyBac transposon construct with doxycycline inducible NEUROD1 + GFP expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    NEUROD1 (NEUROD1 Human, Synthetic)
    

    Use

    Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPPI4-Strep-SNAP-PD-1

    Plasmid

    #223569

    Purpose

    Protein purification of human PD-1 with Twin-Strep and SNAP tags from mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

    Insert

    PD-1 (PDCD1 Human)
    

    Tags

    Twin-Strep, SNAP

    Expression

    Mammalian

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDAI-SceI-SacB

    Plasmid

    #113635

    Purpose

    Broad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacB

    Depositor

    Miguel Valvano

    Article

    Aubert et al Methods Mol Biol. 2014;1197:311-27. doi:

    Insert

    sacB
    

    Expression

    Bacterial

    Mutation

    Please see Depositor Comments

    Promoter

    sacB promoter

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. FU-mILa-CRW

    Plasmid

    #84556

    Purpose

    lentiviral expression of mouse IL1a and mRFP

    Depositor

    Li Xin

    Article

    Zhang et al Mol Cell. 2016 Sep 15;63(6):976-89. doi:

    Insert

    IL1a (Il1a Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJRA104

    Plasmid

    #209337

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal HaloTag fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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