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Showing: 1961 - 2000 of 151085 results

  6. Desmoplakin II no force control (F40-based)

    Plasmid
    #118716
    Purpose
    The no force control for the F40-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)-F40-mCherry] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry
    Expression
    Mammalian
    Mutation
    truncation after aa1353
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118716-plasmid-map-sequence-id-230464

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  19. pLPC-C-SF-TAP-DEST-TLK1- M566T

    Plasmid
    #218831
    Purpose
    Expresses C-terminal Strep-FLAG-tagged M566T human TLK1 variant 5 in a retrovirus vector.
    Depositor
    Travis Stracker
    Insert
    TLK1 M566T (TLK1 Human)
    Use
    Tags
    FLAG-STREP
    Expression
    Mammalian
    Mutation
    M566T
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218831-plasmid-map-sequence-id-436656

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  20. pLPC-C-SF-TAP-DEST-TLK1- Q479E

    Plasmid
    #218830
    Purpose
    Expresses C-terminal Strep-FLAG-tagged Q479E human TLK1 variant 5 in a retrovirus vector.
    Depositor
    Travis Stracker
    Insert
    TLK1 Q479E (TLK1 Human)
    Use
    Tags
    FLAG-STREP
    Expression
    Mammalian
    Mutation
    Q479E
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218830-plasmid-map-sequence-id-436654

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  24. pDEST17-His10-Smt3-ccdB-SNAP

    Plasmid
    #166148
    Purpose
    Gateway destination vector for generation of N-terminal His10-Smt3 and C-terminal SNAP tagged proteins in E. coli.
    Depositor
    Daniel Jarosz
    Article
    Itamar Harel et al BioRxiv
    Type
    Empty backbone
    Use
    Tags
    His10-Smt3 and SNAP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    166148-plasmid-map-sequence-id-328896

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  29. pDEST-Spe4-Kozak-emiRFP703-RfA

    Plasmid
    #186376
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter emiRFP703 and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    186376-plasmid-map-sequence-id-398283

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  31. pDEST-Myc-FBW7 Delta WD40 (-2 to 7)

    Plasmid
    #197454
    Purpose
    The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 propellars 2 to 7 are deleted. Used for mammalian overexpression and detection by western blotting.
    Depositor
    Omar Khan
    Article
    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:
    Insert
    FBW7 Delta-WD40 propeller (FBXW7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    FBW& dimerization domain deleted.
    Promoter
    CMV
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    197454-plasmid-map-sequence-id-394916

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  33. pDEST-Spe4-RfA-mVenus-stop

    Plasmid
    #186394
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mVenus into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    186394-plasmid-map-sequence-id-392701

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  34. pDEST-Spe4-Kozak-miRFP713-RfA

    Plasmid
    #186381
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter miRFP713 and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    186381-plasmid-map-sequence-id-392698

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  35. pDEST-Spe4-Kozak-Halotag-RfA

    Plasmid
    #186377
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter Halo tag and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    186377-plasmid-map-sequence-id-392694

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  36. pcDNA3.2-V5-DEST-hSFRP1(aa53-166)

    Plasmid
    #189544
    Purpose
    Protein expression of SFRP1 in mammalian cells. Contains only Frizzled cysteine rich domain (CRD)--amino acids 53-166.
    Depositor
    Haian Fu
    Insert
    truncated secreted frizzled related protein 1 (SFRP1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    contains aa53-166
    Promoter
    Available since
    April 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    189544-plasmid-map-sequence-id-390137

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  37. pDEST27-GST-hSFRP1(aa181-314)

    Plasmid
    #189548
    Purpose
    Protein expression of SFRP1 in mammalian cells. Contains only Netrin (NTR) domain--amino acids 181-314.
    Depositor
    Haian Fu
    Insert
    truncated secreted frizzled related protein 1 (SFRP1 Human)
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    contains aa181-314
    Promoter
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    189548-plasmid-map-sequence-id-374132

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  38. pDEST27-GST-hSFRP1(aa53-169)

    Plasmid
    #189545
    Purpose
    Protein expression of SFRP1 in mammalian cells. Contains only Frizzled cysteine rich domain (CRD)--amino acids 53-169.
    Depositor
    Haian Fu
    Insert
    truncated secreted frizzled related protein 1 (SFRP1 Human)
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    contains aa53-169
    Promoter
    Available since
    Oct. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    189545-plasmid-map-sequence-id-374145

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Showing: 1961 - 2000 of 151085 results