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LaM8-GG15-irFP
Plasmid#159591PurposeOpto-NanobodyDepositorInsertLaM8 with AsLOV2 at GG15
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_ScPH1-S
Plasmid#131193PurposeExpresses ScPH1-S (Synechosystis PCC6803) preceded by restriction sitesDepositorInsertScPH1-S
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMN-327
Plasmid#195452PurposeLentiviral Integratable mCherry Reporter for synZiFTR ZF10DepositorInsert8X ZF10 BS - minTK - mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH29_32 series plasmid
Plasmid#120901PurposeAlso known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated deviceDepositorInsertsEc-TTL-P109
sRNA-A targeting sequence
RBS TIR=974 of GFPop1 gene
GFPop1
RBS TIR=6474 of ECF32mut gene
ECF32mut
T7 terminator
resource competitor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CibN-dCas9
Plasmid#60551PurposeExpresses CibN-dCas9 in mammalian cellsDepositorInsertCibN-dCas9
UseCRISPRTagsFLAG and SV40 NLSExpressionMammalianMutationinactivating Cas9 mutations D10A and H840APromoterCMVAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMN-294
Plasmid#195453PurposeLentiviral Integratable mCherry Reporter for synZiFTR ZF1DepositorInsert8X ZF1 BS - ybTATA - mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-NPM NESM
Plasmid#13282DepositorInsertNPM (NPM1 Human)
UseTagsFlagExpressionMammalianMutationL100A, L102APromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-Otx2shRNA1
Plasmid#73979PurposeOtx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.DepositorInsertshRNA that targets the mouse Otx2 gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
XW52
Plasmid#65838PurposeXW52 Pmig-13-Czipper-QF-AD-NLS-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional methodDepositorInsertCzipper-QF-AD-NLS
UseTagsmCherryExpressionWormMutationPromoterPmig-13Available sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMK375 (Hygro-P2A-Stag-3FLAG)
Plasmid#121186PurposeN-terminal taggingDepositorInsertHygro-P2A-Stag-3FLAG
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0553
Plasmid#128989PurposeLEU2 integration vector of pZF(3BS)-mRUBY2DepositorInsertLeu2 5' homology-pZiF promoter-mRuby2-tADH1-Con1-LEU2-Leu2 3' homology
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
tCRMod-GGSGGS7
Plasmid#111761PurposeExpresses soluble Clover-mRuby2 tension sensor module with 7-repeat GGSGGS extensible domainDepositorInsertClover-GGSGGS7-mRuby2 fusion
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-Cerulean-SKL
Plasmid#194048PurposeTransient expression of Cerulean-SKL in plant cell (Peroxisome)DepositorInsertCerulean
UseTagsSer-Lsy-LeuExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMN-319
Plasmid#195447PurposeLentiviral Integratable mCherry Reporter for synZiFTR ZF1DepositorInsert8X ZF1 BS - minCMV - mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-NPM NLS1D
Plasmid#13284DepositorInsertNPM (NPM1 Human)
UseTagsFlagExpressionMammalianMutationDeletion of aa134-aa142PromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJB01
Plasmid#111957PurposeExpresses His10-RFP-Halo; construct generated for pull-down and localization experimentsDepositorInsertRFP
UseTagsHaloTagExpressionBacterialMutationPromoterT7Available sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
tCRMod-GGSGGS5
Plasmid#111760PurposeExpresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domainDepositorInsertClover-GGSGGS5-mRuby2 fusion
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAd-Track SIRT1
Plasmid#8440DepositorInsertSIRT1 (Sirt1 Mouse)
UseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
H2B-GFP-AsLOV2-NES27 (pDN158)
Plasmid#72659PurposeConstitutively nuclear variant of a very strong, photocaged NES (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear exportDepositorInsertH2B-GFP-AsLOV2-NES27
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMN-321
Plasmid#195449PurposeLentiviral Integratable mCherry Reporter for synZiFTR ZF10DepositorInsert8X ZF10 BS - minCMV - mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15bLCFPT7
Plasmid#53545PurposeActivation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistantDepositorInsertT7 RNAP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
ptagBFP-LacI
Plasmid#103839PurposeFlourescent marker for lacO arraysDepositorInserttagBFP-LacI
UseTagsExpressionMammalianMutationLacI: C19T (silent, L7L), T264C (silent, A88A), C…PromoterSV2Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAFZifPZ431
Plasmid#185849PurposeExpressing artificial trans-activator in Saccharomyces cerevisiaeDepositorInsertPHAC1>ZifPZ43>MED3>PABF1
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-NPM NLS1/2D
Plasmid#13287DepositorInsertNPM (NPM1 Human)
UseTagsFlagExpressionMammalianMutationDeletion of aa134-aa142, aa150-aa155PromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEM705 Gst (Cys)
Plasmid#109376PurposeGsX chimera with Gαt C-terminus (Pertussis toxin sensitive)DepositorInsertGst chimera
UseTagsNoneExpressionMammalianMutationCys at position 391 (Pertussis Toxin Sensitive)PromoterCAGAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-CibN
Plasmid#60552PurposeExpresses dCas9-CibN in mammalian cellsDepositorInsertdCas9-CibN
UseCRISPRTagsFLAG and SV40 NLSExpressionMammalianMutationinactivating Cas9 mutations D10A and H840APromoterCMVAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-NPM NESM-NLS1D
Plasmid#13288DepositorInsertNPM (NPM1 Human)
UseTagsFlagExpressionMammalianMutationL100A, L102A, deletion of aa134-aa142PromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pNUT3
Plasmid#175744PurposeSingle-component photo-inducible (CRY2-based) nucleolus-targeting tool 3DepositorInsertmCherry-CRY2PHR-NoLS (human nuclear factor-ÎşB inducing kinase)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
VV246: CoChR(C108S)-GCaMP6f-ER in fck
Plasmid#58521PurposeExpresses CoChR(C108S) fused to GCaMP6f in mammalian cellsDepositorInsertCoChR(C108S)-GCaMP6f-ER
UseLentiviralTagsGCaMP6fExpressionMammalianMutationchanged Cysteine 108 to SerinePromotera-CamKIIAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAurora
Plasmid#190158PurposeRegulates bacterial expression of target gene in blue-light-activated manner. Based on Nakamurella multipartita PAL.DepositorTypeEmpty backboneUseTagsHis6 tagExpressionBacterialMutationPromoterAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCrepusculo
Plasmid#190156PurposeRegulates bacterial expression of target gene in blue-light-repressed manner. Based on Nakamurella multipartita PAL.DepositorTypeEmpty backboneUseTagsHis6 tagExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Double UP sfGFP to mScarlet
Plasmid#120261PurposeNatively produces sfGFP, in the presence of Cre will be recombined to produce mScarlet.DepositorInsertsfGFP/mScarletP2A-mcs
UseTagsExpressionMammalianMutationRemoved Not1 site in mScarletPromoterCAGAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI-HA-mAVO3 (pRL62-3)
Plasmid#39210DepositorInsertmAVO3 (Rictor Mouse)
UseTagsHAExpressionMammalianMutationG5S and I15VPromoterCMVAvailable sinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
p416GPD-PYK1
Plasmid#34604DepositorInsertPYK1
UseTagsExpressionYeastMutationPromoterGPDAvailable sinceJan. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
Double UP mClover3 to mScarlet
Plasmid#120262PurposeNatively produces mClover3, in the presence of Cre will be recombined to produce mScarlet.DepositorInsertmClover3/mScarletP2A-mcs
UseTagsExpressionMammalianMutationRemoved Not1 site in mScarletPromoterCAGAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-NPM NESD
Plasmid#13283DepositorInsertNPM (NPM1 Human)
UseTagsFlagExpressionMammalianMutationDeletion of aa94-aa102PromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTn7·xylS
Plasmid#122591PurposePlasmid for genomic integraction of the xylS regulator into the Tn7 insertion siteDepositorInsertxylS
UseSynthetic Biology; Tn7 genomic integrationTagsExpressionBacterialMutationPromoterPmAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
LaM8-GG15
Plasmid#159593PurposeOpto-NanobodyDepositorInsertLaM8 with AsLOV2 at GG15
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB80-iLID-mCherry-RAB5
Plasmid#174624PurposeOptogenetic coupling to early endosomes (RAB5) via iLIDDepositorInsertiLID-mCherry-RAB5 (RAB5A Human, Synthetic)
UseTagsiLID-mCherryExpressionMammalianMutationmCherry: Met1Del; RAB5: Met1DelPromoterChicken beta-actinAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only
