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Flag-HA-USP5

Plasmid

#22590

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP5 (USP5 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCasAb-apr

Plasmid

#121998

Purpose

Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii

Depositor

Quanjiang Ji

Publication

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAct-Cas9

Plasmid

#62209

Purpose

Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.

Depositor

Simon Bullock

Publication

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

cas9

Expression

Insect

Promoter

act5C

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pENTR-ERKKTRClover

Plasmid

#59138

Purpose

ORF encoding ERK KTR mClover flanked by Gateway sequences

Depositor

Markus Covert

Publication

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

ERK Kinase Translocation Reporter (MAPK1 Human, Mouse)

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pDONR223_Luciferase

Plasmid

#25894

Purpose

Gateway entry vector containing Luciferase.

Depositor

David Root

Publication

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

Luciferase

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pMOD_B2403

Plasmid

#91071

Purpose

Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with ribozyme spacers , Terminator: 35S

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

SapI ccdb cassette for cloning multiple gRNA spacers with ribozyme spacers

Use

CRISPR

Promoter

CmYLCV

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pmGFP-P2A-K(AAA)20-P2A-RFP

Plasmid

#105688

Purpose

Double fluorescent stalling reporter K(AAA)20

Depositor

Ramanujan Hegde

Publication

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

P2A-3xFLAG-VHPbeta
K(AAA)20
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pEF-ENTR A (696-6)

Plasmid

#17427

Purpose

Gateway entry vector with EF1-a promoter, V5-His C-term, frame A

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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v3em-Cterm-PE2max-∆RNaseH-dualU6

Plasmid

#198735

Purpose

AAV genome encoding C-terminal PE2max and U6 expression cassettes

Depositor

David Liu

Publication

Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

Insert

NpuC-CtermPE2max∆RNaseH

Use

AAV

Promoter

Cbh

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pZR071_SFFV-dCas9-mCherry-KRAB

Plasmid

#180264

Purpose

Lentiviral expression vector for dCas9-KRAB in mammalian cells

Depositor

Alexander Marson

Publication

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

KRAB and mCherry

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_SV40_Large_T_Antigen-P2A-Hygro_Barcode

Plasmid

#170255

Purpose

Barcoded lentiviral vector to express SV40 Large T Antigen in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Publication

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

SV40 Large T Antigen

Use

Lentiviral

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pGP-AAV-syn-jGCaMP8f-WPRE

Plasmid

#162376

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Publication

Zhang et al Janelia Research Campus, Online Resource

Has service

AAV1, AAV5, AAV9, and AAV Retrograde

Insert

jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Q315L

Promoter

Synapsin

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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p6XHis_NLS-SaCas9

Plasmid

#101086

Purpose

Express SaCas9 in bacteria with a 6xHis tag for purification

Depositor

Rick Tarleton

Publication

Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

Insert

CRISPR-associated protein Cas9/Csn1

Use

CRISPR

Tags

TrxA-6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFREE

Plasmid

#92050

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Publication

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Expression

Bacterial

Promoter

pRhamBAD and ptet

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBK-VQR-BE3

Plasmid

#85171

Purpose

Expresses VQR-BE3 in mammalian cells

Depositor

David Liu

Publication

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VQR-BE3

Expression

Mammalian

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDESTmycDDX17

Plasmid

#19876

Depositor

Thomas Tuschl

Publication

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17 Human)

Tags

MYC

Expression

Mammalian

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pAAV-hSyn-FLEX-TeLC-P2A-dTomato

Plasmid

#159102

Purpose

Cre-dependent tetanus toxin light chain construct with nuclear-localized dTomato fluorophore for conditional silencing of neurons.

Depositor

Sandeep Datta

Publication

Pashkovski et al Nature. 2020 Jul;583(7815):253-258. doi:

Insert

TeLC-P2A-NLS-dTomato

Use

AAV

Promoter

hSynapsin

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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Nb-gp41-GFP (MoonTag-Nb-GFP)

Plasmid

#128602

Purpose

Expresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

Depositor

Marvin Tanenbaum

Publication

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

Nanobody_gp41

Use

Lentiviral

Tags

GB1 and sfGFP

Promoter

SFFV

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-CRBN-pGK-HYG

Plasmid

#107380

Purpose

Mammalian expression of FLAG-CRBN

Depositor

William Kaelin

Publication

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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psfTn5

Plasmid

#79107

Purpose

bacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutations

Depositor

Sten Linnarsson

Insert

transposase 5

Tags

His

Expression

Bacterial

Mutation

E54K and L372P

Available since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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