We narrowed to 18,496 results for: Met

1. pSNA

   Plasmid

   #135192

   Purpose

   Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassette

   Depositor

   Hongqing Fang

   Article

   Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

   Insert

   I-SceI lamba Red
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   None

   Promoter

   araBAD

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1(+)-NEMOm

   Plasmid

   #189930

   Purpose

   Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

   Depositor

   Yubin Zhou

   Article

   Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

   Insert

   NEMOs
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas9 plasmid

   Plasmid

   #100091

   Purpose

   dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3XFlag-NLS-dCas9-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. rAAV2/MG1.1

   Plasmid

   #184540

   Purpose

   AAV packaging plasmid, expressing Rep2 and AAV-MG1.1 capsid

   Depositor

   Minmin Luo

   Article

   Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

   Insert

   AAV-MG1.1 capsid
   

   Use

   AAV; Helper vector for packaging

   Mutation

   changes on AAV9 capsid: K449R, A587L, Q588M; inse…

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. dCas9-10xSunTag-P2A-tagBFP

   Plasmid

   #174141

   Purpose

   Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.

   Depositor

   Albert Jeltsch

   Article

   Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

   Insert

   dCas9, 10xSunTag, tagBFP
   

   Tags

   HA and tagBFP

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hChATp-tdTomato

   Plasmid

   #197564

   Purpose

   Expresses tdTomato under a human choline acetyltransferase (hChAT) promoter fragment

   Depositor

   Casey Maguire

   Article

   Santoscoy et al Mol Ther Methods Clin Dev. 2023 May 9;29

   Insert

   tdTomato
   

   Use

   AAV

   Promoter

   human ChAT promoter

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1(+)-NEMOs

   Plasmid

   #189931

   Purpose

   Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

   Depositor

   Yubin Zhou

   Article

   Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

   Insert

   NEMOs
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody

   Plasmid

   #138133

   Purpose

   Expresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   EGFP-anti-rabbit-IgG(Fc)-nanobody
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDx_mCherry-SYFP2

   Plasmid

   #189759

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mCherry-SYFP2 fusion protein for FRET analysis

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mCherry-SYFP2
   

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Promoter

   CMV & Rha

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3.1(+)-NEMOc

    Plasmid

    #189933

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOc
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR

    Plasmid

    #154003

    Purpose

    Vector expressed CasRx and sgRNA_LACZ for AAV package

    Depositor

    Hui Yang

    Article

    He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

    Insert

    CasRx
    

    Use

    AAV and CRISPR

    Promoter

    EFS

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEMHE-mCherry-mTrim21

    Plasmid

    #105522

    Purpose

    T7 promotor drives in vitro transcription of mCherry-tagged wildtype mouse TRIM21 mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mCherry

    Promoter

    T7

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS_cAMPFIRE-M

    Plasmid

    #182284

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-M (cAMP sensor)
    

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. plenti hSyn PKM1

    Plasmid

    #220920

    Purpose

    Neuronal specific expression of PKM1

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    PKM1 (PKM Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Snap-Omp25

    Plasmid

    #69599

    Purpose

    Snap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondria

    Depositor

    David Sabatini

    Article

    Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

    Insert

    Snap-Omp25
    

    Expression

    Mammalian

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSIQ-EGFP

    Plasmid

    #216239

    Purpose

    All-in One IQ transgene switch for EGFP expression

    Depositor

    Hyunju Ro

    Article

    Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJW31

    Plasmid

    #136423

    Purpose

    Contains genes of Type I-C CRISPR-Cas system for inducible expression and chromosomal integration at attTn7

    Depositor

    Joseph Bondy-Denomy

    Article

    Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

    Inserts

    Cas5
    Cas8
    Cas7
    Cas3
    Cas3
    

    Use

    CRISPR

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOsTIR1w/oGFP

    Plasmid

    #102883

    Purpose

    Expresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.

    Depositor

    Matthias Heinemann

    Article

    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

    Insert

    OsTIR1
    

    Tags

    3x myc

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiCRISPR v2 hBAX

    Plasmid

    #129580

    Purpose

    CRISPR deletion of human BAX

    Depositor

    Stephen Tait

    Article

    Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

    Insert

    gRNA vs human BAX (BAX Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWZL Blast DN E-cadherin

    Plasmid

    #18800

    Depositor

    Bob Weinberg

    Article

    Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

    Insert

    Dom neg E-cadherin (Cdh1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    truncated form of E-cadherin (dominant negative) …

    Available Since

    July 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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