8,720 results
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Plasmid#70148Purposea targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyADepositorInsert5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh
UseMouse Targeting and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZR071_SFFV-dCas9-mCherry-KRAB
Plasmid#180264PurposeLentiviral expression vector for dCas9-KRAB in mammalian cellsDepositorInsertdCas9
UseCRISPR and LentiviralTagsKRAB and mCherryExpressionMammalianMutationNuclease dead Cas9PromoterSFFVAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-DsRed
Plasmid#13769PurposeCre recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.DepositorInsertDsRed2
UseCre/Lox; Flp/frtTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9
Plasmid#47106PurposeExpresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cellsDepositorInsertdCas9
UseCRISPRTagsFlag and SV40 NLSExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-ER alpha
Plasmid#28230PurposeMammalian expression of Estrogen Receptor alpha fused to EGFPDepositorInsertEstrogen Receptor alpha (ESR1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pYPQ202
Plasmid#86198PurposeMultisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromiycin resistance markerDepositorTypeEmpty backboneUseGateway compatible attr1-attr2 destination vectorTagsExpressionPlantMutationPromoterArabidopsis ubiquitin 10 (AtUbi10)Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBa-MARK2-eGFP
Plasmid#66706Purposemammalian expression vector for MARK2 with a C-terminal eGFPDepositorInsertMARK2 (MARK2 Human)
UseTagseGFPExpressionMammalianMutationPromoterchicken beta actinAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGWB402
Plasmid#74796PurposeGateway cloning compatible binary vector for expression of gene by CaMV35S promoter.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSL1142 (pSPIN, pBBR1 backbone)
Plasmid#160730PurposeSingle-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
spNW25
Plasmid#173484PurposeExpresses spNW25 in Bl21DepositorInsertspNW25
UseTagsHistagExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21 (ORF-EG)
Plasmid#46948PurposeInducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interestDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLM494
Plasmid#100539PurposeCEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.DepositorInsertsGGPP synthase
phytoene desaturase
phytoene-beta carotene synthase
HMG CoA reductase
UseCre/Lox and Synthetic BiologyTagsExpressionYeastMutationPromoterPGK1, TDH3, TIP1, and ZEO1Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Olig001
Plasmid#170716PurposeOlig001 encodes a novel AAV capsid that exhibits preferential oligodendrocyte tropism in the CNSDepositorInsertOlig001 capsid gene / rep 2
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
CRE recombinase
Plasmid#62730PurposeExpresses Cre recombinase protein in bacterial cellsDepositorInsertCre recombinase
UseCre/LoxTagsHis tag and NLSExpressionBacterialMutationCodon optimazed for e.coliPromoterT7Available sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
tau/pET29b
Plasmid#16316DepositorInserttau (MAPT Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
PB_tre_dCas9_KRAB
Plasmid#126030PurposePiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusionDepositorInsertsdCas9 fusion with KRAB
Hygromycin resistance
UseCRISPR; PiggybacTagsExpressionMammalianMutationD10A, H840A (catalytically inactive Cas9)PromoterEF1 alpha and TREAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2 hygro
Plasmid#98291PurposeVariant of lentiCRISPRv2 that confers hygromycin resistanceDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSI-Check2-hRluc-NFkB-firefly
Plasmid#106979PurposeLuciferase reporter with internal control for NFkB signal.DepositorInsertNFKB recognition sites (NFKB1 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-pBAD
Plasmid#54572PurposeLocalization: Protein Expression Vector , Excitation: 399, Emission: 456DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis, T7, and mTagBFP2ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV-TetO-hNGN2-eGFP-Puro
Plasmid#79823PurposeExpresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.DepositorInserthNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)
UseLentiviralTagsExpressionMutationPromoterTetOAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only