We narrowed to 3,430 results for: Agt

1. pOC2 GFP-Actb KI

   Plasmid

   #183429

   Purpose

   CreOFF knock-in for GFP-beta-actin (amino acid position: before start codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and GFP donor
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1046E=dgRNAs[traB,bTub]

   Plasmid

   #149425

   Purpose

   The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes.

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

   Insert

   U6.3-gRNAs[TraB, bTub]
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6.3

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX458_sgRNA_Ago1_6

   Plasmid

   #172471

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1.

   Depositor

   Constance Ciaudo

   Article

   Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

   Insert

   sgAgo1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. TLCV2-sgHPDL #3

   Plasmid

   #174165

   Purpose

   knock out HPDL in human cell lines

   Depositor

   Michael Pacold

   Article

   Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

   Insert

   sgRNA against HPDL
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. TLCV2-sgHPDL #9

   Plasmid

   #174166

   Purpose

   knock out HPDL in human cell lines

   Depositor

   Michael Pacold

   Article

   Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

   Insert

   sgRNA against HPDL
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Bax gRNA#1

   Plasmid

   #171827

   Purpose

   Cas9-mediated knockout of Bax in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bax (Bax Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJAID5WY

   Plasmid

   #165050

   Purpose

   Integrative expression of SOD1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>SOD1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   OsTIR1 is codon-optimised for expression in S. ce…

   Promoter

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBbdCas9S_Psyn-sgRNAftsI

   Plasmid

   #149649

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAftsI
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBbdCas9S_P0526-sgRNAftsI

   Plasmid

   #149650

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAftsI
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, P0526

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBSV2H_Psyn-sgRNAftsI

    Plasmid

    #149563

    Purpose

    ftsI gRNA expression in B. burgdorferi

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    sgRNAftsI
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX300A-G+Nanog

    Plasmid

    #140280

    Purpose

    CRISPR/Cas9 plasmid encoding Cas9 and sgRNA against gBait and Nanog locus

    Depositor

    Yusuke Miyanari

    Article

    Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBH

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLY132

    Plasmid

    #130960

    Purpose

    Engineered sgRNA-LEB3 generator including Anderson promoter J23100 for golden gate assembly

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEB3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Anderson promoter J23100

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV2-U6(gDmap1 v1)-PGK-Puro-BFP

    Plasmid

    #117140

    Purpose

    Express gRNA vs Dmap1

    Depositor

    Kosuke Yusa

    Insert

    gDmap1 v1 (Dmap1 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLENTICRISPR sgNFS1

    Plasmid

    #102979

    Purpose

    Cutting NFS1 genomic locus

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    sgNFS1 (NFS1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRRNA-G11-R0

    Plasmid

    #101805

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    David Bikard, Sven van Teeffelen

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX458_ZNF511_1

    Plasmid

    #104044

    Purpose

    Encodes gRNA for 3' target of human ZNF511 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZNF511 gRNA (ZNF511 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PX458_ZNF511_2

    Plasmid

    #104045

    Purpose

    Encodes gRNA for 3' target of human ZNF511 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZNF511 gRNA (ZNF511 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRRNA-G0-R11

    Plasmid

    #101808

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    David Bikard, Sven van Teeffelen

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRRNA-G14-R0

    Plasmid

    #101806

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    David Bikard, Sven van Teeffelen

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRRNA-G10-R0

    Plasmid

    #101804

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    David Bikard, Sven van Teeffelen

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTX203

    Plasmid

    #89267

    Purpose

    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsDEP1 gene, OsDEP1-gRNA02

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Insert

    OsDEP1-gRNA02
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Maize Ubiquitin1 promoter

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. LentiCRISPR-sgC17orf89-3

    Plasmid

    #86136

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting C17orf89

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    sgRNA 3 targeting C17orf89 (NDUFAF8 )
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PX458_RFXANK_2

    Plasmid

    #86365

    Purpose

    Encodes gRNA for 3' target of human RFXANK

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against RFXANK (RFXANK Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CAG-EGFP-RORbshRNA1

    Plasmid

    #73985

    Purpose

    RORb shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse RORb gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CAG-mCherry-Otx2shRNA2

    Plasmid

    #73980

    Purpose

    Otx2 shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse Otx2 gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CAG-mCherry-RORbshRNA1

    Plasmid

    #73983

    Purpose

    RORb shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse RORb gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. lentiCRISPR - C16orf80 sgRNA 1

    Plasmid

    #70651

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against C16orf80
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pLKO1-puro-U6-sgRNA-TS4

    Plasmid

    #69236

    Purpose

    U6 driven SpCas9 sgRNA expression for TS4 site

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS4 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pATN (pKD-G12)

    Plasmid

    #62691

    Purpose

    Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

    Insert

    amiR-eGFP419/amiR-eGFP123/tdTomato
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pATL (pKD-G10)

    Plasmid

    #62689

    Purpose

    Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

    Insert

    amiR-eGFP419/amiR-eGFP123
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge