We narrowed to 6,084 results for: nls

1. A3B-Cas9n-UGI-NLS

   Plasmid

   #198889

   Purpose

   Express full-length A3B BE3-based base editor

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. dCas9-NLS-gs10-cGR2176

   Plasmid

   #188258

   Purpose

   Plasmid ensures constitutive expression of the C-terminal fragment of split GR2 (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

   Depositor

   Roman Jerala

   Article

   Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

   Insert

   cGR2176
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. nGR2175-gs10-NLS-VPR

   Plasmid

   #188264

   Purpose

   Plasmid ensures constitutive expression of the N-terminal fragment of split GR2 (aa 1-175), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

   Depositor

   Roman Jerala

   Article

   Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

   Insert

   nGR2175
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. NLS-FUS-Citrine-PixE

   Plasmid

   #111506

   Purpose

   For the nuclear localization of the PixELL system

   Depositor

   Jared Toettcher

   Article

   Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

   Insert

   PixE (slr1693 )
   

   Use

   Lentiviral

   Tags

   Citrine, FUS, and NLS

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-NLS-FRB-VP16

   Plasmid

   #210515

   Purpose

   expresses NLS-FRB-VP16 component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   NLS-FRB-VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABE-puro-NLS-LSSmKate1

   Plasmid

   #31873

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   NLS-LSS-mKate1
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

   Promoter

   Available since

   Dec. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. 2xNLS-NLP-cMyc LbaCas12a

   Plasmid

   #182123

   Purpose

   pET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   2xNLS-NLP-cMyc LbaCas12a
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Thy1.2-NLS_HA_Dre-P2A-BFP

   Plasmid

   #51275

   Purpose

   Co-expression of the site-specific recombinase Dre and BFP under the control of the Thy1.2 expression cassette

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   NLS_HA_Dre-P2A-BFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Thy1.2

   Available since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPotef-LN22*-3xGNLS-cbx

   Plasmid

   #86780

   Purpose

   Expresses lambda N protein fused to triple Gfp coupled to an NLS sequence under the control of the constitutively active Potef promotor.

   Depositor

   Michael Feldbrügge

   Article

   Pohlmann et al Elife. 2015 May 18;4. doi: 10.7554/eLife

   Insert

   LN*
   

   Use

   Ustilago maydis expression

   Tags

   Gfp

   Expression

   Bacterial

   Mutation

   Promoter

   Potef

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Myog'-2A-mCherryNLS-PGK-Puro

    Plasmid

    #69548

    Purpose

    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Inserts

    2A-mCherry-NLS
    Myogenin 5' homology arm
    Myogenin 3' homology arm (Myog Mouse)
    

    Use

    Mouse Targeting

    Tags

    2x nuclear localization signal and PVT1-2A "…

    Expression

    Mutation

    Silent V205V mutation in myogenin gene to prevent…

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. dCas9-NLS-gs10-cDHFR174

    Plasmid

    #188256

    Purpose

    Plasmid ensures constitutive expression of the C-terminal fragment of split DHFR (aa 1-173), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    cDHFR174 (DHFR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. nDHFR173-gs10-NLS-VPR

    Plasmid

    #188262

    Purpose

    Plasmid ensures constitutive expression of the N-terminal fragment of split DHFR (aa 174-186), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    nDHFR173 (DHFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-alphaCaMKII-E2-Crimson-3XNLS-pA

    Plasmid

    #183803

    Purpose

    AAV vector to express nuclear localized E2-Crimson from CaMKIIa promoter

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

    Insert

    E2-Crimson-NLS
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-FNLSHiFi-P2A-Puro

    Plasmid

    #136902

    Purpose

    Lentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSHiFi
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Expression

    Mutation

    R691A

    Promoter

    EF1s

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC-U63-tgRNA-nlsBFP

    Plasmid

    #169029

    Purpose

    gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-mTagBFP-NLS marker.

    Depositor

    Chun Han

    Article

    Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid

    #69547

    Purpose

    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Inserts

    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    

    Use

    Mouse Targeting

    Tags

    2x nuclear localization signal and PVT1-2A "…

    Expression

    Mutation

    Silent V205V mutation in myogenin gene to prevent…

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPD355 VPR-FRB (NLS)

    Plasmid

    #138847

    Purpose

    Constitutive expression of VPR-FRB under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VPR-FRB
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTol2CG2-QUASM1:mScarletNLS-SV40pA

    Plasmid

    #155124

    Purpose

    Tol2 vector containing QUASM1 reporter element upstream of mScarlet-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QUASM1:mScarletNLS
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    QUASM1-E1b

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPHR-mCherry-NLS-VP16

    Plasmid

    #103821

    Purpose

    Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-mCherry-VP16 (CRY2 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    VP16: G126T (silent, G42G)

    Promoter

    CMV

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT2-7xTcf-NLS-ONL

    Plasmid

    #65713

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, ONL
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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