We narrowed to 11,881 results for: pcDNA

1. pcDNA3-EFNB2-RBD-Fc (WT)

   Plasmid

   #200978

   Purpose

   Mammalian expression plasmid for soluble EFNB2 (wild type) fused to IgG1 Fc

   Depositor

   Erik Procko

   Article

   Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

   Insert

   EFNB2 (EFNB2 Human)
   

   Use

   Tags

   IgG1 Fc

   Expression

   Mammalian

   Mutation

   Soluble Receptor-Binding Domain

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3 Flag MKK7B2Jnk1a1

   Plasmid

   #19726

   Depositor

   Roger Davis

   Article

   Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

   Insert

   MKK7B2Jnk1a1 (Map2k7, MAPK8 Mouse, Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA HA HP1 beta

   Plasmid

   #24079

   Depositor

   Naoko Tanese

   Article

   Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

   Insert

   HP1 beta (CBX1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-EGFP-Rac1-wt

   Plasmid

   #12980

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. opto-RhoGEF-mCherry_pcDNA3.1

   Plasmid

   #164473

   Purpose

   Expresses opto-RhoGEF (BcLOV4-GGGSx2-ARHGEF11dh-GGGSx2-mCherry) in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al Adv Biol. 2021 Sep;5(9):e2100810. doi: 1

   Insert

   opto-RhoGEF
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1+/-FLI1-C‐(K)‐DYK

   Plasmid

   #129497

   Purpose

   Mammalian expression of human FLI1

   Depositor

   Marjorie Brand

   Article

   Palii et al Cell Stem Cell. 2019 May 2;24(5):812-820

   Insert

   FLI1 (FLI1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-IDH2-R172K

   Plasmid

   #87927

   Purpose

   Expresses IDH2 (R172K mutant) in mammalian cells

   Depositor

   Reproducibility Project Cancer Biology

   Article

   Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

   Insert

   isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   R172K

   Promoter

   CMV

   Available since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-hRIPK1-Flag

   Plasmid

   #112487

   Purpose

   Mammalian expression of Flag-hRIPK1. Please note Flag tag is N-terminal.

   Depositor

   Junying Yuan

   Insert

   RIPK1 (RIPK1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-10His-StayGold

   Plasmid

   #211362

   Purpose

   Mammalian expression of the bright and photostable StayGold fluorescent protein. Contains a N-terminal 10xHis tag.

   Depositor

   Mohan Balasubramanian

   Article

   Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

   Insert

   StayGold
   

   Use

   Tags

   10xHis-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 CLN3-GFP

    Plasmid

    #78110

    Purpose

    Mammalian expression of human CLN3 with C terminal GFP fusion

    Depositor

    Thomas Braulke

    Insert

    CLN3 (CLN3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3 Hygro HA Akt2

    Plasmid

    #16000

    Depositor

    Morris Birnbaum

    Insert

    Akt2 (AKT2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3. Flag-CSL WT

    Plasmid

    #167004

    Purpose

    Mammalian expression of CSL WT

    Depositor

    Gian-Paolo Dotto

    Article

    Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

    Insert

    CSL (RBPJ Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-Hyg-RFPKDEL

    Plasmid

    #138660

    Purpose

    Reporter plasmid expressing the ER-located RFP

    Depositor

    Bertrand Collet

    Article

    Dehler et al J Immunol. 2019 Jul 15;203(2):465-475. d

    Insert

    RFP-KDEL
    

    Use

    Tags

    KDEL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 VP4-VP2

    Plasmid

    #216787

    Purpose

    Mammalian expression of the VP4-VP2 portion of the CVB3 Kandolf strain polyprotein

    Depositor

    Kevin Janes

    Insert

    VP4-VP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-Casp7-Flag

    Plasmid

    #11815

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 7 (CASP7 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BcLOV4-mammalian-recoded_mCherry_pcDNA3.1

    Plasmid

    #114595

    Purpose

    Encodes mCherry-tagged BcLOV4 for expression in mammalian cells

    Depositor

    Brian Chow

    Article

    Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

    Insert

    BcLOV4
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA-mCherry-4A/4B-CAAX

    Plasmid

    #87699

    Purpose

    Express mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    mCherry-4A/4B-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Flag-RNF125

    Plasmid

    #122045

    Purpose

    express RNF125

    Depositor

    Ze'ev Ronai

    Insert

    RNF125 (RNF125 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3-Flag-SPRTN-wt

    Plasmid

    #110214

    Purpose

    Expression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.

    Depositor

    Ivana Marinovic-Terzic, Kristijan Ramadan

    Article

    Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P296L (see depositor comments below)

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-GGamma13-GFP2

    Plasmid

    #140992

    Purpose

    Encodes a G gamma subunit (GNG13) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma13-GFP2 (GNG13 Human)
    

    Use

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1(+) K-Ras(G12C)

    Plasmid

    #224284

    Purpose

    Cell transfection and expression of K-Ras (G12C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    K-Ras (KRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G12C

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDNA3.1-HsPcdh21-mNeonGreen

    Plasmid

    #210819

    Purpose

    Mammalian overexpression vector for C-terminally mNeonGreen tagged human Pchd21 (WT)

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    Protocadherin 21 (CDHR1 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3 p53 S15A

    Plasmid

    #69004

    Purpose

    mammalian expression of human p53 S15A mutant.

    Depositor

    David Meek

    Article

    Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S15A

    Promoter

    CMV

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-MBII85-5'SS mut

    Plasmid

    #67631

    Purpose

    MBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons. To improve snoRNA expression efficiency 5' splice site was mutated to consensus sequence.

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

    Insert

    MBII85 snoRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutation in 5'ss

    Promoter

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-Cyto-CaNAR2

    Plasmid

    #64729

    Purpose

    Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNAR2-NES
    

    Use

    Tags

    Cerulean3, Nuclear export signal (NES), and YPet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Dead-Laconic/pcDNA3.1(-)

    Plasmid

    #118627

    Purpose

    To express in mammalian cells a lactate FRET sensor negative control.

    Depositor

    Luis Felipe Barros

    Article

    Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:

    Insert

    Dead-Laconic
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Change Histidine 151 to Aspartic Acid of Escheric…

    Promoter

    CMV Promoter

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3 Ash2L full

    Plasmid

    #15548

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    Ash2L (ASH2L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R622H (Please see depositor comments below)

    Promoter

    Available since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-Cry2FL-VP64

    Plasmid

    #60554

    Purpose

    Expresses Cry2FL-VP64 in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    Cry2FL-VP64
    

    Use

    Tags

    FLAG, HA tag, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA-SUN2 mApple

    Plasmid

    #187679

    Purpose

    In vivo visualization of SUN2

    Depositor

    Mary Beckerle

    Insert

    SUN2 (Sun2 Mouse)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3 MYC-Sumo3Q89P

    Plasmid

    #48963

    Purpose

    Expresses MYC-Sumo3Q90P mutant in mammalian cells

    Depositor

    Guy Salvesen

    Article

    Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

    Insert

    SUMO3 (SUMO3 Human)
    

    Use

    Tags

    MYC (EQKLISEED)

    Expression

    Mammalian

    Mutation

    Mutated Q89P

    Promoter

    T7

    Available since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

31. pcDNA3 CDK9 HA (P#635)

    Plasmid

    #14640

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

    Insert

    cdk9 (CDK9 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3-PBD-EYFP

    Plasmid

    #13723

    Depositor

    Klaus Hahn

    Article

    Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

    Insert

    PBD-EYFP (PAK1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    p21 binding domain of human Pak1(amino acids 65-1…

    Promoter

    Available since

    March 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. TRAPPC10_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176989

    Purpose

    Mammalian expression vector encoding TRAPPC10 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    TRAPPC10 (TRAPPC10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA6 asyn A30P

    Plasmid

    #105729

    Purpose

    Mammalian expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

    Insert

    alpha-synuclein A30P (SNCA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A30P

    Promoter

    CMV

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1-CHRNA7-mCherry

    Plasmid

    #62632

    Purpose

    mammalian expression of human Alpha7 (CHRNA7) with mCherry fused into M3-M4 loop

    Depositor

    Henry Lester

    Article

    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

    Insert

    hAlpha7 (CHRNA7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mCherry inserted into M3-M4 loop

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Academic Institutions and Nonprofits only

36. pcDNA-Flag-Kdm4b-pA

    Plasmid

    #104486

    Purpose

    Express mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.

    Depositor

    Yi Zhang

    Article

    Inoue et al Genes Dev. 2017 Oct 1;31(19):1927-1932.

    Insert

    Kdm4b (Kdm4b Mouse)
    

    Use

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3 IKKe flag

    Plasmid

    #26201

    Depositor

    Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    I-kappa-B kinase epsilon (IKBKE Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-ExRai-AktAR

    Plasmid

    #118462

    Purpose

    cpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AktAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1-MS2-HA-HsRck_AC

    Plasmid

    #148417

    Purpose

    Mammalian Expression of HsRck

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    HsRck (DDX6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1-TEK-V5/HIS

    Plasmid

    #205611

    Purpose

    Expression of human TEK receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    TEK receptor tyrosine kinase (TEK Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge