We narrowed to 3,395 results for: zebrafish
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Plasmid#218207PurposeZebrafish -2.1prox1a_1 (mouse conserved) enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a_1
UseZebrafish expressionAvailable SinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSlick-Neo-RECK
Plasmid#121986PurposeTetracycline-inducible expression of untagged full-length RECKDepositorAvailable SinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
AsCas12a-2C-NLS in pCSDest
Plasmid#126637PurposeExpresses AsCas12a-2C-NLS in mammalian cellsDepositorInsertAsCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianPromoterCMV IE94 promoterAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mVenus1-155-GGGS
Plasmid#162610PurposeAllows for transcription of control mVenus fluorophore half for injection into zebrafish embryos for BiFC assaysDepositorInsertmVenus
ExpressionMammalianMutationContains aa1-155 of mVenusAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-Y276H PHYB-CAAX
Plasmid#154911Purpose1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine.DepositorInsertPhytochrome B (PHYB Mustard Weed)
UseXenopus/avian/zebrafishTagsCAAX membrane moiety and mCherryExpressionMammalianMutation1-621 amino acids of Arabidopsis PHYB, Tyrosine r…PromoterCMVAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
rag2:MYRFL_CE_pISceI
Plasmid#223045PurposeExpress MYRFL gene in mesenchymal lineage of Zebrafish.DepositorInsertMYRFL
UseExpress the insert(gene) in mesenchymal lineage o…PromoterRag2Available SinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
Danio alpha A-crystallin 1kb promoter/AcGFP
Plasmid#98096PurposeaA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryosDepositorInsertAlpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)
UseGfp expressingTagsGFPPromoterDanio alpha A-crystallin 1 kb fragment (-1028/-1)Available SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG657 H2B-pFAST
Plasmid#172862PurposeExpresses pFAST fused to zebrafish histone H2B in mammalian cellsDepositorInsertH2B-pFAST
TagsMyc tag (C terminal on insert)ExpressionMammalianPromoterCMVAvailable SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-Avi-Cerulean-RanGAP
Plasmid#79878PurposeBeta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
TagsAviExpressionBacterialPromoterBeta-actin2Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
ACVR1-TAAS-R206H
Plasmid#206184Purposein vitro transcription of RNA encoding human ACVR1 with mutations S190A, S192A, and R206HDepositorInsertactivin A receptor type 1 (ACVR1 Human)
UseIn vitro transcriptionTagsFLAG tagMutationS190A, S192A, R206HAvailable SinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mVenus1-155-I152L-GGGS
Plasmid#162613PurposeAllows for transcription of improved control mVenus I152L fluorophore half for injection into zebrafish embryos for BiFC assaysDepositorInsertmVenus
ExpressionMammalianMutation1-155-I152LAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
FnoCas12a-2C-NLS in pCSDest
Plasmid#126639PurposeExpresses FnoCas12a-2C-NLS in mammalian cellsDepositorInsertFnoCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianPromoterCMV IE94 promoterAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-CDC42
Plasmid#109531PurposeMuscle specific zebrafish expression of mKate2 fused to CDC42DepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-CDC42-DN
Plasmid#109532PurposeMuscle specific zebrafish expression of mKate2 fused to dominant negative CDC42DepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T-Easy-NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT
Plasmid#119067PurposeDonor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombinationDepositorInsertNLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT
ExpressionBacterialAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-cytoBirA-2A-mCherry-SV40pA-FKF
Plasmid#79887PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
ExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T-Easy-HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT
Plasmid#119065PurposeDonor construct to amplify HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombinationDepositorInsertHA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT
TagsHAExpressionBacterialAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-SERCA1
Plasmid#109498PurposeMuscle specific zebrafish expression of mKate2 fused to sarcoplasmic/endoplasmic calcium ATPase 1DepositorInsertSERCA1 (atp2a1 Zebrafish)
UseZebrafish plasmidsTagsmKate2MutationPlease see Depositor CommentsAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-DR-GC-swap-cr in pBCSK+
Plasmid#126643PurposeCloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cellsDepositorInsertLbCas12a Full Length Direct Repeat GC Swap crRNA
UseCRISPRExpressionMammalianPromoterU6 PromoterAvailable SinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT-flk1-cytoBirA-2A-mCherry_Ras
Plasmid#80056PurposeFlk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
Tags3x HAExpressionBacterialPromoterflk1Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCK072 p5E 147bp exorh:EGFP
Plasmid#195950PurposeGateway p5E vector with reporterDepositorInsertexorh minimal regulatory elements and EGFP
UseOtherAvailable SinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-NLS-BirA-2a-mCherry
Plasmid#79886PurposeUbiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
Tags3x HAExpressionBacterialPromoterUbiquitinAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-Avi-Cerulean-RanGAP
Plasmid#79881PurposeUbiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
TagsAviExpressionBacterialPromoterUbiquitin promoterAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-foxc1a
Plasmid#132971Purposefull-length of foxc1a without stop codon in pmE Gateway vectorDepositorInsertfoxc1a (foxc1a Zebrafish)
UseGateway vectorAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS-foxc1a-2a-nlstdTomato
Plasmid#132970Purposefull-length of foxc1a in frame with viral 2a peptide and nuclear localized tdTomato in pCS expression vectorDepositorAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-EEA1
Plasmid#109508PurposeMuscle specific zebrafish expression of mKate2 fused to early endosome associated 1DepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
ND4-FusXTBE-Left TALE
Plasmid#196496PurposeExpresses left-side TALE-DddAtox Cterm half ND4-FusXTBE in mammalian cellsDepositorInsertCOX8A MTS-ND4 left TALE-G1397 DddA-C-UGI-ATP5B 3'UTR
ExpressionMammalianAvailable SinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
ND4-FusXTBE-Right TALE
Plasmid#196497PurposeExpresses Right-side TALE-DddAtox Nterm half ND4-FusXTBE in mammalian cellsDepositorInsertCOX8A MTS-ND4 right TALE-G1397 DddA-C-UGI-ATP5B 3'UTR
ExpressionMammalianAvailable SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF
Plasmid#119864PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertBirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
TagsHA-tagged biotin ligase (BirA), Thosea asigna vir…ExpressionBacterialAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTol2-PcyA-IRES-HO1
Plasmid#131787PurposeTol2 bicistronic vector expressing PcyA and HO1 for Phycocyanobilin (PCB) synthesis, driven by heatshock promoter hsp70.DepositorInsertPcyA and HO1
UseSynthetic Biology; Zebrafish tol2 transposonTagsMTS (Mitochondrial Targeting Signal from subunit …ExpressionMammalianPromoterhsp70Available SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
bactin:mKate2-ras
Plasmid#105963Purposetransgenesis, ubiquitous expressionDepositorAvailable SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Danio alpha Bb-crystallin 1kb promoter/AcGFP
Plasmid#98098PurposeaBb-crystallin promoter fragment in GFP plasmid to assess activity in larval embryosDepositorInsertAlpha Bb-crystallin promoter 1 kb fragment, Danio rerio (cryabb Zebrafish)
UseGfp expressingTagsGFPPromoterDanio aBb-crystallin 1 kb fragment (-1074/-1)Available SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF
Plasmid#79889PurposeDonor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
ExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT
Plasmid#89890PurposeBAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.DepositorInsertHA-BirA-2A-citrine
UseUnspecifiedTagsCitrineAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMini-CAAGs::RFP-DR48
Plasmid#132640PurposeFluorescent reporter to quantify elicitation of the strand annealing mediated repair pathwayDepositorInsertmRFP
ExpressionMammalianMutationContains a 92bp insertion interrupting the open …PromoterChicken B-actinAvailable SinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
FnoCas12a-DR-GC-swap-cr in pBCSK+
Plasmid#126644PurposeCloning vector FnoCas12a-DR-GC-swap-crRNA for expression in mammalian cellsDepositorInsertFnoCas12a Full Length Direct Repeat GC Swap crRNA
UseCRISPRExpressionMammalianPromoterU6 PromoterAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-ARF1-DN
Plasmid#109536PurposeMuscle specific zebrafish expression of mKate2 fused to dominant negative ARF1DepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Danio alpha Ba-crystallin 3kb promoter/AcGFP
Plasmid#98097PurposeaBA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryosDepositorInsertAlpha Ba-crystallin promoter 3 kb fragment, Danio rerio (cryaba Zebrafish)
UseGfp expressingTagsGFPPromoterDanio alpha Ba-crystallin 3 kb fragment (-3000/-1)Available SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-CoCHR-mcherry
Plasmid#254785PurposemCherry fused optogenetic opsin CoChR overexpression in zebrafish neutrophils.DepositorInsertCoCHR-mCherry
UseZebrafish expressionPromoterLyzcAvailable SinceJune 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-GtACR2-tdTomato
Plasmid#254783PurposetdTomato fused optogenetic opsin GtACR2 overexpression in zebrafish neutrophils.DepositorInsertGtACR2-tdTomato
UseZebrafish expressionPromoterLyzcAvailable SinceMay 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-BLINK2-2a-mcherry caax
Plasmid#254784PurposeOptogentic tool BLINK2 overexpression in zebrafish neutrophils with membrane label.DepositorInsertBLINK2-2A-mCherry-CAAX
UseZebrafish expressionPromoterLyzcAvailable SinceMay 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-GFP-2a-PTX
Plasmid#254788PurposePertussis toxin (PTX) overexpression in GFP tagged zebrafish neutrophilsDepositorInsertPTX
UseZebrafish expressionPromoterLyzcAvailable SinceMay 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-PHAKT-mCerulean3
Plasmid#254786PurposeMembrane PIP3 level reporter of zebrafish neutrophilsDepositorInsertPHAKT-mCerulean3
UseZebrafish expressionPromoterLyzcAvailable SinceMay 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CR-QUAS:mNeonGreen-NES-P2A-mKate2-NLS
Plasmid#241912PurposeZebrafish transgenesis plasmid encoding cytoplasmic mNeonGreen-NES and mKate2-NLS separated by the P2A peptide, driven by the QUAS enhancer, and including a cmlc2:mKate2 cardiac transgenesis markerDepositorInsertQUAS enhancer driving mNeonGreen-NES and mKate2-NLS
UseZebrafish tol2 transgenesisPromoterQUASAvailable SinceOct. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2-QUAS:cPla2-mKate2
Plasmid#241913PurposeZebrafish transgenesis plasmid encoding D. rerio cPla2-mKate2, driven by the QUAS enhancer, and including a cmlc2:EGFP cardiac transgenesis markerDepositorInsertQUAS enhancer driving cPla2-mKate2
UseZebrafish tol2 transgenesisTagscPla2 fused to mKate2PromoterQUASAvailable SinceOct. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CR-QUASM1:GCaMP7s-NLS-P2A-mKate2-NLS
Plasmid#241915PurposeZebrafish transgenesis plasmid encoding GCaMP7s-NLS and mKate2-NES separated by the P2A peptide, driven by the QUASM1 enhancer, and including a cmlc2:mKate2 cardiac transgenesis markerDepositorInsertQUASM1 enhancer driving GCaMP7s-NLS and mKate2-NLS
UseZebrafish tol2 transgenesisPromoterQUASM1Available SinceOct. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
Nanog-mNG
Plasmid#238296PurposeFor RNA injection of Nanog-mNG into ZebrafishDepositorInsertNanog-mNG
UseZebrafish expressionAvailable SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAX1_Z11 gpN:SpyTag
Plasmid#225196PurposeUsed for adding C-terminal SpyTag to the Plesiomonas ZOR0011 P2 phage major capsid proteinDepositorInsertgpN homology arms and SpyTag
ExpressionBacterialAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAX1_HS_lexA(Ind-)
Plasmid#225199PurposeUsed to create a non-inducible lexA by introducing a G85D mutation in E. coli HSDepositorInsertlexA homology arms
ExpressionBacterialMutationlexA G85DAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
rag2:METTL121B_CE_pISceI
Plasmid#223030PurposeExpress METTL121B gene in mesenchymal lineage of Zebrafish.DepositorInsertMETTL121B
UseExpress the insert(gene) in mesenchymal lineage o…PromoterRag2Available SinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only