We narrowed to 18,496 results for: Met

1. pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2

   Plasmid

   #169531

   Purpose

   Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven nucleus-localized monomeric Tag blue fluorescent protein 2
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pG23A

   Plasmid

   #102884

   Purpose

   Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Insert

   Cdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
   

   Tags

   AID(71-114) and mCherry

   Expression

   Yeast

   Promoter

   Native promoter

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pzyxin-emiRFP703

   Plasmid

   #136568

   Purpose

   Zyxin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   zyxin-emiRFP703
   

   Tags

   emiRFP703

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. mADAM17deltaMP in pQCXIP cFLAG

   Plasmid

   #19144

   Depositor

   Zena Werb

   Article

   Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

   Insert

   ADAM17 (Adam17 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   delta 189-378

   Available Since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pXWJ109AsAmp30E

   Plasmid

   #123144

   Purpose

   Arsenic sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J109, ParsR, PhrpLE

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT2SCb

   Plasmid

   #59385

   Purpose

   Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.

   Depositor

   Shelley Copley

   Article

   Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

   Insert

   TT-ISceI-cat2
   

   Use

   Template

   Mutation

   The 3’-end of the chloramphenicol resistance gene…

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUBQ10::ZF108_3xFLAG_YPet

   Plasmid

   #106441

   Purpose

   pUBQ10 driven Zinc Finger YPet fusion that targets the FWA promoter in arabidopsis

   Depositor

   Steven Jacobsen

   Article

   Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

   Insert

   ZF108_3xFLAG_YPet
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pY012 (pcDNA3.1-hCMtCpf1)

   Plasmid

   #69984

   Purpose

   Expresses humanized CMtCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hCMtCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. MAC-SARS-CoV-2 Orf6

   Plasmid

   #158374

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   SARS-CoV-2 Orf6 (ORF6 )
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Available Since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pBABE-Src-Rescue

    Plasmid

    #26983

    Depositor

    Joan Massague

    Article

    Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

    Insert

    c-Src (CSK Chicken)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pBS SK mCherryROSAbsr

    Plasmid

    #54321

    Purpose

    Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.

    Depositor

    Silvestro Conticello

    Article

    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

    Insert

    mCherry and BlasticidinS-resistance
    

    Use

    CRISPR, Mouse Targeting, and TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. psiCHECK2-miR-34 MT

    Plasmid

    #78259

    Purpose

    psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

    Depositor

    Joanne Weidhaas

    Article

    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

    Insert

    mutated miR-34 target site
    

    Use

    Luciferase; Microrna activity

    Expression

    Mammalian

    Mutation

    nucleotides 2-5 and 10-11 changed from CCGT to GG…

    Available Since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTS2396-Tier1-PhCMV-4xCOX8_Ypet

    Plasmid

    #169534

    Purpose

    Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven miochondria-localized yellow fluorescent protein Ypet
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. MAC-SARS-CoV-2 E

    Plasmid

    #158368

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 E (E )
    

    Tags

    MAC-tag

    Expression

    Mammalian

    Available Since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. GFP-LacI-p300

    Plasmid

    #179270

    Purpose

    Expresses GFP-LacI-p300 fusion plasmid

    Depositor

    Lienhard Schmitz

    Article

    Pfisterer et al Methods Mol Biol. 2023;2589:361-376. doi

    Insert

    E1A binding protein p300 (EP300 Human)
    

    Tags

    GFP-LacI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. KHBD00222

    Plasmid

    #34370

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG1832 (CG1832 Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pLKO.1-puro-PARG shRNA

    Plasmid

    #23260

    Depositor

    Robert Sobol

    Article

    Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

    Insert

    poly(ADP-ribose) glycohydrolase shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

18. AYBEv0.1

    Plasmid

    #193966

    Purpose

    A wildtype adenine transversion base editor (AYBEv0.1, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    ecTadA8e-nSpCas9(D10A)-MPG(wt)
    

    Expression

    Mammalian

    Promoter

    hU6, EF1a, CBh

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3.0_mitoLAMA-F98 IRES EGFP

    Plasmid

    #130706

    Purpose

    Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    NTOM20-SNAP-Tag-LAMA-F98 IRES EGFP
    

    Tags

    NTOM20 and SNAP-Tag

    Expression

    Mammalian

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICE-EGFP-FLAG-Ku70siR-WT

    Plasmid

    #46961

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    human Ku70 (XRCC6 Human)
    

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Promoter

    CMV Tet

    Available Since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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