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pCIneoRL-BCL2 3'UTR

Plasmid

#84597

Purpose

Renilla luciferase reporter containing BCL2 3' UTR

Depositor

Yanan Yang

Article

Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

Insert

BCL2 3'UTR (UTS2R Human)

Use

Luciferase

Tags

Renilla Luciferase

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA-mCherry-4A/4B-CAAX

Plasmid

#87699

Purpose

Express mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

mCherry-4A/4B-CAAX

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-TYK2

Plasmid

#139354

Purpose

Expression of TYK2 protein fused with GFP

Depositor

Sandra Pellegrini

Article

Ragimbeau et al J Biol Chem. 2001 Aug 17;276(33):30812-8

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLGR002 LGR Cas9 guide vector

Plasmid

#188320

Purpose

Universal backbone for CRISPR/Cas9 sgRNA expression

Depositor

Laralynne Przybyla

Article

Heo et al Genome Biol. 2024 Jan 19;25(1):25. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

PuroR-T2A-mTagBFP2

Expression

Mammalian

Promoter

pU6 5'CMV and EF1-alpha

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pQTEV-ASF1A

Plasmid

#31591

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pME map2k2a_s219D

Plasmid

#201058

Purpose

Gateway middle entry vector expressing constitutively active zebrafish map2k2a

Depositor

Sarah Childs

Article

Greysson-Wong et al Development. 2023 Sep 15;150(18):dev2018

Insert

MAP2k2a (map2k2a Zebrafish)

Use

Zebrafish expression

Mutation

Mutation S219D, constitutively active

Promoter

none

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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p8325 pLIX YAP1 WT

Plasmid

#184527

Purpose

Expression of YAP1 WT

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

YAP1 WT (YAP1 Human)

Use

Lentiviral

Promoter

TRE promoter, Tet ON

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMW364

Plasmid

#238205

Purpose

Expresses untagged TasR from a PBAD promoter, followed by 3xRNA1 TIGR array

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Expression

Bacterial

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLV.PARP1#1

Plasmid

#14544

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

Insert

PARP1 shRNA (PARP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 27, 2007

Availability

Academic Institutions and Nonprofits only
pBabe-puro-miR-9 sponge

Plasmid

#25040

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

miR-9 sponge

Use

Retroviral

Tags

d2EGFP

Expression

Mammalian

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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pUC57-attB2-SA(2)-CRY2-mCherry

Plasmid

#160441

Purpose

To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 2.

Depositor

Nicholas Tolwinski

Article

Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Insect

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD1

Plasmid

#195839

Purpose

Expresses human FZD1 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD1 (FZD1 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLV.PARP1#2

Plasmid

#14545

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

Insert

PARP1 shRNA (PARP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 27, 2007

Availability

Academic Institutions and Nonprofits only
hDAGLa-V5

Plasmid

#87676

Purpose

Expresses human DAGL? protein with intracellular V5 tag located on the C-terminal

Depositor

Pat Doherty

Article

Singh et al Biosci Rep. 2016 May 6;36(3). pii: e0033

Insert

Human Diacylglycerol Lipase Alpha (DAGLA Human)

Tags

V5 tag

Expression

Mammalian

Promoter

T7

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLM-fSV2A

Plasmid

#27512

Depositor

Michel Sadelain

Article

Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

Insert

OCT4_T2A_KLF4_P2A_cMYC_E2A_Sox2

Use

Lentiviral

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pFX-β-tubulin-EGFP

Plasmid

#175827

Purpose

Express EGFP with microtubule-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with microtuble-targeting sequence

Tags

human tubulin beta 4B class IVb;TUBB4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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FUGW U6 gL1HSg2dCas9-KRAB-T2a-GFP

Plasmid

#234881

Purpose

L1HS-silencing plasmid (CRISPRi gRNA2)

Depositor

Johan Jakobsson

Article

Adami et al bioRxiv 2025.01.17.633315

Insert

LacZ gRNA

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pTS2355_Tier3(PB)-iRFP_PuroR

Plasmid

#169654

Purpose

Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PRPBSA-driven iRFP670 and PuroR expression

Expression

Mammalian

Promoter

PRPBSA

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TetON-Bmpr1A-ca

Plasmid

#217536

Purpose

AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged constitutively-active BMPR1A receptor

Depositor

Peter Scheiffele

Article

Okur et al bioRxiv 2023

Insert

constitutively active BMP receptor 1a (BMPR1A Human)

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

Tet operon (TetO)

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-TO-Vpr-mRuby3-IN

Plasmid

#167584

Purpose

For producing fluorescently labeled HIV-1; expresses HIV-1 integrase fused to Vpr for packaging into HIV-1 and the fluorophore mRuby3

Depositor

Zandrea Ambrose

Article

Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

Insert

Vpr-mRuby3-IN

Tags

Vpr-mRuby3

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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