165,725 results

1. pAS3-lim-4P_gtACR2_SL2_eGFP(65C)_unc-54 3'UTR

   Plasmid

   #195853

   Purpose

   Expresses inhibitory opsin gtACR2 and GFP in turning associated neurons

   Depositor

   Andrew Leifer

   Article

   Kumar et al PLoS Biol. 2023 Sep 21;21(9):e3002280. d

   Insert

   Guillardia theta anion-conducting channelrhodopsin-2 (GtACR2)
   

   Expression

   Worm

   Promoter

   lim-4

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_Linear Tau 0N4R 3X Flag tag

   Plasmid

   #194171

   Purpose

   Insert contains linear tau 0N4R with a 3X Flag tag. Expresses linear protein tau 0N4R with a 3X Flag tag for Immunoprecipitation.

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   Microtubule-Associated Protein Tau 0N4R
   

   Tags

   3X Flag

   Expression

   Mammalian

   Available Since

   Jan. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Golgi-TurboID

   Plasmid

   #200959

   Purpose

   Transiently expresses golgi localized TurboID with HA tags

   Depositor

   Keriann Backus

   Article

   Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

   Insert

   Golgi-TurboID
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHPHS800

   Plasmid

   #228240

   Purpose

   Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination

   Depositor

   Arvind Rasi Subramaniam

   Article

   Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

   Insert

   Cas9
   

   Tags

   Hygromycin phosphotransferase

   Expression

   Mammalian

   Promoter

   none

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-EF1a-H2B-miRFP670

   Plasmid

   #212668

   Purpose

   Express tagged H2B

   Depositor

   Tobias Meyer, Yumi Konagaya

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   H2B (H2BC11 Human)
   

   Use

   Lentiviral

   Tags

   miRFP670

   Promoter

   EF-1a

   Available Since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PMmEos3.2

   Plasmid

   #203755

   Purpose

   Encodes the membrane anchor of Lyn including the first 10 residues of the N terminus, with 1 myristoylation and 1 palmitoylation, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   Lyn n-term (LYN Human)
   

   Tags

   mEos3.2

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPtGE34

   Plasmid

   #107932

   Purpose

   Expresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

   Depositor

   David Edgell

   Article

   Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

   Inserts

   OriT
   40SRPS8 Promoter
   ShBle
   40SRPS8 Terminator
   Cen6-ArsH4-His3
   

   Use

   CRISPR and Synthetic Biology; Episomal vector for…

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W

   Plasmid

   #67982

   Purpose

   Cas9 activity reporter with mCherry and GFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Mutation

   Deleted BbsI site within WPRE

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-Rubicon

   Plasmid

   #28022

   Depositor

   Qing Zhong

   Article

   Sun et al J Biol Chem. 2011 Jan 7. 286(1):185-91.

   Insert

   Rubicon (RUBCN Human)
   

   Tags

   3xFlag and EGFP

   Expression

   Mammalian

   Available Since

   March 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR-attL5-CRY2-mCh-attL2

    Plasmid

    #160438

    Purpose

    Entry vector for cloning Cryptochrome2-mCherry using 2-fragment gateway recombination

    Depositor

    Nicholas Tolwinski

    Article

    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

    Insert

    Cryptochrome 2 (CRY2 Mustard Weed)
    

    Tags

    mCherry

    Expression

    Bacterial

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmiRGLO Dual-Luciferase-PDAP1-3'UTR

    Plasmid

    #182251

    Purpose

    Human PDAP1 3` UTR region containing 4x binding sites for miR-150 cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

    Insert

    3' UTR region of PDAP1 gene
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV x EF1a_EKAREN5-ires-puro

    Plasmid

    #167821

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. HyperNova/pRSETB

    Plasmid

    #221456

    Purpose

    Fast-maturating photosensitizing fluorescent protein for chromophore-assisted light inactivation

    Depositor

    Kiwamu Takemoto

    Article

    Shidara et al Commun Biol. 2024 Aug 6;7(1):945. doi: 1

    Insert

    HyperNova
    

    Expression

    Bacterial

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19/PLIN2 GFP-P2A-BFP

    Plasmid

    #202195

    Purpose

    knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

    Depositor

    James Olzmann

    Article

    Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

    Insert

    PLIN2 (PLIN2 Human)
    

    Use

    Cloning vector

    Tags

    GFP-P2A-BFP knock-in

    Promoter

    lac

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3/Flag-WTAP

    Plasmid

    #53741

    Purpose

    For expression of WTAP (Wilms tumor 1 associated protein) in human cells

    Depositor

    Chuan He

    Article

    Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

    Insert

    Wilms tumor 1 associated protein (WTAP Human)
    

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCZGY2254

    Plasmid

    #101247

    Purpose

    GFP subunits 1-10, (only shows active fluorescence when binds to GFP11)

    Depositor

    Yishi Jin

    Article

    Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

    Insert

    gfp1-10
    

    Use

    Gateway

    Tags

    GFP1-10

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiCRISPR-V2_hMSH2

    Plasmid

    #186155

    Purpose

    sgRNA targeting human MSH2 gene

    Depositor

    David McFadden

    Article

    Povedano et al Cell Chem Biol. 2019 Sep 19;26(9):1315-1

    Insert

    MSH2 (MSH2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEFIRES-P-EGFP-ADRP

    Plasmid

    #87157

    Purpose

    Fluorescent protein localized to lipid droplets (LDs)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    ADRP (PLIN2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    mCherry-R TTGGTCACCTTCAGCTTGG

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-Akt-FoxO3a-KTR-EGFP

    Plasmid

    #125131

    Purpose

    Fluorescent reporter for Akt activity

    Depositor

    Kazuhiro Aoki

    Article

    Maryu et al Cell Struct Funct. 2016 Jul 22;41(2):81-

    Insert

    FOXO2 (FOXO3 Human)
    

    Tags

    Enhanced green fluorescent protein (EGFP)

    Expression

    Mammalian

    Mutation

    FoxO3A 1-402 amino acid

    Promoter

    CAG promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-TXNIP

    Plasmid

    #18758

    Depositor

    Clark Distelhorst

    Article

    Wang et al Oncogene. 2006 Mar 23. 25(13):1903-13.

    Insert

    thioredoxin-interacting protein (Txnip Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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