9,000 results

Showing: 1041 - 1060 of 9000 results

Results per page:

Sort results by:

pHR-SFFV-dCas9-BFP-KRAB

Plasmid

#46911

Purpose

Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9-BFP-KRAB fusion

Use

CRISPR and Lentiviral

Tags

2xNLS, BFP, HA, and KRAB domain

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiGuide Blast

Plasmid

#199622

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.

Depositor

Danwei Huangfu

Article

Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pK168.AAV-EF1α-DIO-tTA-P2A-RFP-WPRE (Supernova)

Plasmid

#85039

Purpose

For AAV-TRE-Cre based Supernova-RFP, pK168 should be used with pK170.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

RFP-P2A-tTA

Use

AAV

Expression

Mammalian

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PCR4-Shh::lacZ-H11

Plasmid

#139098

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

Depositor

Len Pennacchio

Article

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

Shh

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTriExRhoA2G

Plasmid

#40176

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

RhoA Biosensor

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-7 asyn WT

Plasmid

#36046

Depositor

Hilal Lashuel

Article

Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

Insert

alpha-synuclein (SNCA Human)

Expression

Bacterial

Promoter

T7

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b_Bst-LF

Plasmid

#153313

Purpose

Bacterial expression of the Bst Large Fragment

Depositor

Drew Endy, Philippa Marrack

Insert

Bst Large Fragment

Expression

Bacterial

Available Since

June 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHR_Gal4UAS_tBFP_PGK_mCherry

Plasmid

#79130

Purpose

Lentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherry

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

tBFP

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSL1142 (pSPIN, pBBR1 backbone)

Plasmid

#160730

Purpose

Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a-alpha-synuclein

Plasmid

#51486

Purpose

Inducible expression of human alpha-synuclein in E.coli

Depositor

Michael J Fox Foundation MJFF

Insert

alpha-synuclein (SNCA Human)

Expression

Bacterial

Promoter

T7

Available Since

March 31, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMOS023: Peredox NADH/NAD+ sensor (cytosolic)

Plasmid

#163060

Purpose

Peredox NADH/NAD+ sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Peredox

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
HR180-LGR5-iCT

Plasmid

#129094

Purpose

KI iCaspase9-tdTomato with a puromycin/mRuby selection cassette into human LGR5 locus

Depositor

Toshiro Sato

Article

Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

Insert

iCaspase9-T2A-tdTomato and LGR5 homology arms (LGR5 Human)

Use

Homologous recombination donor plasmid

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

Plasmid

#67980

Purpose

Cas9 activity reporter with BFP and GFP.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKBFP2AGFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
p6XHis-T7(P266L)

Plasmid

#174866

Purpose

Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.

Depositor

Anna Pyle

Article

Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.

Insert

T7 RNA polymerase

Tags

6XHis-tag

Expression

Bacterial

Mutation

P266L

Promoter

lac promoter

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PH-Akt-GFP

Plasmid

#51465

Purpose

Is a biosensor for PIP3/PI(3,4)P2

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

AKT (aa 1–164)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRK892

Plasmid

#71803

Purpose

Expression of mutated L. gasseri gusA from strong Ppgm promoter

Depositor

Rodolphe Barrangou, Todd Klaenhammer

Article

Duong et al Microb Biotechnol. 2011 May;4(3):357-67.

Insert

gusA3

Expression

Bacterial

Mutation

D573A

Promoter

Ppgm

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-PP7-2xmCherry-CAAX

Plasmid

#74925

Purpose

Expresses PP7-2xmCherry-CAAX

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

PP7-2xmCherry-CAAX

Use

Lentiviral

Expression

Mammalian

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_Gal4UAS_tBFP_PGK_mCitrine

Plasmid

#188386

Purpose

Lentiviral vector - tBFP reporter for Gal4-based SNIPRs with a constitutive mCitrine;

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

Gal4UAS_tBFP_PGK_mCitrine

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET GFP LIC cloning vector (2GFP-T)

Plasmid

#29716

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, SiriusGFP, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA GNSTM-3-RVG-10-Lamp2b-HA

Plasmid

#71294

Purpose

Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

Depositor

Joshua Leonard

Article

Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

Insert

Lamp2b (LAMP2 Human)

Tags

GNSTM glycosylation motif, HA, Lamp2 signal pepti…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge