We narrowed to 9,223 results for: Coli

1. pHE-5

   Plasmid

   #36278

   Depositor

   Christian Raetz

   Article

   Chung et al Biochemistry. 2010 May 18;49(19):4126-37

   Insert

   HE-5
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHE-4

   Plasmid

   #36277

   Depositor

   Christian Raetz

   Article

   Chung et al Biochemistry. 2010 May 18;49(19):4126-37

   Insert

   HE-4
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEH-3

   Plasmid

   #36272

   Depositor

   Christian Raetz

   Article

   Chung et al Biochemistry. 2010 May 18;49(19):4126-37

   Insert

   EH-3
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEH-2

   Plasmid

   #36271

   Depositor

   Christian Raetz

   Article

   Chung et al Biochemistry. 2010 May 18;49(19):4126-37

   Insert

   EH-2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEVOL-pAzF

   Plasmid

   #31186

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG

   Depositor

   Peter Schultz

   Article

   Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7

   Insert

   M.j. p-azidohenylalanine RS (2 copies +tRNA)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32T, E107N, D158P, I159L, L162Q, D286R

   Promoter

   Available since

   Dec. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. PVS10

   Plasmid

   #104398

   Purpose

   polycistronic vector for expression of E. coli core RNAP

   Depositor

   Irina Artsimovitch

   Article

   Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

   Insert

   rpoA-rpoB-rpoC-6xHis and rpoZ (rpoA, rpoB, rpoC, rpoZ E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-2(KW)2-mCherry

   Plasmid

   #112738

   Purpose

   Express 2(KW)2-mCherry in E. coli

   Depositor

   Kyubong Jo

   Article

   Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

   Insert

   2(KW)2-mCherry
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBDC

   Plasmid

   #135188

   Purpose

   Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.

   Depositor

   Hongqing Fang

   Article

   Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

   Insert

   CmR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   cat

   Available since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. EcNusGFL

   Plasmid

   #113122

   Purpose

   Expresses full-length E. coli NusG

   Depositor

   James Berger

   Article

   Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

   Insert

   NusG (nusG Escherichia coli)
   

   Use

   Tags

   His6-MBP-N10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMDISI

    Plasmid

    #51653

    Purpose

    E coli genetic modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    spcR
    

    Use

    Genetic modification

    Tags

    Expression

    Mutation

    Promoter

    unknown

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSL4393 (pClpX_Eco)

    Plasmid

    #200907

    Purpose

    Expresses human codon-optimized E. coli ClpX from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    EcoClpX
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTJV1Sc-rpoB

    Plasmid

    #166978

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Inserts

    Ec86 Retron (ECD_00831 )
    lambda bet
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101 and VanCC

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSS9

    Plasmid

    #71655

    Purpose

    Contains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coli

    Depositor

    Ryan Gill

    Article

    Bassalo et al ACS Synth Biol. 2016 Apr 22.

    Insert

    gfpuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7A1

    Available since

    Jan. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Xenopus APC-myc

    Plasmid

    #16686

    Depositor

    Barry Gumbiner

    Article

    Vleminckx et al J Cell Biol. 1997 Jan 27. 136(2):411-20.

    Insert

    APC (apc.L Frog)
    

    Use

    Xenopus expression

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pMH610

    Plasmid

    #131862

    Purpose

    Produces holo-PhyB* in E. coli

    Depositor

    Wilfried Weber

    Article

    Horner et al ACS Synth Biol. 2019 Sep 26. doi: 10.102

    Inserts

    PhyB(1-651)-RGD-His6-Cys
    HO1
    PcyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter-1 and T7 promoter-2

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pXD70LacZ2-Pdadh-LacZ

    Plasmid

    #191618

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p3xFlag-Dam

    Plasmid

    #121909

    Purpose

    Expresses E. coli Dam methyltransferase containing an N-ternimal 3xFlag tag

    Depositor

    Douglas Feldman

    Article

    Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

    Insert

    Dam human codon optimized (dam E. coli)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEB2_mScarletI_mVenusNB-MrkH

    Plasmid

    #182291

    Purpose

    expresses c-di-GMP biosensor in E. coli

    Depositor

    Howard Berg

    Article

    Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

    Insert

    mVenusNB-MrkH
    

    Use

    Tags

    mVenusNB internal fusion to MrkH

    Expression

    Bacterial

    Mutation

    Promoter

    proC

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMDICI

    Plasmid

    #51652

    Purpose

    E coli genetic modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    CmR
    

    Use

    Genetic modification

    Tags

    Expression

    Mutation

    Promoter

    unknown

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pET28a-mCherry-2xEcMTS

    Plasmid

    #133624

    Purpose

    Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    mCherry-2xEcMTS
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMH48

    Plasmid

    #131861

    Purpose

    Produces holo-Cph1* in E. coli

    Depositor

    Wilfried Weber

    Article

    Horner et al ACS Synth Biol. 2019 Sep 26. doi: 10.102

    Inserts

    Cph1(1-514,Y263F)-RGD-His6-Cys
    HO1
    PcyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter-1 and T7 promoter-2

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. P628_SpCas9-RecA

    Plasmid

    #87263

    Purpose

    Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency

    Depositor

    Yonglun Luo

    Article

    Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

    Insert

    RecA
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    no

    Promoter

    CBh

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMAL_dstβL

    Plasmid

    #73805

    Purpose

    Plasmid for selection for insertion and homodimerization

    Depositor

    Sarel Fleishman

    Article

    Elazar et al Elife. 2016 Jan 29;5. pii: e12125. doi:

    Inserts

    CAT Marker
    ToxR
    beta lactamase
    spectomycin resistance
    CLS TM domain
    

    Use

    Tags

    Part Of the dstbl chiemra, Part of the dsTbL chim…

    Expression

    Bacterial

    Mutation

    Promoter

    NaN and ToxR promotor

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

24. pBDK

    Plasmid

    #135190

    Purpose

    Integration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    KanR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cat

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKL001

    Plasmid

    #98917

    Purpose

    Constitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Insert

    CaPR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23118 - Strong Constitutive

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX-HA-GlpG

    Plasmid

    #155135

    Purpose

    Expresses N-terminally GFP- and HA-tagged E. coli GlpG from pGEX 4P-1

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKPY514

    Plasmid

    #62598

    Purpose

    Encodes Thr251Gly-EcPheRS Under IPTG-Inducible (PT5) Control

    Depositor

    David Tirrell

    Article

    Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

    Insert

    Thr251Gly-EcPheRS
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Thr251Gly

    Promoter

    T5

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHL 1075

    Plasmid

    #53004

    Purpose

    pCon:fhlA::mCherry, pLlacO-1:sodB::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    fhlA
    sodB
    

    Use

    Synthetic Biology

    Tags

    GFP and mCherry

    Expression

    Bacterial

    Mutation

    contains −309 to +96 bp relative to start codon a…

    Promoter

    pCon and pLlacO-1

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pET-NtLNtC60αC20C60β

    Plasmid

    #160874

    Purpose

    Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcL, cpn60α, cpn60β, cpn20
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcL: pBAD, chaperones: T7

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDEINO3

    Plasmid

    #179487

    Purpose

    Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

    Depositor

    Bogumil Karas

    Article

    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

    Insert

    TetR/A
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge