We narrowed to 18,622 results for: Met

Showing: 2601 - 2650 of 18622 results

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pPD540 Reporter Template v3

Plasmid

#138750

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation. BsaI sites produce non-palindromic sticky ends.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_sgRNA

Plasmid

#68422

Purpose

Transient expression of a minimal sgRNA targeting the GLuc reporter, in mammalian cells. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

Gluc sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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MLM3587

Plasmid

#43886

Purpose

Activation Domain: VP64; RVD of C-terminal 0.5 TALE repeat: NN (binds G)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator VP64 Backbone

Use

Tale transcriptional activator expression vector

Promoter

EF1alpha

Available Since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

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pXN-FBLWLF-GAL80

Plasmid

#26265

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

GAL80 (GAL80 Budding Yeast)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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EE-SP!gIII

Plasmid

#48667

Purpose

Bacterial SP Cas9 targeting filamentous phage gene III at five protospacers

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
anti-gIII tracrRNA

Use

CRISPR

Promoter

proC and tracrRNA promoter

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pRATIO2251

Plasmid

#141305

Purpose

Gateway cloning compatible ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

Firefly Luciferase or LUC2 and mScarlet-I

Expression

Plant

Promoter

UBQ10 promoter

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pDS247

Plasmid

#34967

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

T406A, T407A, V416I, T–2S

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pDS248

Plasmid

#34968

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

T406A, T407A, V416I

Available Since

March 21, 2012

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNeo3/Cre

Plasmid

#89653

Purpose

Expresses a Neomycin-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Neomycin

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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XW21

Plasmid

#65837

Purpose

XW21 Punc-4c-QS-3'UTR-unc-54 (no SL2-mcherry)

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

Neurospora crassa OR74A repressor (qa-1S)

Expression

Worm

Promoter

Punc-4c

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAP639-2F

Plasmid

#99486

Purpose

3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag

Expression

Bacterial

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ1666

Plasmid

#51485

Purpose

miniMos transposable element with PuroR, MCS cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Expression

Worm

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pAP608-1

Plasmid

#99484

Purpose

H2B::linker::GFP (contain 3 introns)::3XFlag::TEV::Myc::tbb-2 3UTR

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

H2B::linker::GFP with introns::3XFlag::TEV::Myc::tbb-2 3UTR

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP607-2

Plasmid

#99483

Purpose

Myc::TEV::3XFlag::GFP::3XFlag::TEV::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

Myc::TEV::3XFlag::GFP::3XFlag::TEV::Myc

Expression

Bacterial

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pXN-FBLWLF-VP16AD

Plasmid

#26268

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

VP16AD-ZIP+

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV CB6 FFLuc

Plasmid

#35655

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Type

Empty backbone

Use

AAV

Available Since

July 11, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode83

Plasmid

#83760

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode83

Use

Retroviral

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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flGalT-SBP-mCherry_puromycin

Plasmid

#65276

Purpose

GalT reporter only ( no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

galactosidase

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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tdTomato-Cx43-7

Plasmid

#58086

Purpose

Localization: Gap Junctions, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Cx43

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
pXN-FBLWLF-GAL4AD

Plasmid

#26263

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

Gal4AD-Zip+ (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pSC101-MP-mRFP1

Plasmid

#240204

Purpose

Low copy mRFP1 reporter for monitoring metabolite-responsive CRISPRa activity

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

mRFP1

Expression

Bacterial

Promoter

J3-DA9-UP1-MP1

Available Since

Nov. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pColE1-MP-mRFP1

Plasmid

#240203

Purpose

Medium copy mRFP1 reporter for monitoring metabolite-responsive CRISPRa activity

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

mRFP1

Expression

Bacterial

Promoter

J3-DA9-UP1-MP1

Available Since

Nov. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pJM220-PhoA-mNeongreen-J23101-mScarletI

Plasmid

#240201

Purpose

MiniTn7 donor plasmid for constructing a ratiometric reporter for P limitation in Pseudomonas synxantha. A strong Anderson promoter (J23101) drives expression of mScarlet-I

Depositor

Richard Murray

Article

Larsson et al ACS Synth Biol. 2024 Jan 19;13(1):384-39

Inserts

mNeongreen
mScarlet-I

Promoter

J23101 and PphoA

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM220-PphoA-mNeongreen-J23116-mScarletI

Plasmid

#240200

Purpose

MiniTn7 donor plasmid for constructing a ratiometric reporter for P limitation in Pseudomonas synxantha. A weak Anderson promoter (J23116) drives expression of mScarlet-I

Depositor

Richard Murray

Article

Larsson et al ACS Synth Biol. 2024 Jan 19;13(1):384-39

Inserts

mNeongreen
mScarlet-I

Promoter

J23116 and PphoA

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1426

Plasmid

#221573

Purpose

Plasmid containing the Insert 1 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1427

Plasmid

#221574

Purpose

Plasmid containing the Insert 2 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1428

Plasmid

#221575

Purpose

Plasmid containing the Insert 3 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1429

Plasmid

#221576

Purpose

Plasmid containing the Insert 4 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUASTattB-cytoFLARE1.0-TF

Plasmid

#234521

Purpose

Transcriptional reporter for detecting calcium transients in Drosophila larvae (transcription factor)

Depositor

Wenjing Wang

Article

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

ERT2-MK2-hLOV-TEVcs-FLAG-LexA-VP16

Expression

Insect

Available Since

May 1, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAAV-cytoFLARE1.1-TEV

Plasmid

#234518

Purpose

Transcriptional reporter for detecting calcium transients in neuronal cultures (TEVp)

Depositor

Wenjing Wang

Article

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

EGFP-P2A-CaM-V5-TEVp

Use

AAV

Expression

Mammalian

Available Since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGGD_MUTT2A_E

Plasmid

#218899

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

MUTT2A

Use

Synthetic Biology

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MGS0156_pET29b

Plasmid

#215410

Purpose

Expression construct for MGS0156 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

MGS0156

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-LacO-Halo-RGD-STReTCh (Force Inert)

Plasmid

#216710

Purpose

Expresses HaloTag-RGD-STReTCh Force Inert Control in bacteria

Depositor

Alex Dunn

Article

Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

Insert

HaloTag-RGD-STReTCh

Tags

6xHis and HaloTag

Expression

Bacterial

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag Hygro BFP nLAP +0

Plasmid

#194317

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(BFP)

Use

Bacterial cloning vector

Available Since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag Hygro BFP nLAP -1

Plasmid

#194324

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(BFP)

Use

Bacterial cloning vector

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag Hygro BFP nLAP -0

Plasmid

#194323

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(BFP)

Use

Bacterial cloning vector

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag Hygro BFP nLAP -2

Plasmid

#194325

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(BFP)

Use

Bacterial cloning vector

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag Hygro BFP nLAP +1

Plasmid

#194318

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(BFP)

Use

Bacterial cloning vector

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag Hygro BFP nLAP +2

Plasmid

#194319

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(BFP)

Use

Bacterial cloning vector

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ubc-nls-pcp-10xSunTag (SRv1.1)

Plasmid

#185798

Purpose

pcp-10xSunTag component for SunRISER SRv1.1

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

pcp-10xSunTag

Tags

FactorXa site, HA, and NLS

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
ubc-nls-pcp-12xSunTag (SRv1.2)

Plasmid

#185799

Purpose

pcp-12xSunTag component for SunRISER SRv1.2

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

pcp-12xSunTag

Tags

FactorXa site, HA, and NLS

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
His-TEV-GB1 Initial Gly

Plasmid

#128685

Purpose

Expresses GB1 mutant with no Methionine residues

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
CSM160

Plasmid

#105684

Purpose

Plasmid containing an RFP dropout used to construct randomized PAM library

Depositor

Chase Beisel

Article

Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

Insert

RFP Golden Gate dropout

Expression

Bacterial

Promoter

Bba_R0040 TetR Promoter

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD027 Reporter Template v1 (Near Spaced)

Plasmid

#138751

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD152 Reporter Template v2

Plasmid

#138752

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation. BsaI sites produce palindromic sticky ends.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD032 Reporter Template v1 (Mid Spaced)

Plasmid

#138753

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD033 Reporter Template v1 (Far Spaced)

Plasmid

#138754

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EMX1*_sgRNA

Plasmid

#100558

Purpose

Expresses EMX1* sgRNA. Target sequence: (G)GAGTCCGAGCAGAAGAAGAA. Same target sequence as #100555, except with an additional 5' G.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

EMX1 sgRNA

Promoter

U6

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP1071-2

Plasmid

#99502

Purpose

3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3utr::linker::TEV

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3’utr::linker::TEV

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TF101-STTM-miR390

Plasmid

#84812

Purpose

Turning off/down miR390 in Soybean

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM-miR390

Expression

Plant

Promoter

unknown

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 18,622 results for: Met