We narrowed to 49,092 results for: prot

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pBAD/His-miRFP718nano

Plasmid

#195743

Purpose

Monomeric near-infrared fluorescent protein miRFP718nano in bacterial plasmid

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

miRFP718nano

Tags

His

Expression

Bacterial

Promoter

araC

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pmiRFP720-N1

Plasmid

#136560

Purpose

A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

miRFP720

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-neo FlucDM EGFP

Plasmid

#90172

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Mutation

R188Q, R261Q in FLuc

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-neo FlucSM EGFP

Plasmid

#90171

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutation

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Mutation

R188Q in FLuc

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-neo Fluc EGFP

Plasmid

#90170

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSpCas9 (PX165)

Plasmid

#48137

Purpose

Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK1

Plasmid

#122672

Purpose

Expresses mouse PCSK1 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

proprotein convertase subtilisin/kexin type 1 (PCSK1) (Pcsk1 Mouse)

Expression

Mammalian

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK2

Plasmid

#122673

Purpose

Expresses mouse PCSK2 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus proprotein convertase subtilisin/kexin type 2 (Pcsk2) (Pcsk2 Mouse)

Expression

Mammalian

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pA-His6-Tn5

Plasmid

#171871

Purpose

Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

Depositor

Kaiwei Liang

Article

Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

Insert

Protein A and hyperactive Tn5 transposase

Tags

His-tag

Expression

Bacterial

Mutation

WT

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti PGK GFP Puro (w509-5)

Plasmid

#19070

Purpose

3rd gen lentiviral eGFP expression vector, PGK promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pBIG1a

Plasmid

#80611

Purpose

pBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pSF11-wmTagBFP2

Plasmid

#184934

Purpose

Codon optimized blue fluorescent protein mTagBFP2 in C.elegans expresison plasmid

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

mTagBFP2

Expression

Worm

Promoter

tag-168

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLIB

Plasmid

#80610

Purpose

pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Promoter

polyhedrin

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pPHT-PIP2

Plasmid

#60937

Purpose

Fluorescent FPX sensor for PIP2

Depositor

Anne Marie Quinn

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

PIP2 dimerization dependent sensor RA+B monomers

Tags

dimerization dependent fluorescent protein monome…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pH2B-Electra2

Plasmid

#179479

Purpose

Nuclear labeling with blue fluorescent protein Electra2 fused to H2B

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

H2B-Electra2

Tags

Electra2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mCardinal-P2A-GFP

Plasmid

#197167

Purpose

Expresses the protein of mCardinal-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mCardinal-P2A-GFP

Use

AAV

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pmScarlet-I3_C1

Plasmid

#189756

Purpose

C1 Cloning vector for mammalian cells incorporating mScarlet-I3 red fluorescent protein

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I3

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiPGK DEST H2B-iRFP670

Plasmid

#90237

Purpose

Lentiviral vector to express H2B-iRFP670 under PGK promoter

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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secreted BirA-Flag

Plasmid

#64395

Purpose

Flag-tagged biotinylation enzyme for co-expression with bio-proteins

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

BirA (birA E. coli)

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-EGFP-CNA35

Plasmid

#61603

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with EGFP

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

EGFP and CNA35

Tags

His tags

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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