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pHAGE2-TetO-SOX17E57M

Plasmid

#216186

Purpose

Generate lentiviruses encoding for human SOX17E57M (HMG box numbering); for use in pluripotency reprogramming

Depositor

Ralf Jauch

Article

Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

Insert

SOX17 (SOX17 Human)

Use

Lentiviral

Mutation

Glu113Glu (silent mutation); Glu120Glu (silent mu…

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-SOX17E57N

Plasmid

#216187

Purpose

Generate lentiviruses encoding for human SOX17E57N (HMG box numbering); for use in pluripotency reprogramming

Depositor

Ralf Jauch

Article

Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

Insert

SOX17 (SOX17 Human)

Use

Lentiviral

Mutation

Glu113Glu (silent mutation); Glu120Glu (silent mu…

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP-KEAP1 (human)

Plasmid

#236422

Purpose

transient overexpression of KEAP1 in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

Insert

KEAP1 (KEAP1 Human)

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX.(Cyto)iGlucoSnFR.H66A.L276V-mRuby2

Plasmid

#238943

Purpose

Green glucose sensor with mRuby2 fusion, high sensitivity, cytosolic

Depositor

Paul Khavari

Article

Lopez-Pajares et al Cell Stem Cell. 2025 Mar 20:S1934-5909(2

Insert

iGlucoSnFR.L276V.K312A

Use

Lentiviral

Tags

Myc-mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NPM1-TDP43HP

Plasmid

#237669

Purpose

For overexpression of mEGFP-NPM1-TDP43HP

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-TDP43HP (NPM1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_EGFP-NPM1

Plasmid

#237661

Purpose

For integration of EGFP-NPM1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1 (NPM1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_EGFP-NPM1-KS

Plasmid

#237662

Purpose

For integration of EGFP-NPM1-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-KS (NPM1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_EGFP-NPM1-KS-FtoA

Plasmid

#237664

Purpose

For integration of EGFP-NPM1-KS-FtoA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-KS-FtoA (NPM1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_EGFP-NPM1-KS-0F

Plasmid

#237665

Purpose

For integration of EGFP-NPM1-KS-0F

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-KS-0F (NPM1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NPM1-KS

Plasmid

#237668

Purpose

For overexpression of mEGFP-NPM1-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-KS (NPM1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_EGFP-NPM1-KS-FtoG

Plasmid

#237663

Purpose

For integration of EGFP-NPM1-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-KS-FtoG (NPM1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-FOXA1-T2A-miRFP670

Plasmid

#182335

Purpose

Expresses human FOXA1 and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Forkhead Box A1 (FOXA1 Human)

Tags

T2A-miRFP670

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-Nluc-_(PLRSKK)-CA1-19

Plasmid

#232657

Purpose

Nluc-tagged modified CG_ in mammalian cells; lectin-captured luciferase reporter assay

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

CGA (CGA Human)

Use

Luciferase

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pmNeon Green-N1-ARF3-R

Plasmid

#202431

Purpose

Mammalian expression vector containing pmNeonGreen-tagged ARF3

Depositor

David Bryant

Article

Sandilands et al J Cell Biol. 2023 Apr 3;222(4):e20220611

Insert

ARF3 (ARF3 Human)

Use

Lentiviral

Tags

mNeonGreen

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgABCC4_1

Plasmid

#217433

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 1 targeting ABCC4 (ABCC4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgABCC4_3

Plasmid

#217434

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 3 targeting ABCC4 (ABCC4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKS732A/S910A

Plasmid

#221642

Purpose

This retroviral plasmid expresses a double-mutated cDNA (dominant negative: S732A/S910A) of human PTK2

Depositor

Georgia Konstantinidou

Article

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (S732A/S910A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK double mutation S732A/S910A (dominant negativ…

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKP712/713A

Plasmid

#216544

Purpose

This retroviral plasmid expresses a mutated cDNA (dominant negative: P712/713A) of human PTK2

Depositor

Georgia Konstantinidou

Article

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (P712/713A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK dominant negative mutation P712/713A

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKR177/178A

Plasmid

#216543

Purpose

This retroviral plasmid expresses a mutated cDNA (dominant negative: R177/178A) of human PTK2

Depositor

Georgia Konstantinidou

Article

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (R177/178A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK dominant negative mutation R177/178A

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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lentiGuide-puro_Panc480-MT7

Plasmid

#200941

Purpose

Multiplexed CRISPR array expressing 7 sgRNA in a lentiviral backbone

Depositor

James Eshleman

Article

Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

Insert

multiplexed sgRNA array

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6, EF-1a

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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