We narrowed to 12,292 results for: BASE

1. pmCherry

   Plasmid

   #165828

   Purpose

   mCherry red fluorescent protein CDS

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pT2/Onc

   Plasmid

   #21360

   Depositor

   David Largaespada

   Article

   Collier et al Nature. 2005 Jul 14. 436(7048):272-6.

   Insert

   Sleeping Beauty Transposon with splice acceptors and murine stem cell virus LTR
   

   Use

   Transposon

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLA230

   Plasmid

   #11724

   Depositor

   Lawrence Loeb

   Article

   Shinkai et al J Biol Chem. 2001 Dec 14. 276(50):46759-

   Insert

   b-lactamase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GAA for Glu26 changed to TAA (premature stop codo…

   Promoter

   Available since

   Aug. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFLuc190UGA

   Plasmid

   #41046

   Depositor

   James Inglese

   Article

   Auld et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

   Insert

   Firefly luciferase 190UGA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   in-frame UGA stop codon was created at codon 190

   Promoter

   CMV

   Available since

   Nov. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pET28b-6His-AcpS

   Plasmid

   #145376

   Purpose

   Expression of E. coli AcpS

   Depositor

   Robert Tomko

   Article

   Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

   Insert

   AcpS (acpP E. coli)
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pBAD-HaloGFP-Na2.4

   Plasmid

   #228470

   Purpose

   Bacterial expression of green fluorescent sodium ion indicator HaloGFP-Na2.4

   Depositor

   Robert Campbell

   Article

   Takeuchi et al RSC Chem Biol. 2024 Dec 3. doi: 10.1039/

   Insert

   HaloGFP-Na2.4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJET1.2-STOP-dsRed

   Plasmid

   #60944

   Purpose

   Drosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, flanked attP. section marker, assemble with homology arms to make Donor plasmid for CRISPR.

   Depositor

   Frank Schnorrer

   Article

   Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

   Insert

   dsRED
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   3XP3

   Available since

   Nov. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7sgRNA

   Plasmid

   #111820

   Purpose

   Expresses sgRNA in T. brucei cells

   Depositor

   David Horn

   Article

   Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p663-UBC-miniTurbo-V5-TP53RK_IDG-K

   Plasmid

   #170589

   Purpose

   Gateway destination clone of TP53RK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-TP53RK (TP53RK Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRISPaint-3xflag-PuroR

    Plasmid

    #80973

    Purpose

    CRISPaint gene tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    3xflag tag
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKA-142

    Plasmid

    #79835

    Purpose

    Expresses PpsR2-mVenus-CAAX under CMVd1 promoter

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

    Insert

    PpsR2-mVenus-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    RpPpsR2 cysteine 439 changed to serine

    Promoter

    CMVd1

    Available since

    Aug. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAS9-BFP empty

    Plasmid

    #80976

    Purpose

    Cas9, TagBFP, and sgRNA expression

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pICH-roGFP2-synORP1

    Plasmid

    #191680

    Purpose

    Ti plasmid pICH86988 harbouring roGFP2-synORP1 for H2O2 detection in plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    rredox(roGFP2-synORP1
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PMT-mKate2

    Plasmid

    #203785

    Purpose

    Brightness calibration

    Depositor

    Thorsten Wohland

    Article

    Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

    Insert

    PMT-mKate2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLQ11353

    Plasmid

    #183098

    Purpose

    pHR-CAG-sfGFP* ABE_3-WPRE

    Depositor

    Stanley Qi

    Article

    Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

    Insert

    GFP*
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSARSF53-110

    Plasmid

    #61813

    Purpose

    TvoVMAN11 intein

    Depositor

    Hideo Iwai

    Article

    Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

    Insert

    TvoVMAN11 intein
    

    Use

    Tags

    H6-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSABAD14-98

    Plasmid

    #61827

    Purpose

    TvoVMAdN11 intein

    Depositor

    Hideo Iwai

    Article

    Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

    Insert

    TvoVMAdN11 intein
    

    Use

    Tags

    GB1-H6

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDmVasa

    Plasmid

    #165783

    Purpose

    Drosohphila vasa germline promoter and 5'UTR

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Vasa promoter
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pgRNA-R-gfp

    Plasmid

    #220927

    Purpose

    Expresses gfp using J23119 promoter and pgRNA_AT backbone

    Depositor

    Andrea Puhar

    Article

    Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

    Insert

    superfolder GFP
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMAL-BRT17

    Plasmid

    #45358

    Depositor

    Scott Banta

    Article

    Shur et al Biotechniques. 2013 Apr;54(4):197-206. d

    Insert

    17 Repeat Beta Roll Tag
    

    Use

    Tags

    Maltose Binding Protein (MBP)

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. VV063: 1xCox8 - superecliptic pHluorin in fck

    Plasmid

    #58500

    Purpose

    Expresses the pH sensor superecliptic pHluorin in the mitochondria of mammalian cells

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    1xCox8 - Superecliptic pHluorin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    a-CamKII

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMRE-Himar-142

    Plasmid

    #185931

    Purpose

    Himar transposon delivery plasmid that delivers a green fluorescent sGFP2 protein gene

    Depositor

    Mitja Remus-Emsermann

    Article

    Stocks et al Bacteria 2022, 1(1), 56-65

    Insert

    sGFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTHSSd_41

    Plasmid

    #59964

    Purpose

    High full length T7 RNAP* expression

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

    Insert

    T7* RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    PJ23105

    Available since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCRISPaint-SBP

    Plasmid

    #67173

    Purpose

    CRISPaint universal donor for streptavidin binding peptide fusion tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    SBP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCOLA-T7-WspR:D70E

    Plasmid

    #79164

    Purpose

    Expresses constitutively active WspR D70E under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    WspR (wspR P. areuginosa)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D70E

    Promoter

    T7

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p3xP3-EGFP.vas-int.NLS

    Plasmid

    #60948

    Purpose

    Drosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR.

    Depositor

    Frank Schnorrer

    Article

    Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

    Insert

    vas-phiC31 integrase.NLS
    

    Use

    Rmce

    Tags

    Expression

    Mutation

    Promoter

    vasa

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pTPGI_dCas9

    Plasmid

    #64279

    Purpose

    encodes yeast-optimized dCas9 synthetic transcription factor

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    Yeast-optimized dCas9
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    D10A & H840A relative to S. pyogenes Cas9

    Promoter

    pTPGI

    Available since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFC31

    Plasmid

    #165845

    Purpose

    _C31 integrase CDS

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    _C31 integrase
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJRK261

    Plasmid

    #173754

    Purpose

    CeLINC plasmid

    Depositor

    Mike Boxem

    Article

    Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

    Insert

    (empty)::CRY2(olig)::VHH(GFP)::T2A::CIBN-3xFLAG::MP::unc-54 3' UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAS9-mCherry-TUBB

    Plasmid

    #66942

    Purpose

    CRISPaint target selector TUBB

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA TUBB
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCOLA-T7-WspR

    Plasmid

    #79162

    Purpose

    Expresses WspR WT under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    WspR (wspR P. areuginosa)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. VV064: 1xCox8 - ratiometric pHluorin in fck

    Plasmid

    #58502

    Purpose

    Expresses the pH sensor ratiometric pHluorin in the mitochondria of mammalian cells

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    1xCox8 - Ratiometric pHluorin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    a-CamKII

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSLQ11350

    Plasmid

    #183207

    Purpose

    PB-TRE3G-BFP-Triplex-crGFP*ABE3-SV40pA

    Depositor

    Stanley Qi

    Article

    Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

    Insert

    Cas12 Guide (crGFP*)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMOTag-glmS-4H

    Plasmid

    #106378

    Purpose

    Can be used as a PCR template to amplify the glmS-HA fragment for gene specific tagging and regulation

    Depositor

    Roberto Docampo

    Article

    Cruz-Bustos et al J Eukaryot Microbiol. 2017 Dec 19. doi:

    Insert

    GlmS-3xHA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 2, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. BBa_J72114-BBa_J72104

    Plasmid

    #40783

    Depositor

    Christopher Anderson

    Article

    Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

    Insert

    RhaRS + pRhaB + LuxCDABE, VioABCE, LacZ
    

    Use

    Luciferase and Synthetic Biology; Phagemid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. p667-UBC-AAK1-V5_IDG-K

    Plasmid

    #135257

    Purpose

    Gateway destination clone of AAK1 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    AAK1-V5 (AAK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. LeGo-Renilla-BLAST

    Plasmid

    #122276

    Purpose

    Lentiviral vector for stable expression of Renilla luciferase.

    Depositor

    Demián Cazalla

    Article

    Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

    Insert

    Renilla luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PMT-mEGFP

    Plasmid

    #203777

    Purpose

    Brightness calibration

    Depositor

    Thorsten Wohland

    Article

    Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

    Insert

    PMT-mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PMT-mTurquoise2_2

    Plasmid

    #203776

    Purpose

    Brightness calibration

    Depositor

    Thorsten Wohland

    Article

    Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

    Insert

    PMT-mTurquoise2 dimer
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PMT-mTurquoise2

    Plasmid

    #203775

    Purpose

    Brightness calibration

    Depositor

    Thorsten Wohland

    Article

    Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

    Insert

    PMT-mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge