161,805 results

1. pCAG-TetON-3G

   Plasmid

   #96963

   Purpose

   Mammalian expression of Clontech rtTA driven by human beta-actin promoter

   Depositor

   Elena Cattaneo

   Article

   Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUC19-eGFP-P2A-dTAG-Ing3

   Plasmid

   #170754

   Purpose

   Donor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locus

   Depositor

   Kristian Helin

   Article

   Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

   Insert

   eGFP-P2A-2xHA-dTAG
   

   Use

   Mouse Targeting

   Tags

   eGFP-P2A-2xHA-dTAG

   Expression

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   none

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pICE-RNaseHI-D10R-E48R-NLS-mCherry

   Plasmid

   #60367

   Purpose

   Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

   Insert

   RNase HI-D10R-E48R
   

   Use

   Tags

   NLS from SV40 T antigen and mCherry

   Expression

   Mammalian

   Mutation

   D10R+E48R; catalyticaly inactive

   Promoter

   CMV-tet

   Available since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. dead Sa Cas9

   Plasmid

   #138162

   Purpose

   For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   dSaCas9
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   D10A + N580A

   Promoter

   CMV

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPOTv7-blast-10FLAG

   Plasmid

   #181940

   Purpose

   Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

   Depositor

   Samuel Dean

   Article

   Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

   Insert

   10xFLAG
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   read through

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mCherry-Alpha-Actin-12

   Plasmid

   #54971

   Purpose

   Localization: Actin, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Alpha-Actin (ACTA2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSSA-RPG

   Plasmid

   #85932

   Purpose

   Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells

   Depositor

   Zhiying Zhang

   Article

   Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-

   Insert

   pPB-CMV-DsRed-polyA-CAG-SSA. Puro-T2A-EGFP
   

   Use

   Dual-reporter surrogate

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p3E-Rac1

   Plasmid

   #109581

   Purpose

   Multisite gateway vector for 3' tagging with Rac1

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Rac1 (rac1a Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FL-fibulin-3-V5 pcDNA4

   Plasmid

   #29701

   Depositor

   Mariano Viapiano

   Article

   Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

   Insert

   EFEMP1 (EFEMP1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

10. M_ST1n_VPR

    Plasmid

    #63799

    Purpose

    ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    ST1-dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKOV

    Plasmid

    #25769

    Depositor

    George Church

    Article

    Link et al J Bacteriol. 1997 Oct . 179(20):6228-37.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRK6-HA-TAK1

    Plasmid

    #44160

    Depositor

    Xin Lin

    Article

    Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

    Insert

    Tak1 (Map3k7 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

13. WWP-Luc (p21/WAF1 promoter)

    Plasmid

    #16451

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

    Insert

    WAF1 promoter (CDKN1A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA 3.3 HA-hTSPAN12

    Plasmid

    #115784

    Purpose

    Expresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    TSPAN12 (TSPAN12 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    multiple silent mutations introduce restriction s…

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CFP_Rad_pcDNA4_HisMaxC

    Plasmid

    #41654

    Depositor

    Henry Colecraft

    Article

    Yang et al PLoS One. 2012;7(5):e37079. Epub 2012 Ma

    Insert

    Rad, GTP binding proteins (RGK) (Rrad Mouse)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBI-EGFP-MnSOD

    Plasmid

    #16612

    Depositor

    Bert Vogelstein

    Article

    Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

    Insert

    MnSOD (SOD2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LDB-14-p65

    Plasmid

    #158985

    Purpose

    Expresses LDB-14 fused with a C-terminal p65 transcriptional activation domain in mammalian cells

    Depositor

    Liangcai Gu

    Article

    Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

    Insert

    LDB-14-p65
    

    Use

    Tags

    V5 tag , 6xHis tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBFv-U6.2B

    Plasmid

    #138401

    Purpose

    gRNA expression vector; Can be combined with pBFv-U6.2 to generate double-gRNA expression vector

    Depositor

    Shu Kondo

    Article

    Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

    Insert

    U6-2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6-2

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PH-PLCD1(R40L)-GFP

    Plasmid

    #51408

    Purpose

    Non-PI(4,5)P2 binding control for PH-PLCD1-GFP

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    PH-PLCD1 (aa 1–170) (PLCD1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Arginine 40 mutated to leucine

    Promoter

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

20. mCherry-C1-TGAT

    Plasmid

    #84334

    Purpose

    Expresses TGAT tagged with mCherry

    Depositor

    Dorus Gadella

    Article

    van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

    Insert

    TGAT (TRIO Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Dec. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge