19,770 results

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pHR-FKBP:mCherry-Rab11a

Plasmid

#72902

Purpose

Recycling endosome-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Rab11a (RAB11A Human)

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

fragment a.a. 2-216

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-mEGFP

Plasmid

#175293

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with mEGFP

Tags

mEGFP

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pHCMV_Bless

Plasmid

#207255

Purpose

Mammalian expression of Baboon Endogenous Retrovirus env protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

BaboonEndogenousRetrovirus_Rless_env

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas

Plasmid

#106171

Purpose

cre mediated red to green shift

Depositor

Ewa Snaar-Jagalska

Article

Heitzer et al Sci Rep. 2019 Mar 11;9(1):4096. doi: 10.

Inserts

dsRED-express2
eGFP

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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CuGFP-ATG8(416)

Plasmid

#49423

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the CUP1 promoter.

Depositor

Daniel Klionsky

Article

Wang et al J Biol Chem. 2012 Nov 2;287(45):37964-72

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Tags

GFP

Expression

Bacterial and Yeast

Promoter

CUP1

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag TGIF

Plasmid

#14047

Depositor

Joan Massague

Article

Wotton et al J Biol Chem. 1999 Dec 24. 274(52):37105-

Insert

TGIF (TGIF1 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pCHNFI-X2

Plasmid

#31402

Depositor

Richard Gronostajski

Article

Chaudhry et al J Biol Chem. 1998 Jul 17. 273(29):18538-

Insert

Nuclear Factor IX isoform 2 (Nfix Mouse)

Tags

NA

Expression

Mammalian

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

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Lck-mScarlet-I

Plasmid

#98821

Purpose

In vivo visualization of the plasma membrane (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Lck

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pLifeAct-miRFP670nano

Plasmid

#127434

Purpose

F-Actin labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

LifeAct-miRFP670nano

Tags

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pNW-sfGFP(SPS6)

Plasmid

#133814

Purpose

Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides.

Depositor

Oscar Kuipers

Article

Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

Insert

sfGFP(SPS6)

Use

E.coli/bacillus spp. shuttle vector

Expression

Bacterial

Promoter

Ppta

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-NE2m

Plasmid

#208686

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m in neurons

Depositor

Yulong Li

Article

Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2m

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHIV-Luc-ZsGreen

Plasmid

#39196

Depositor

Bryan Welm

Inserts

Firefly Luciferase Luc2P
ZsGreen

Use

Lentiviral

Expression

Mammalian

Promoter

EF1-alpha and EF1-alpha

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pSIRV-NF-kB-eCFP

Plasmid

#118094

Purpose

Flourescent reporter for NF-kB activity

Depositor

Peter Steinberger

Article

Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

Insert

eCFP

Use

Retroviral

Promoter

minimal promoter with NF-kB responsive element

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-mCherry

Plasmid

#36084

Purpose

3rd generation lentiviral plasmid expressing mCherry

Depositor

Pantelis Tsoulfas

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

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pRSETB/mStayGold(c4)

Plasmid

#212018

Purpose

For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

mStayGold(c4)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pBOB-EF1-FastFUCCI-Puro

Plasmid

#86849

Purpose

3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection

Depositor

Kevin Brindle, Duncan Jodrell

Article

Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164

Inserts

mKO2-hCDT1(30-120)
mAG-hGEM(1-110)
pac (puromycin N-acetyl-transferase)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1 and PGK

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-RARE-luciferase

Plasmid

#13458

Depositor

T. Michael Underhill

Article

Hoffman et al J Cell Biol. 2006 Jul 3. 174(1):101-13.

Insert

Retinoic Acid Receptor Response Element

Use

Luciferase

Available Since

Nov. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pAG/MNase

Plasmid

#123461

Purpose

Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.

Depositor

Steven Henikoff

Article

Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1

Insert

Protein A-Protein G-Micrococcal Nuclease

Tags

6xHIS and HA

Expression

Bacterial

Promoter

Lac

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_ACh3.0

Plasmid

#121922

Purpose

express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0

Depositor

Yulong Li

Article

Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

Has Service

AAV9

Insert

GPCR activation based ACh sensor GRAB-ACh3.0

Use

AAV

Promoter

hSyn

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pYD1

Plasmid

#73447

Purpose

expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells

Depositor

Dane Wittrup

Article

Kieke et al Protein Eng. 1997 Nov;10(11):1303-10.

Type

Empty backbone

Tags

6xHis, AGA2, T7 peptide, V5 tag, and Xpress tag

Expression

Yeast

Promoter

GAL1

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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