20,095 results

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pDEST40-2XHA-wtSrc

Plasmid

#140294

Purpose

Expresses HA-tagged wild-type human Src in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src wildtype (SRC Human)

Tags

2X HA

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCMU-NUCr

Plasmid

#61168

Purpose

Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

NLS-mCherry-GUS

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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GRM5-Tango

Plasmid

#66390

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GRM5 (GRM5 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pJ3 SHP1 WT

Plasmid

#8572

Depositor

Ben Neel

Insert

SHP1 (PTPN6 Human)

Expression

Mammalian

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only
7TFP CDH1 reporter

Plasmid

#91704

Purpose

lentiviral luciferase reporter containing CDH1 promoter

Depositor

Bob Weinberg

Article

Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

Insert

CDH1 promoter (CDH1 Human)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

CDH1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSBtet-BH

Plasmid

#60499

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and hygromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

TCE & RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKD1

Plasmid

#23500

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKD1 (PRKD1 Human)

Use

Gateway donor vector

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pQE60-EFTs-His

Plasmid

#124128

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Elongation factor Ts (tsf Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEBTet_NADP-Snifit-cyto

Plasmid

#113917

Purpose

Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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psigma2-EGFP

Plasmid

#53610

Purpose

Expresses sigma2 of adaptor protein of AP2

Depositor

Tom Kirchhausen

Article

Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

Insert

sigma2 (Ap2s1 Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST-V1-ORF

Plasmid

#73635

Purpose

Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 1 (V1).

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 1 (V1)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pINDUCER20-α-Syn

Plasmid

#92200

Purpose

Retroviral overexpression vector (doxycycline-inducible) for α-Syn

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

SNCA (SNCA Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-bleo IGF-1R

Plasmid

#11212

Depositor

Ronald Kahn

Article

Entingh-Pearsall et al J Biol Chem. 2004 Sep 3. 279(36):38016-2

Insert

IGF-1R (IGF1R Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp8 C360A

Plasmid

#11818

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 8 catalytic mutant (CASP8 Human)

Tags

HA

Expression

Mammalian

Mutation

C360A

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

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pET-53-RtcB

Plasmid

#51282

Purpose

E. coli expression vector for E. coli RtcB RNA ligase

Depositor

Jay Hesselberth

Article

Peach et al Nucleic Acids Res. 2015 May 22. pii: gkv

Insert

E. coli RtcB

Tags

6xHis

Expression

Bacterial

Promoter

T7 RNA Polymerase

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAG425GPD-ccdB-EGFP

Plasmid

#14202

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pFUGW mCherry-KASH

Plasmid

#131505

Purpose

mCherry_KASH epression under hUBC promotor; serves as backbone for ORANGE lenti knock-in

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

mCherry_KASH

Use

Lentiviral

Expression

Mammalian

Promoter

hUBC

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR4 no ccDB (686-1)

Plasmid

#17424

Purpose

Gateway entry vector, no ccDB gene

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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PJ-INPP5E

Plasmid

#38001

Depositor

Robin Irvine

Article

Hammond et al Science. 2012 Jun 21.

Inserts

FKBP1A (FKBP1A Human)
SAC1 (SAC1 Budding Yeast)
INPP5E (INPP5E Human)

Tags

mRFP

Expression

Mammalian

Mutation

Cys 779 mutated to Ser

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3-PAX3-FOXO1/FKHR

Plasmid

#115526

Purpose

Mammalian expression construct

Depositor

Martine Roussel

Article

Lam et al Mol Cell Biol. 1999 Jan;19(1):594-601.

Insert

Pax3-FOXO1/FKHR (FOXO1, Pax3 Mouse, Human)

Expression

Mammalian

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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