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pLJM1-Lyso-FLAG-GFP-Sac1-Cat-WT

Plasmid

#134645

Purpose

lysosome-targeted Sac1 catalytic domain wild type

Depositor

Roberto Zoncu

Article

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

Sac1 catalytic domain (77-520 aa) (SACM1L Human)

Use

Lentiviral

Tags

FLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…

Expression

Mammalian

Mutation

contains only amino acids 77-520 (Please see depo…

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CS

Plasmid

#134653

Purpose

lysosome-targeted Sac1 catalytic domain C389S

Depositor

Roberto Zoncu

Article

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

Sac1 catalytic domain (77-520 aa) CS mutant (SACM1L Human)

Use

Lentiviral

Tags

FLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…

Expression

Mammalian

Mutation

C389S and contains only amino acids 77-520 (Pleas…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pT3-EF1A-mTert

Plasmid

#162555

Purpose

Overexpression of Tert

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

Overexpression of Tert (Tert Mouse)

Expression

Mammalian

Mutation

None

Promoter

EF1A

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-Jaws-GFP-ER2

Plasmid

#65016

Purpose

AAV-mediated expression of Jaws-ER2 under the human synapsin promoter

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-GFP-ER2

Use

AAV

Tags

ER2 and GFP

Expression

Mammalian

Mutation

K200R W214F

Promoter

human synapsin

Available Since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-HyPer7-MEM

Plasmid

#136465

Purpose

Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Farnselation tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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GLI1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176968

Purpose

Mammalian expression vector encoding GLI1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

GLI1 (GLI1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-IKBalpha-mut (super repressor)

Plasmid

#15291

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

inhibitor of Kappa B alpha (NFKBIA Human)

Use

Retroviral

Expression

Mammalian

Mutation

S32A/S36A: “super-repressor” allele (resistant to…

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pEGFP-GFP11-Clathrin light chain

Plasmid

#70217

Purpose

Expresses GFP11-clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP11-Clathrin light chain (CLTA Human)

Tags

GFP11

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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CMV_R-PTEN

Plasmid

#227433

Purpose

Expresses the R-PTEN sensor under a CMV promoter

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

R-PTEN (Pten Rat)

Tags

mCyRFP2, mMaroon

Expression

Mammalian

Mutation

R14G

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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BII-CMV-AfmW3A

Plasmid

#133402

Purpose

Piggybac vector for constitutive expression of Afamin + Wnt3a for production of conditioned media in HEK293T cells

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

Afamin and Wnt3a (Afm, Wnt3a Mouse)

Expression

Mammalian

Mutation

N/A

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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MS2-P65-HSF1_GFP

Plasmid

#61423

Purpose

Expresses the MS2-P65-HSF1 activator helper complex with a 2A GFP. 3rd generation lentiviral vector.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Insert

MS2(N55K)-P65-HSF1_2A_GFP (HSF1 Human, Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1A

Available Since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAAVS1P-iCAG.copGFP

Plasmid

#66577

Purpose

AAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-AAVS1 TALENs

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

copGFP

Expression

Mammalian

Promoter

CAG

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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PL-5LTR-RGR(DMD#1)-AmCyan-A

Plasmid

#138482

Purpose

Expresses sgRNA targeting human DMD (Dystrophin) for packaging into NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

Ribozyme-flanked gRNA and AmCyan

Use

CRISPR

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLX302_FOXA1-V5

Plasmid

#70090

Purpose

Expression of human FOXA1 with C-terminal V5 tag, puromycin selection marker, 3rd generation lentiviral vector

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PDi-CRISPRn

Plasmid

#73500

Purpose

Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

Cas9
rtTA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Promoter

CAG and TRE

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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CMV_G-PTEN

Plasmid

#227432

Purpose

Expresses the G-PTEN sensor under a CMV promoter

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

G-PTEN (Pten Rat)

Tags

mEGFP and sREACh

Expression

Mammalian

Mutation

R14G

Promoter

CMV

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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PEA1-Puro

Plasmid

#171991

Purpose

Delivers all prime editing nickase components in a single, puromycin selectable plasmid

Depositor

Paul Thomas

Article

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)

Expression

Mammalian

Promoter

CMV for Cas9, U6 for gRNAs

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-human Igf2bp2-IRES-GFP

Plasmid

#91890

Purpose

Retroviral expression of human Igfbp2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Igf2bp2 (IGF2BP2 Human)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

Plasmid

#183410

Purpose

piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells

Depositor

Azim Surani

Article

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Insert

KRAB-dCas9-ecDHFR

Use

CRISPR and Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE3G

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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CTCF-mAC donor (Neo)

Plasmid

#140645

Purpose

CTCF tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CTCF (CTCF Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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