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We narrowed to 5,536 results for: Chia

Showing: 2801 - 2850 of 5536 results

  1. pYG205

    Plasmid
    #200836
    Purpose
    Transcribes rapZ from the arabinose-inducible PBAD promoter; RapZ directs RNase E to cleave fusion RNAs transcribed from pYG215 or derivatives
    Depositor
    Boris Görke
    Article
    Schilder et al Nucleic Acids Res. 2023 Mar 29:gkad226.
    Insert
    rapZ (rapZ Escherichia coli str. K-12 substr. MG1655)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P-BAD
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    200836-plasmid-map-sequence-id-404931

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  2. pTP-PCR C1

    Plasmid
    #189618
    Purpose
    Cloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Bogumil Karas
    Article
    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:
    Insert
    Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Minor additional mutations described in manuscript
    Promoter
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    189618-plasmid-map-sequence-id-401652

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  3. pCAGGS-C-TEV-HAT

    Plasmid
    #202597
    Purpose
    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Use
    Tags
    TEV cleavable site (ENLYFQG) and poly-histidine a…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202597-plasmid-map-sequence-id-399552

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  4. pCAGGS-N-TEV-HAT

    Plasmid
    #202598
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Use
    Tags
    TEV cleavable site (ENLYFQG) and poly-histidine a…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202598-plasmid-map-sequence-id-399468

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  7. pTP-PCR C4

    Plasmid
    #189621
    Purpose
    Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Bogumil Karas
    Article
    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:
    Insert
    Reduced Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Repeat region removed, and minor additional mutat…
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    189621-plasmid-map-sequence-id-379021

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  8. pTP-PCR C2

    Plasmid
    #189619
    Purpose
    Cloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Bogumil Karas
    Article
    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:
    Insert
    Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Minor additional mutations described in manuscript
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    189619-plasmid-map-sequence-id-379034

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  10. NeuA.pJL1

    Plasmid
    #199115
    Purpose
    In vitro expression of N-acylneuraminate cytodiylyltransfease (NeuA), which conjugates sialic acid and CTP to make activated sugar donor, CMP-sialic acid (see CSTI.PJL1 and PdST6.PJL1)
    Depositor
    Michael Jewett
    Article
    Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102
    Insert
    N-acylneuraminate cytodiylyltransfease (NeuA)
    Use
    Tags
    Strep-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199115-plasmid-map-sequence-id-390797

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  16. pDR111_mannose_Bgal NLS

    Plasmid
    #188398
    Purpose
    Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Insert
    lacZ
    Use
    Tags
    PhoD secretion peptide and SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    D-mannose
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188398-plasmid-map-sequence-id-370473

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  19. pBN216

    Plasmid
    #167963
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y713E, Y715E, Y717E, Y718E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167963-plasmid-map-sequence-id-344805

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  20. pBN217

    Plasmid
    #167964
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y708E, Y713E, Y715E, Y717E, Y718E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167964-plasmid-map-sequence-id-344780

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  21. pBN262

    Plasmid
    #167967
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y708E, Y713E, Y715E, Y717E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167967-plasmid-map-sequence-id-344775

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  22. pBRC901

    Plasmid
    #167948
    Purpose
    express wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167948-plasmid-map-sequence-id-344794

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  23. pBN202

    Plasmid
    #167950
    Purpose
    express E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M, Y713E, Y715E, Y717E, Y718E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167950-plasmid-map-sequence-id-344771

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  25. pKP1693

    Plasmid
    #167595
    Purpose
    Express FepA with His6 tag between residues 393 and 394 of the mature amino acid sequence . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Gresock et al J Bacteriol. 2017 Apr 25;199(10). pii: J
    Insert
    FepA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    His6 tag inserted after residue 393 in the mature…
    Promoter
    Available since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167595-plasmid-map-sequence-id-332904

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  27. pKB238

    Plasmid
    #170033
    Purpose
    Encodes LacI-controlled expression of surface-displayed NPY and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    Neuropeptide Y (NPY Human, Escherichia coli, Synthetic)
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170033-plasmid-map-sequence-id-336781

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  28. pKB235

    Plasmid
    #170024
    Purpose
    Encodes LacI-controlled expression of surface-displayed CCL20 and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    CCL20 (CCL20 Human, Escherichia coli, Synthetic)
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170024-plasmid-map-sequence-id-336757

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  29. AlkB-D135G

    Plasmid
    #160434
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Glycine; deleted the…
    Promoter
    T7
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160434-plasmid-map-sequence-id-318954

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  30. pCDF-NtXNtS1HR1AtR2NtB2

    Plasmid
    #160890
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    PT7-Nt-rbcS-S1H
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-SH1: T7, chaperones: T13
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160890-plasmid-map-sequence-id-318865

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  31. pCDF-NtXNtT4aR1AtR2NtB2

    Plasmid
    #160887
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    PT7-Nt-rbcS-T4a
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-T4a: T7, chaperones: T10
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160887-plasmid-map-sequence-id-318851

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  32. SMT215

    Plasmid
    #134818
    Purpose
    SMT205 with DHFR-FLAG-tag, western blot
    Depositor
    Tanja Kortemme
    Article
    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1
    Inserts
    Dihydrofolate reductase
    Thymidylate synthase
    Use
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Silent mutations to eliminate internal restrictio…
    Promoter
    T7 and TET
    Available since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    134818-plasmid-map-sequence-id-313944

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  35. SMT201

    Plasmid
    #134816
    Purpose
    SMT101 with TET promoter for TYMS, in vivo assays
    Depositor
    Tanja Kortemme
    Article
    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1
    Inserts
    Dihydrofolate reductase
    Thymidylate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Silent mutations to eliminate internal restrictio…
    Promoter
    T7 and TET
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134816-plasmid-map-sequence-id-274005

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  36. pC0.007

    Plasmid
    #119671
    Purpose
    Level 0 part. Promoter, cpc560 promoter, extended spacer region, downstream TFB site region duplicated.
    Depositor
    Alistair McCormick
    Article
    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do
    Insert
    Pcpc560-Dx2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    bp 89 T to C, bp 187 C to A, Duplicated downstrea…
    Promoter
    Available since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    119671-plasmid-map-sequence-id-237673

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  42. pAL832

    Plasmid
    #119305
    Purpose
    precusor outer membrane protein A, ompA, truncated after amino acid 176
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precursor outer membrane protein A, ompA, truncated after amino acid 176 including with mutations T95C and I153C
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    truncated after amino acid 176, mutations Threoni…
    Promoter
    lac
    Available since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119305-plasmid-map-sequence-id-232460

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  43. pAL800

    Plasmid
    #119306
    Purpose
    precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    amino acids 1 through 171 deleted, and mutatioms …
    Promoter
    lac
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119306-plasmid-map-sequence-id-232254

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  44. pAL928

    Plasmid
    #119853
    Purpose
    precusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427C
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    Cysteine 169 to Serine, Cysteine 179 to Serine, C…
    Promoter
    T7
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119853-plasmid-map-sequence-id-232052

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Showing: 2801 - 2850 of 5536 results