We narrowed to 17,355 results for: URE

1. pCDB185

   Plasmid

   #80688

   Purpose

   for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEH_04

   Depositor

   David Baker

   Article

   Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

   Insert

   gEEH_04
   

   Use

   Tags

   OsmY-10His-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDB285

   Plasmid

   #80687

   Purpose

   for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEEH_04

   Depositor

   David Baker

   Article

   Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

   Insert

   gEEEH_04
   

   Use

   Tags

   OsmY-10His-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant

   Plasmid

   #101213

   Purpose

   Bacterial expression plasmid for SpCas9 + K855A variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K855A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K855A

   Promoter

   T7

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers

   Plasmid

   #50421

   Purpose

   pTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.

   Depositor

   Andrew Scharenberg

   Article

   Boissel et al Nucleic Acids Res. 2013 Nov 26.

   Type

   Empty backbone

   Use

   Lentiviral and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   May 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKMW306_U6-sgRNA-EMX1

   Plasmid

   #244824

   Purpose

   Mammalian expression of SpyCas9 single guide RNA targeting EMX1

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpyCas9 single guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKMW124_dCas9

   Plasmid

   #244821

   Purpose

   Mammalian expression of catalytically inactive Cas9 (dCas9)

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   dCas9 (WT)
   

   Use

   CRISPR

   Tags

   3xFLAG-SV40 NLS and Nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   CAG, U6

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHS26_10xHis_MBP_TEV_VRER-Cas9

   Plasmid

   #244829

   Purpose

   Bacterial expression of VRER Cas9 for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   VRER Cas9
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   D1135V/G1218R/R1335E/T1337R

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHS16_10xHis_MBP_TEV_xPBA-Cas9

   Plasmid

   #244830

   Purpose

   Bacterial expression of xPBA Cas9 for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   xPBA Cas9
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHS71_10xHis_MBP_TEV_WT-Cas9_2xNLS

   Plasmid

   #244833

   Purpose

   Bacterial expression of SpyCas9 (WT) with 2xSV40 nuclear localization sequence for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpyCas9 (WT)
   

   Use

   CRISPR

   Tags

   10xHis-MBP and 2xSV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHS23_10xHis_MBP_TEV_SpG-Cas9

    Plasmid

    #244826

    Purpose

    Bacterial expression of SpG Cas9 for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    SpG Cas9
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHS1_10xHis_MBP_TEV_WT-Cas9

    Plasmid

    #244825

    Purpose

    Bacterial expression of SpyCas9 (WT) for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    SpyCas9 (WT)
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHS25_10xHis_MBP_TEV_EQR-Cas9

    Plasmid

    #244828

    Purpose

    Bacterial expression of EQR Cas9 for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    EQR Cas9
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    D1135E/R1335Q/T1337R

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHS9_10xHis_MBP_TEV_dCas9

    Plasmid

    #244831

    Purpose

    Bacterial expression of catalytically inactive Cas9 (dCas9) for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    dCas9 (WT)
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    D10A/H840A

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETSUMO2_Ideonella-bGSDM-WELQ

    Plasmid

    #222098

    Purpose

    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Ideonella species with a WELQut protease cleavage site for controlled activation.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

    Insert

    Ideonella-bGSDM-WELQ ORF
    

    Use

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21b-effector-ncRNA-rrt-His8

    Plasmid

    #194089

    Purpose

    Expresses Ec86 operon (effector, ncRNA and reverse transcriptase) in Escherichia coli

    Depositor

    Tingting Zou

    Article

    Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

    Insert

    Ec86 effector-ncRNA-rrt
    

    Use

    Tags

    His8

    Expression

    Mutation

    Promoter

    T7

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Interface-4 (b)

    Plasmid

    #204435

    Purpose

    Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

    Depositor

    Keir Neuman

    Article

    Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

    Insert

    hOpa1 isoform 1 (OPA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    I735A

    Promoter

    CMV

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Interface-1 (c)

    Plasmid

    #204430

    Purpose

    Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

    Depositor

    Keir Neuman

    Article

    Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

    Insert

    hOpa1 isoform 1 (OPA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q659A

    Promoter

    CMV

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Interface-1 (d)

    Plasmid

    #204431

    Purpose

    Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

    Depositor

    Keir Neuman

    Article

    Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

    Insert

    hOpa1 isoform 1 (OPA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    E679A

    Promoter

    CMV

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Interface-1 (e)

    Plasmid

    #204432

    Purpose

    Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

    Depositor

    Keir Neuman

    Article

    Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

    Insert

    hOpa1 isoform 1 (OPA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    H631A

    Promoter

    CMV

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Interface-5

    Plasmid

    #204436

    Purpose

    Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

    Depositor

    Keir Neuman

    Article

    Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

    Insert

    hOpa1 isoform 1 (OPA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    E444A

    Promoter

    CMV

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge