We narrowed to 7,541 results for: URE

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p15a-AsCas12f-blaZ

Plasmid

#171609

Purpose

AsCas12f1-based E. coli genome editing

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

AsCas12f1

Expression

Bacterial

Available Since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRT006.3 L1 dual-luciferase AI retrotransposition reporter

Plasmid

#213031

Purpose

Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration

Depositor

Donna Romero, Martin S. Taylor

Article

Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

Insert

Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker

Use

Luciferase

Tags

Firefly luciferase antisense intron (3' UTR)

Expression

Mammalian

Promoter

pTRE bidirectional tet-on

Available Since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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pJT260 Lenti pEF-dCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast

Plasmid

#187336

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pRSV-CaMKIV(1-313)

Plasmid

#45063

Purpose

Expression of constit active CaMKIV

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

Cam Kinase IV(1-313) (Camk4 Mouse)

Expression

Mammalian

Mutation

truncated at amino acid 313*

Promoter

RSV

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pET21b-RBD-JA1

Plasmid

#167126

Purpose

Expresses SARS-CoV-2 RBD in bacterial system

Depositor

David Margulies

Article

Ahmad et al bioRxiv. 2021 Jan 27. doi: 10.1101/2021.

Insert

SARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)

Expression

Bacterial

Mutation

residues (333 to 529 aa)

Promoter

T7

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSK23

Plasmid

#72851

Purpose

template for C-terminal eGFP tagging, Puromycin-resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

eGFP

Expression

Insect

Promoter

copia

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pUC18/promEco/Lysostaphin

Plasmid

#125765

Purpose

Production of staphylolytic protein lysostaphin from a constitutive promoter

Depositor

Benedykt Wladyka

Article

Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

Insert

lysostaphin

Expression

Bacterial

Mutation

sequence corresponding to the mature form protein

Promoter

pemIK-Sa1 promoter

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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HS-GFP-PACT

Plasmid

#105954

Purpose

heat shock inducible transgenesis, PACT is a centriol marker

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

PACT (AKAP9 Human)

Tags

GFP

Expression

Bacterial

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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nAChR beta2 CFP

Plasmid

#15106

Depositor

Henry Lester

Article

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

beta2-CFP-M (Chrnb2 Mouse)

Tags

CFP

Expression

Mammalian

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA3-mitoT

Plasmid

#174519

Purpose

Engineered intermembrane bridge to link the inner and outer mitochondrial membranes in mammalian cells

Depositor

Oleh Khalimonchuk

Article

Viana et al iScience. 2021 Jan 29;24(2):102119. doi:

Insert

MitoT synthetic tether

Tags

6xHis and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-AMPH1-BAR-mCherry

Plasmid

#85130

Purpose

BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry (AMPH Human)

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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NRXN-bio-His

Plasmid

#51810

Purpose

Expresses full-length Neurexin-1-alpha precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

NRXN (NRXN1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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Myc-Foxo1-WT 6KR

Plasmid

#17558

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 6KR (Foxo1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

K242R, K246R, K259R, K262R, K271R, K291R

Promoter

CMV

Available Since

Oct. 8, 2008

Availability

Academic Institutions and Nonprofits only
pGEX4T1-human beta-catenin full length

Plasmid

#132609

Purpose

expresses human beta-catenin full length in E. coli

Depositor

Wenqing Xu

Article

Xing et al Structure. 2008 Mar;16(3):478-87. doi: 1

Insert

beta-catenin

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-GFP-Sec61β

Plasmid

#186597

Purpose

Third generation lentiviral vector expressing GFP-Sec61β

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Sec61β (SEC61B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2+ centrin-mKate2

Plasmid

#105941

Purpose

in vitro transcription, zf centrin N-terminally tagged with mkate2

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Centrin

Tags

mKate2

Expression

Bacterial

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pMT-HuC:Dendra2

Plasmid

#80904

Purpose

Injection into Zebrafish larvae together with Tol2-Transposase to achieve pan-neuronal expression of the photoconvertible fluorescent protein Dendra2

Depositor

Periklis Pantazis

Article

Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

Insert

Dendra2 (elavl3 Synthetic)

Use

Zebrafish expression

Promoter

HuC

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry_LD 0_TEV

Plasmid

#58868

Purpose

MESA protease chain with mCherry ectodomain and no linker domain

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA protease chain with mCherry ectodomain and TEV protease

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt2b

Plasmid

#42275

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt2b (Wnt2b Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pCsy_complex

Plasmid

#89232

Purpose

Plasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage site

Depositor

Blake Wiedenheft

Article

Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

Inserts

Csy1
Csy2
Csy3
Csy4

Tags

6xHis-TEV protease

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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