162,257 results

1. VN-Tau (P301L)

   Plasmid

   #87634

   Purpose

   expresses hTau fused to the N-terminal part of Venus

   Depositor

   Tiago Outeiro

   Insert

   MAPT (MAPT Human)
   

   Use

   Tags

   N-terminal part of Venus

   Expression

   Mammalian

   Mutation

   Mutation P301L

   Promoter

   CMV

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKM493

   Plasmid

   #140191

   Purpose

   ORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Insert

   TEV-Flag-Gly4-eGFP
   

   Use

   Tags

   Flag + eGFP

   Expression

   Bacterial

   Mutation

   Promoter

   PGroEL

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pP35S (GB0030)

   Plasmid

   #68163

   Purpose

   Provides the CaMV 35S Promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   CaMV 35S promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. CL20_mEGFP_ETV6_RUNX1

   Plasmid

   #205818

   Purpose

   Express mEGFP-tagged fusion protein, ETV6_RUNX1 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   ETV6_RUNX1 (ETV6, RUNX1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENN.AAV.CAG.tdTomato.WPRE.SV40

   Plasmid

   #105554

   Purpose

   AAV expression of tdTomato from CAG promoter

   Depositor

   James M. Wilson

   Insert

   tdtomato
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TRE-OKSM

   Plasmid

   #204498

   Purpose

   Expresses Oct4, Klf4, Sox2 and c-Myc by tet-on promoter in mammalian cells

   Depositor

   Wataru Fujii

   Article

   Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

   Inserts

   OCT4 (POU5F1 Human)
   KLF4 (KLF4 Human)
   SOX2 (SOX2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAF0036 Ef1a-Si74-2a-EGFP

   Plasmid

   #193463

   Purpose

   Expression of Si74 recombinase from Ef1a promoter

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Si74
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. METTL3 shRNA 1 tet pLKO puro

   Plasmid

   #162985

   Purpose

   Tet-inducible shRNA targeting human METTL3 #1

   Depositor

   Mark Rubin

   Article

   Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-FOXA2

   Plasmid

   #153110

   Purpose

   Expression of Flag tagged human FOXA2 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXA2 (FOXA2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABE-puro LEF1

    Plasmid

    #27023

    Depositor

    Joan Massague

    Article

    Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

    Insert

    LEF1 (LEF1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF2958

    Plasmid

    #144434

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SP1 (SP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. yWL234 MCP-GFP-PABPC1*

    Plasmid

    #197054

    Purpose

    pUBC-NLS-HA-2xMCP-1xGFP-PABPC1*

    Depositor

    Robert Singer

    Article

    Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

    Insert

    pubc-nls-ha-MCP-GFP-PABPC1*
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ubc

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcdna3.1 hciap1

    Plasmid

    #8311

    Depositor

    Jon Ashwell

    Article

    Li et al Nature 2002 Mar 21;416(6878):345-7.

    Insert

    cIAP1 (BIRC2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYUB1062-GFP

    Plasmid

    #117695

    Purpose

    Express recombinant proteins with GFP-His fusion in Mycobacterium smegmatis

    Depositor

    Elizabeth Fullam

    Article

    Radhakrishnan et al RSC Adv. 2018 Sep 27;8(58):33087-33095.

    Type

    Empty backbone

    Use

    Tags

    GFP and His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti6.3-OGDH_wobble

    Plasmid

    #110938

    Purpose

    OGDH ORF containing silent wobble mutations insensitive to corresponding shRNAs

    Depositor

    William Hahn

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

    Insert

    OGDH wobble cDNA (OGDH Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    15 silent wobble mutations corresponding to three…

    Promoter

    SV40

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-Cry2olig

    Plasmid

    #208780

    Purpose

    Inducibly expresses light activatable Cry2 domain

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Cry2 mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-Epac2

    Plasmid

    #118314

    Purpose

    Expression of mouse Epac2

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2008 Dec;28(23):7109-25.

    Insert

    Epac2 (Rapgef4 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-PARP1

    Plasmid

    #215136

    Purpose

    Expresses mCherry-PARP1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-Opa1

    Plasmid

    #191963

    Purpose

    Expression of OPA1.

    Depositor

    Luca Scorrano

    Insert

    Opa1 (Opa1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDest-566

    Plasmid

    #11517

    Depositor

    Dominic Esposito

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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