165,971 results

1. pET28a-cMSP26

   Plasmid

   #199769

   Purpose

   Expresses split-intein fused MSP26 for circularization during expression (internal His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSP26
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   internal His-Tag

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-cMSPdH4-6

   Plasmid

   #199766

   Purpose

   Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSPdH46
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   internal His-Tag

   Available Since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSV Sport RXR alpha

   Plasmid

   #8882

   Depositor

   Bruce Spiegelman

   Article

   Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

   Insert

   RXR alpha (RXRA Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

   Plasmid

   #222994

   Purpose

   Mammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNA

   Depositor

   Jennifer Doudna

   Article

   Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

   Insert

   HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1
   

   Use

   CRISPR

   Tags

   Puro

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKO403_lacZ_Sp

   Plasmid

   #174726

   Purpose

   Bifidobacterium-E.coli shuttle vector for seamless gene cloning

   Depositor

   Tohru Suzuki

   Article

   Altaib et al Microbiol Resour Announc. 2022 Jan 20;11

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKSE401

   Plasmid

   #62202

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   maize codon optimized

   Promoter

   35S and AtU6-26p

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. IL6R PLAK1

   Plasmid

   #161518

   Purpose

   Expresses IL6 receptor (full length) in mammalian cells.

   Depositor

   Yoav Shaul

   Article

   Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

   Insert

   Interleukin 6 Receptor (IL6R Human)
   

   Use

   Lentiviral

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9

   Plasmid

   #184464

   Purpose

   Plasmid encoding PRDM9-Cas9 fusion under CMV promoter

   Depositor

   Jennifer Doudna

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

   Insert

   PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9 (PRDM9 Human)
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. KL063 - pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

   Plasmid

   #232929

   Purpose

   Targeting vector to introduce an OsTir1(F74G) expressing cassette at the mouse Rosa26 locus using Neomycin selection. AID2 degron system. Use with Addgene #64216 sgRNA.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   OsTir1(F74G)
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Mutation

   changed Phenylalanine 74 to Glycine

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-N LMP1

    Plasmid

    #37962

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    LMP1 (LMP-1 H. Herpesvirus 4 (EBV))
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    PKG

    Available Since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-OPA1

    Plasmid

    #26047

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    OPA1 (OPA1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO-CreERT2 stuffer v3

    Plasmid

    #158046

    Purpose

    lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pAAV-anti-PDL1(KN035) PAGER(TF)

    Plasmid

    #230003

    Purpose

    Expresses anti-PDL1 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    IL2SP-Aro6-KN035-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    V360A/R361A on KORD

    Promoter

    CMV

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti NbALFA-mNeonGreen puro

    Plasmid

    #193973

    Purpose

    lentivector coding for mNeonGreen fused to a nanobody targeting the ALFA tag, puromycin resistance

    Depositor

    Raphael Gaudin

    Insert

    NbALFA-mNeonGreen
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. 2X PRE TK luc

    Plasmid

    #11350

    Depositor

    Donald McDonnell

    Article

    Giangrande et al Mol Cell Biol. 2000 May . 20(9):3102-15.

    Insert

    PRE
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. mAIRN HO

    Plasmid

    #207411

    Purpose

    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    mAIRN
    

    Expression

    Bacterial and Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. mAIRN CD

    Plasmid

    #207412

    Purpose

    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    mAIRN
    

    Expression

    Bacterial and Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-myc-his-BACH1 WT

    Plasmid

    #17642

    Depositor

    Ronny Drapkin

    Article

    Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

    Insert

    BACH1 (BRIP1 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Available Since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. gCBEv3

    Plasmid

    #220618

    Purpose

    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv3) driven by EF1a promoter, guide RNAs compatible with SpG driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)

    Promoter

    hU6, EF1a

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSC101-Donor

    Plasmid

    #140630

    Purpose

    Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    LE, RE, KanR, temperature sensitive pSC101 origin of replication
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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