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We narrowed to 15,621 results for: nol

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Showing: 11601 - 11620 of 15621 results

  5. pET28-SpRY-HF1-NLS-6xHis (gbr2102)

    Plasmid
    #181744
    Purpose
    bacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    bacterial codon optimized SpRY-HF1
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS(SV40)-6xHis and MKIEE MBP tag
    Expression
    Bacterial
    Mutation
    SpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…
    Promoter
    T7
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    181744-plasmid-map-sequence-id-373171

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  7. pRaGE Pyl TAG GFP Y35TAG

    Plasmid
    #160041
    Purpose
    Encodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.
    Depositor
    Lital Alfonta
    Article
    Ozer et al Front Bioeng Biotechnol. 2021 Feb 26;9:5
    Inserts
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    deGFP
    Use
    Synthetic Biology
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed tyrosine 35 to TAG stop-codon (Site numbe…
    Promoter
    P70b promoter and T500 terminator, Vibrio natrieg…
    Available Since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    160041-plasmid-map-sequence-id-332393

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  9. pET24TEV-tSHIP1

    Plasmid
    #183770
    Purpose
    Expresses C-terminal truncated SHIP1 (tSHIP1)with C-terminal TEV and 6HIS tags from pET24 backbone
    Depositor
    William Kerr
    Article
    Sudan et al Front Immunol. 2022 May 4;13:830961. doi
    Insert
    SHIP1 (INPP5D Human)
    Tags
    6HIS and Tobacco Etch Virus (TEV) Cleavage site
    Expression
    Bacterial
    Mutation
    includes aa1 to aa883 only
    Promoter
    T7 lac
    Available Since
    June 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183770-plasmid-map-sequence-id-365832

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  11. Synapsin sHRPb-NLG in FSW lentiviral vector

    Plasmid
    #73148
    Purpose
    Small fragment of split HRP fused to the extracellular terminus of NLG1. This is a lentiviral plasmid, but can also be used for transfections. Syanpsin promoter.
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Insert
    sHRPb-NLG (Nlgn1 Synthetic, Mouse)
    Use
    Lentiviral
    Tags
    V5 tag
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73148-plasmid-map-sequence-id-134528

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Showing: 11601 - 11620 of 15621 results