174,916 results

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pCW-Tet-Cas9-BFP-PGK-Blast

Plasmid

#196720

Purpose

Tet-inducible expression of Cas9-T2A-TagBFP

Depositor

Bernhard Schmierer

Insert

Cas9-T2A-TagBFP

Use

CRISPR and Lentiviral

Tags

3xFLAG-SV40NLS and Nucleoplasmin NLS

Promoter

TRE, PGK

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-GFP-HiBiT

Plasmid

#198317

Purpose

Loads HiBiT-tagged GFP into PVCs using Pdp1 packaging domain; also contains Pnf

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-GFP-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-Cas9

Plasmid

#198275

Purpose

For loading SpCas9 into PVCs; also contains PVC regulatory genes

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-Cas9

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBR322-PVCpnf18-21

Plasmid

#198273

Purpose

Contains only regulatory genes; for generating empty PVCs

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf18-21

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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Sniper2L

Plasmid

#193856

Purpose

Expresses human codon-optimized Sniper2L and blasticidin resistance: EFS promoter-Sniper2L-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/

Insert

Sniper2L

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

E1007L

Promoter

EFS

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-RNaseHI-NES-EGFP

Plasmid

#196701

Purpose

Plasmid for stable expression of wild type bacterial RNase HI tagged with NES and EGFP that can be used to specifically degrade the DNA-RNA hybrids in the cytoplasm.

Depositor

Karlene Cimprich

Article

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

bacterial RNaseHI

Use

Lentiviral

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApoE3

Plasmid

#197147

Purpose

Expresses ApoE3 in Mammalian cell

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

ApoE3 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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plentiCMV-sct-pp65-b2m-HLA-A0201

Plasmid

#196511

Purpose

Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

single chain of a pp65 peptide, b2m and HLA A0201

Use

Lentiviral

Tags

NA

Expression

Mammalian

Mutation

Y84C

Promoter

CMV

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE3g-mNeon-WPRE-pA

Plasmid

#192064

Purpose

mNeonGreen expression with TRE3g promoter

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

mNeonGreen

Use

AAV

Expression

Mammalian

Promoter

TRE3g

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-bARGSer-AxeTxe

Plasmid

#192473

Purpose

Second-generation bacterial acoustic reporter gene derived from Serratia

Depositor

Mikhail Shapiro

Article

Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Inserts

bARGSer
AxeTxe

Expression

Bacterial

Mutation

the gene Ser39006_001280 was deleted from the clu…

Promoter

Native promoter from Enterococcus faecium and pBAD

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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CapChR2_pmScarlet-N1

Plasmid

#188032

Purpose

Expresses CapChR2-mScarlet in mammalian cells

Depositor

Peter Hegemann

Article

Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

Insert

CapChR2-mScarlet

Tags

mScarlet

Expression

Mammalian

Mutation

S43D, L112C, T139C and N238E mutations in CoChR

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAXY0001

Plasmid

#179834

Purpose

Binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

Depositor

Xiaohan Yang

Article

Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

Insert

eYGFPuv

Use

Binary vector for plant transformation

Expression

Plant

Promoter

35S

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA C12 ecDHFR DD YFP HA

Plasmid

#192815

Purpose

Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.

Depositor

John Hulleman

Article

Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021

Insert

E. coli dihydrofolate reductase

Tags

enhanced yellow fluorescent protein and hemagglut…

Expression

Mammalian

Mutation

W74R/T113S/E120D/Q146L

Promoter

CMV

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-NEMOc

Plasmid

#189933

Purpose

Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

Depositor

Yubin Zhou

Article

Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

Insert

NEMOc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ782 - HygroR

Plasmid

#190933

Purpose

Hygromycin resistance co-injection marker

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Hygromycin resistance gene

Expression

Worm

Promoter

rps-0

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-p21

Plasmid

#171122

Purpose

Doxycycline inducible expression of p21

Depositor

Jason Sheltzer

Article

Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

Insert

p21 (CDKN1A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3GS

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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p8.91

Plasmid

#187441

Purpose

Lentiviral packaging plasmid expressing gag and pol.

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Inserts

Use

Lentiviral

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pSBbi_RP_MicroID2-HA

Plasmid

#188521

Purpose

Encodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cells

Depositor

James Londino

Article

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

MicroID2

Use

Transposon mediated gene integration

Tags

HA

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ10875

Plasmid

#183963

Purpose

CBA-hyperdCas12a-HA-KRAB-mCherry

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Insert

HyperdCas12a

Use

CRISPR

Tags

HA, KRAB, and mCherry

Expression

Mammalian

Mutation

D832A, D156R, E292R, D235R, D350R

Promoter

CBA

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-EML4-ALK variant 3a

Plasmid

#183829

Purpose

Express EML4-ALK fusion variant 3a (E6a;A20) in mammalian cells

Depositor

Aaron Hata

Article

Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

Inserts

ALK Receptor Tyrosine Kinase (ALK Human)
EMAP Like 4 (EML4 Human)

Use

Lentiviral

Tags

The fusion protein of EML4 exon 1-6a and ALK exon…

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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