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19,771 results

Showing: 1741 - 1770 of 19771 results

  11. pHN924

    Plasmid
    #178885
    Purpose
    Expression of GST-His8-HRV3C
    Depositor
    Kazuhiro Abe
    Insert
    human rhinovirus 3C protease
    Tags
    GST, His8
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    178885-plasmid-map-sequence-id-354971

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  19. pdCas9-DNMT3A-EGFP

    Plasmid
    #71666
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71666-plasmid-map-sequence-id-129527

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  21. pETcon(-)

    Plasmid
    #41522
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Tags
    3x(G4S) Linker, G3S Linker, HA, Myc Tag, and Stuf…
    Expression
    Yeast
    Promoter
    GAL1
    Available Since
    April 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    41522-plasmid-map-sequence-id-59598

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  22. pAAV2/7

    Plasmid
    #112863
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap7
    Use
    AAV
    Promoter
    Truncated P5
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    112863-plasmid-map-sequence-id-364707

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  26. lentiGuide-Hygro-iRFP670

    Plasmid
    #99377
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A iRFP670 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Article
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-iRFP670
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available Since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99377-plasmid-map-sequence-id-191028

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  29. lenti-sgRNA hygro

    Plasmid
    #104991
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104991-plasmid-map-sequence-id-335144

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Showing: 1741 - 1770 of 19771 results