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pAGM22082_cRed

Plasmid

#117225

Purpose

pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

Depositor

Sylvestre Marillonnet

Publication

Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10

Insert

Canthaxanthin Operon

Tags

34 amino acid N-terminal linker, Hexahistidine Ta…

Expression

Bacterial

Mutation

removed Type IIS restriction sites within the bac…

Promoter

T7 RNA Polymerase

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pTubulin-mTagBFP2

Plasmid

#184931

Purpose

Tubulin labeling with blue fluorescent protein mTagBFP2

Depositor

Kiryl Piatkevich

Publication

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TUBA1B-mTagBFP2

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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PEX-LV

Plasmid

#12120

Depositor

Inder Verma

Publication

Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Matrix Metalloproteinase 2 (Mmp2 Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

noncatalytic fragment of MMP2 comprised of the fi…

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

Plasmid

#166693

Purpose

Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.

Depositor

Elvir Becirovic

Publication

Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

dCas9-VPR
3x Cnga1 promoter-targeting sgRNAs

Use

CRISPR

Expression

Mammalian

Promoter

TRE and U6

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-LATS2

Plasmid

#166474

Purpose

Expresses fusion of mEGFP and LATS2

Depositor

Shuguo Sun

Publication

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mEGFP, LATS2 (LATS2 Human)

Expression

Mammalian

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_Ado1.0med

Plasmid

#153289

Purpose

Express the medium-affinity adenosine sensor GRAB_Ado1.0med in astrocytes

Depositor

Yulong Li

Publication

Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Adenosine sensor GRAB_Ado1.0med (medium affinity)

Use

AAV

Promoter

GfaABC1D

Available since

Aug. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pF CAG BFP2 IRES neo

Plasmid

#108175

Purpose

Expresses a blue fluorescent protein (BFP2) in mammalian cells along with conferring resistance to G418

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

Blue fluorescent protein (BFP2)

Use

Lentiviral; Fluorescent protein

Expression

Mammalian

Promoter

CAG

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLCKO_LacZ_sgRNA

Plasmid

#74179

Purpose

lentiviral vector expressing sgRNA targeting LacZ

Depositor

Jason Moffat

Publication

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

LacZ sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 Promoter

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FUW-Mbd3

Plasmid

#52356

Purpose

Lentiviral expression vector for constitutive expression of Mbd3

Depositor

Jacob Hanna

Publication

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Ubiquitin

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET24b-BR1.4

Plasmid

#199436

Purpose

expresses BAmRed1.4 in bacterial cells

Depositor

Andrew Woolley

Publication

Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

Insert

BAMRed1.4

Tags

his tag

Expression

Bacterial

Promoter

T7

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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6x-His_A1aY1_GFP

Plasmid

#158753

Purpose

Bacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification

Depositor

Minhajuddin Sirajuddin

Publication

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

6xHistidine_A1aY1_GFP

Tags

6x-Histidine tag, GFP, T7 leader sequence, and Th…

Expression

Bacterial

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSKC11 - UTRQ

Plasmid

#110490

Purpose

Monitor the expression of Luciferase driven by the NRAS 5'-UTR.

Depositor

Shankar Balasubramanian

Publication

Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NRAS 5'-UTR (bp 1-254) (NRAS Human)

Use

Luciferase

Tags

Firefly luciferase

Promoter

T7

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP_sfCherry2(11)_zyxin

Plasmid

#83034

Purpose

Expresses sfCherry2(11) tagged zyxin (human) in mammalian cells

Depositor

Bo Huang

Publication

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sfCherry2(11)_zyxin

Expression

Mammalian

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pQCXIH-HA NEDD4L

Plasmid

#27028

Depositor

Joan Massague

Publication

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4L (NEDD4L Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pEMS1277

Plasmid

#29140

Purpose

Plasmid described in Yang et al., Genomics, 2009 (PMID: 18950699).

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CAG promoter

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pAGM1311

Plasmid

#47983

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pME minprom KalTa4

Plasmid

#195964

Purpose

Gateway pME Gal4 variant

Depositor

Alexa Burger, Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mouse beta-globin minimal promoter KalTA4 (Gal4)

Use

Other

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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458 p3E-2A-EGFP-CAAXpA

Plasmid

#195968

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-EGFP-CAAX (membrane)

Use

Other

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX301-TagRFP

Plasmid

#162035

Purpose

Tagged vector control

Depositor

David Bryant

Publication

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Type

Empty backbone

Use

Lentiviral

Tags

RFP

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDNA5/avitag-apex2-v5-fkbp8

Plasmid

#154460

Purpose

expresses avitag-apex2-v5-fkbp8/biochemical assay

Depositor

Hyun-Woo Rhee

Publication

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

avitag-apex2-v5-fkbp8 (FKBP8 Human)

Expression

Mammalian

Promoter

CMV-F, T7 promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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