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pMyc1ElbLuc,

Plasmid

#53243

Purpose

reporter plasmid containing 1 myc cDNA binding sites

Depositor

Roger Davis

Article

Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

Insert

pMyc1ElbLuc (MYC Human)

Mutation

To prepare plasmids with one, two, three, and fou…

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-RNF17L

Plasmid

#35413

Depositor

Jeremy Wang

Article

Pan et al Development. 2005 Sep;132(18):4029-39. E

Insert

Rnf17l (Rnf17 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-TAT-2-130-FLAG

Plasmid

#35984

Depositor

Susana Valente

Article

Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

Insert

transactivating protein (tat )

Tags

Flag

Expression

Mammalian

Mutation

tat from HIV2 130 amino acids

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pEF/myc/ER FLIPglu-30uDelta13V

Plasmid

#18021

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu D154A Delta13

Tags

ECFP and Venus

Expression

Mammalian

Mutation

mglB-D154A

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pNLS-2xBFP(4C)

Plasmid

#176151

Purpose

Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.

Depositor

Siegfried Musser

Article

Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

Insert

NLS-2xBFP(4C)

Tags

6xHis tag and SV40 nuclear localization sequence …

Expression

Bacterial

Mutation

Compared with EGFP, the two BFP domains have the …

Promoter

Ptrc

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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AF1521-c002

Plasmid

#173109

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))

Tags

mCerulean

Expression

Bacterial

Mutation

KH-M1-L192

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2442 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159858

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-BHLHE40(S243A/E248A)

Plasmid

#110155

Purpose

Expression of human BHLHE40 (S243A/E248A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

Insert

BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)

Tags

HA

Expression

Mammalian

Mutation

S243A/E248A

Promoter

CMV

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pZCS36 (Phsp16.41::Cas9(dpiRNA)::tbb-2 ‘3UTR)

Plasmid

#193048

Purpose

Heatshock expressed Cas9

Depositor

Patrick Phillips

Article

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

hsp16.41p::Cas9(dpiRNA)::tbb-2 ‘3UTR

Use

CRISPR

Expression

Worm

Promoter

hsp16.41p

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pZCS38 (Prsp-27::NeoR::unc-54 3’ UTR)

Plasmid

#193049

Purpose

Encodes G-418 resistance

Depositor

Patrick Phillips

Article

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

prsp-27::NeoR::unc-54 3’ UTR

Expression

Worm

Promoter

prsp-27

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-HAND2

Plasmid

#170690

Purpose

doxycycline-inducible overexpression of HAND2

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

HAND2 (HAND2 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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EC2_3_dCas9_Mxi1_sgRNA

Plasmid

#163708

Purpose

Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
Mxi1+SV40 NLS

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pmEos2-N1 GBP (GBP-mEos2)

Plasmid

#162876

Purpose

Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

GFP Binding Protein tagged with mEos2

Tags

mEos2

Expression

Mammalian

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDRF'- AmTryoshka1;3-LS-F138I-T78H

Plasmid

#100963

Purpose

Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

AmTryoshka1;3-LS-F138I-T78H (AMT1;3 Mustard Weed)

Expression

Yeast

Mutation

F138I, T78H

Promoter

PMA

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF8-C-terminus

Plasmid

#64673

Purpose

expresses only C terminus of RNF8, amino acids 402-485

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Ring finger protein 8 (RNF8 Human)

Tags

GFP

Expression

Mammalian

Mutation

containing only C-terminus amino acids 402 to 485

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
Flag Snail 4SE

Plasmid

#16223

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

Flag

Expression

Mammalian

Mutation

4SE: S108E, S112E, S116E, S120E

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
AKAP1-EGFP-uTEV1

Plasmid

#171005

Purpose

HiLITR protease with AKAP1 N-terminal tmd targeting information (Mitochondria)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

AKAP1(tmd)-EGFP-uTEV1 (AKAP1 Synthetic)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

pTRE-tight (TetOn)

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAdCMV/V5-DEST-S727A-STAT3-3xFlag

Plasmid

#99262

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3 (S727A)

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - S727A (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Mutation

S727A

Promoter

CMV

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pSLIKneo 3XFLAG-wtGATA6-3XAU1_RNAi resistant

Plasmid

#72616

Purpose

Expresses an inducible and RNAi resistant, N-terminally 3X-FLAG tagged, C-terminally 3X-AU1 tagged wtGATA6

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA Binding Protein 6 (GATA6 Human)

Use

Lentiviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Promoter

TRE tight

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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Puro-TTL-wt-TTF-1

Plasmid

#67657

Purpose

To create a dox-on inducible expression of human wt-TTF-1 cDNA in cells

Depositor

David Mu

Article

Rice et al J Biol Chem. 2013 Jun 7;288(23):16348-60

Insert

Thyroid Transcription Factor 1 (NKX2-1 Human)

Use

Retroviral

Tags

N/A

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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