159,617 results

1. mPA-GFP-Calnexin-N-14

   Plasmid

   #57122

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Insert

   Calnexin (CANX Human)
   

   Use

   Tags

   mPA-GFP

   Expression

   Mammalian

   Mutation

   I474N in Calnexin

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

2. U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1

   Plasmid

   #92120

   Purpose

   Expression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   MS2-P65-HSF1 (HSF1 Human, Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. IL6R PLAK1

   Plasmid

   #161518

   Purpose

   Expresses IL6 receptor (full length) in mammalian cells.

   Depositor

   Yoav Shaul

   Article

   Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

   Insert

   Interleukin 6 Receptor (IL6R Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LeGO-hEµMAR-G2

   Plasmid

   #52633

   Purpose

   3rd generation lentiviral vector for enhanced eGFP expression/marking in primary B cells and B-cell lines

   Depositor

   Boris Fehse

   Article

   Mock et al Hum Gene Ther Methods. 2012 Dec;23(6):40

   Insert

   hEµMAR-SFFV; eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pU6-sgRNA Ef1alpha Puro-T2A-GFP

   Plasmid

   #111596

   Purpose

   sgRNA Ef1alpha Puro-T2A-GFP

   Depositor

   Jonathan Weissman

   Article

   Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

   Insert

   mU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMC-1-StUbi-6

   Plasmid

   #197710

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   StUbi promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJAT32

   Plasmid

   #204297

   Purpose

   pJAT plasmid with multiple cloning site for introducing custom payload. Two U6 promoters.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHDR-FOXL2-T2A-tdTomato-PuroTK

   Plasmid

   #192892

   Purpose

   HDR donor for inserting FOXL2-T2A-tdTomato reporter

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   FOXL2-tdTomato (FOXL2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   T2A-tdTomato fluorescent reporter

   Promoter

   PGK

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HA-Clover

   Plasmid

   #163366

   Purpose

   Backbone for generating N-terminal or C-terminal clover constructs

   Depositor

   Violaine Sée

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHSB04X

    Plasmid

    #117258

    Purpose

    Genome editing for Lactobacillus brevis ATCC367

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    Cas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TagRFP-T-EEA1

    Plasmid

    #42635

    Depositor

    Silvia Corvera

    Article

    Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Tags

    TagRFP-T

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSC101_TIMER

    Plasmid

    #103057

    Purpose

    low copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

    Depositor

    Dirk Bumann

    Article

    Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

    Insert

    TIMER-bac
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutations: S4T and S179T

    Promoter

    constitutive ybaJp promoter

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Ga13_2-mTq2

    Plasmid

    #112933

    Purpose

    Human Galpha13 tagged with mTurquoise, inserted at residue 128

    Depositor

    Dorus Gadella

    Article

    Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

    Insert

    Galpha13
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mPA-GFP-Farnesyl-5

    Plasmid

    #57133

    Purpose

    Localization: Membrane, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pCMV-STAT6-IRES-Neo

    Plasmid

    #35482

    Depositor

    Axel Nohturfft

    Insert

    STAT6 (STAT6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONRPEX18Tp-SceI-pheS

    Plasmid

    #111608

    Purpose

    Gateway compatible allelic exchange vector for Burkholderia sp.

    Depositor

    Tim Tolker-Nielsen

    Article

    Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

    Type

    Empty backbone

    Use

    Allelic exchange in burkholderia sp.

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-EML4-ALK variant 1

    Plasmid

    #183828

    Purpose

    Express EML4-ALK fusion variant 1 (E13;A20) in mammalian cells

    Depositor

    Aaron Hata

    Article

    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-13 and ALK exon…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MAC_N_ETV4

    Plasmid

    #167753

    Purpose

    Expresses ETV4 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    ETV4 (ETV4 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLQ7556 pHR (hU6-crCD46-EFS-PuroR-WPRE)

    Plasmid

    #214880

    Purpose

    Lentiviral vector encoding RfxCas13d targeting CD46 guide

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crCD46-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Neurofascin-186-HA

    Plasmid

    #31061

    Depositor

    Vann Bennett

    Article

    Zhang et al J Biol Chem. 1998 Nov 13. 273(46):30785-

    Insert

    Neurofascin (Nfasc Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only