162,408 results

1. pENTR/D_creERt2

   Plasmid

   #27321

   Depositor

   Leonard Zon

   Article

   Mosimann et al Development. 2011 Jan . 138(1):169-77.

   Insert

   creERt2
   

   Use

   Multisite gateway middle entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti6.3-OGDH_wobble

   Plasmid

   #110938

   Purpose

   OGDH ORF containing silent wobble mutations insensitive to corresponding shRNAs

   Depositor

   William Hahn

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

   Insert

   OGDH wobble cDNA (OGDH Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   15 silent wobble mutations corresponding to three…

   Promoter

   SV40

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. mTurquoise2 WC visual barcode

   Plasmid

   #158669

   Purpose

   tagging clonal populations with whole cell mTurquoise2 fluorescent protein

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mTurquoise2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. CRISPR_HLA_class_I_2

   Plasmid

   #164987

   Purpose

   Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   gRNA against HLA class I
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR_Gal4UAS_4D5-High-CAR-mCherry_PGK_4D5-Low-SynNotch_Gal4VP64_RHL133

   Plasmid

   #164822

   Purpose

   Lentiviral vector for Gal4 inducible AntiHER2 4D5-High CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch

   Depositor

   Wendell Lim

   Article

   Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

   Inserts

   AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
   AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   miniCMV and pGK

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSIM24-GUS

   Plasmid

   #67882

   Purpose

   Plant Gene Expression Vector pSIM24 containing the GUS (Beta-glucuronidase) reporter gene

   Depositor

   Indu Maiti

   Article

   Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

   Insert

   uidA
   

   Use

   Tags

   Expression

   Plant

   Mutation

   T120A

   Promoter

   M24

   Available since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-PhoCl1-6His

   Plasmid

   #164033

   Purpose

   Expressed PhoCl1 in a pET expression vector

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   PhoCl1
   

   Use

   Tags

   6xHis Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF2958

   Plasmid

   #144434

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SP1 (SP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLTR-Rabies PV

   Plasmid

   #190897

   Purpose

   Pseudotyping of lentiviral vectors

   Depositor

   Jakob Reiser

   Article

   Federici et al Genet Vaccines Ther. 2009 Jan 13;7:1. do

   Insert

   Lyssavirus rabies glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   HIV-1 LTR

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. TetR in pENTR1A (559-1)

    Plasmid

    #22265

    Depositor

    Eric Campeau

    Insert

    Tetracycline repressor (tetR )
    

    Use

    Tags

    Expression

    Insect, Mammalian, Worm Exp…

    Mutation

    Promoter

    Available since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. L4440_BioBrick-hsf-1-sgRNA-B

    Plasmid

    #177787

    Purpose

    Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    hsf-1 (hsf-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-6His-MBP-MpAgo

    Plasmid

    #79249

    Purpose

    This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression

    Depositor

    Jennifer Doudna

    Article

    Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p

    Insert

    Marinitoga piezophila Argonaute
    

    Use

    CRISPR

    Tags

    6XHis-tag + MBP + PreScission Cleavage Site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA6 asyn E46K

    Plasmid

    #105730

    Purpose

    Mammalian expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

    Insert

    alpha-synuclein E46K (SNCA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E46K

    Promoter

    CMV

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pnCS_SEPT7_SEPT9_i1-TEV-Strep

    Plasmid

    #174500

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i1

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    

    Use

    Tags

    TEV-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. single polypeptide FPX biosensor for calcium ion

    Plasmid

    #60887

    Purpose

    FPX biosensor

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for calcium ion
    

    Use

    Tags

    HindIII site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGGDestTol2LC-5sgRNA

    Plasmid

    #64243

    Purpose

    destination vector for five U6x:sgRNA cassettes

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQP-iRIS

    Plasmid

    #102584

    Purpose

    Regulation of protein localization (near-infrared-blue-light-inducible shuttle)

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

    Insert

    BphP1-mVenus-CAAX-IRES2-NES-mCherry-QPAS1-AsLOV2cNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. mt POLRMT

    Plasmid

    #205059

    Purpose

    POLRMT protein expression

    Depositor

    Smita Patel

    Article

    Ramachandran et al Nucleic Acids Res. 2017 Jan 25;45(2):861

    Insert

    POLRMT (POLRMT Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized

    Promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-TetOne-Puro-mitoEcSTH-FLAG

    Plasmid

    #218629

    Purpose

    This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    mitoEcSTH
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG and MTS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. lenti-CDDR

    Plasmid

    #149355

    Purpose

    CRISPR/Cas9-based dual-fluorescent DSB repair reporter

    Depositor

    Tarek Abbas

    Article

    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

    Inserts

    CDDR Reporter
    Blasticidin resistance
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hPGK

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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