Skip to main content
Addgene
Showing: 201 - 240 of 348 results

  1. miReporter-PGK

    Plasmid
    #82477
    Purpose
    microRNA reporter relying on a bidirectional PGK promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.
    Depositor
    Pierre Neveu
    Article
    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi
    Insert
    H2B-mCherry and H2B-Citrine. HygroR
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    82477-plasmid-map-sequence-id-163461

    Enlarge

  2. miReporter-CAG

    Plasmid
    #82478
    Purpose
    microRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.
    Depositor
    Pierre Neveu
    Article
    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi
    Insert
    H2B-mCherry and H2B-Citrine. HygroR
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    82478-plasmid-map-sequence-id-174379

    Enlarge

  3. pNOC-RLUC

    Plasmid
    #98142
    Purpose
    Gateway compatible construct for N' terminal fusion or promoter driven renilla luciferase reporter, hygromycin resistance cassette
    Depositor
    Eva Farre
    Article
    Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10
    Type
    Empty backbone
    Use
    Algae, nannochloropsis
    Tags
    HA
    Expression
    Mutation
    Promoter
    Available since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    98142-plasmid-map-sequence-id-210244

    Enlarge

  4. pNOC-NLUC

    Plasmid
    #98141
    Purpose
    Gateway compatible construct for N' terminal fusion or promoter driven NanoLuciferase reporter, hygromycin resistance cassette
    Depositor
    Eva Farre
    Article
    Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10
    Type
    Empty backbone
    Use
    Algae, nannochloropsis
    Tags
    HA
    Expression
    Mutation
    Promoter
    Available since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    98141-plasmid-map-sequence-id-210253

    Enlarge

  5. pNOC-FLUC

    Plasmid
    #98140
    Purpose
    Gateway compatible construct for N' terminal fusion or promoter driven firefly luciferase reporter, hygromycin resistance cassette
    Depositor
    Eva Farre
    Article
    Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10
    Type
    Empty backbone
    Use
    Algae, nannochloropsis
    Tags
    HA
    Expression
    Mutation
    Promoter
    Available since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    98140-plasmid-map-sequence-id-210257

    Enlarge

  6. pAC574

    Plasmid
    #127975
    Purpose
    AMA1 plasmid with hygB selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    hygB (hygromycin resistance marker)
    Use
    Fungal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans trpC promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    127975-plasmid-map-sequence-id-254311

    Enlarge

  10. SOX17-tdTomato-EhB

    Plasmid
    #210467
    Purpose
    Donor plasmid for knock-in tdTomato into the human SOX17 locus
    Depositor
    Kai Wang
    Insert
    SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived hygromycin-NLS-mTagBFP2 selection cassette (SOX17 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    210467-plasmid-map-sequence-id-419023

    Enlarge

  12. Pvalb-2A-Dre Flp-in replacement vector

    Plasmid
    #61571
    Purpose
    Recombinase-mediated cassette exchange in ES cells to insert the Dre recombinase gene at the mouse Pvalb gene stop codon
    Depositor
    Hongkui Zeng
    Article
    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10
    Insert
    Pvalb-2A-Dre (Pvalb Mouse, D6 Bacteriophage)
    Use
    Recombinase-mediated cassette exchange using flp …
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    61571-plasmid-map-sequence-id-100354

    Enlarge

  13. L1-AA027

    Plasmid
    #185752
    Purpose
    Level 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a).
    Depositor
    Peter Szoevenyi
    Article
    Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph
    Insert
    HygR2
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    185752-plasmid-map-sequence-id-367857

    Enlarge

  30. pNOC-CRISPR

    Plasmid
    #100008
    Purpose
    Expresses Cas9-Nlux and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into genome
    Depositor
    Eva Farre
    Article
    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968
    Type
    Empty backbone
    Use
    Algae, nannochloropsis expression
    Tags
    Cas9-Nlux
    Expression
    Mutation
    Promoter
    Ribi promoter
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    100008-plasmid-map-sequence-id-201983

    Enlarge

  31. pSF522

    Plasmid
    #222588
    Purpose
    BB3 ZWF1 tet-on overexpression cassette with homologous flanks for integration at pyrG locus
    Depositor
    Matthias Steiger
    Article
    Matthias et al PLOS One
    Insert
    ZWF1
    Use
    A. niger
    Tags
    Expression
    Bacterial
    Mutation
    Internal BsaI and BpiI sites removed from insert
    Promoter
    tet-on
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    222588-plasmid-map-sequence-id-450454

    Enlarge

  33. pENTR-DMD-Donor

    Plasmid
    #60605
    Purpose
    Knock-in donor vector to insert Exon 44 in front of Exon 45 of human DYSTROPHIN (DMD)gene, include EF1a-hygromycin resistance cassette flanked by loxP sequences.
    Depositor
    Akitsu Hotta
    Article
    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22
    Insert
    Hygromycin resistant gene
    Use
    Knock-in donor vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    60605-plasmid-map-sequence-id-97115

    Enlarge

  34. pNOC-CRISPR-GFP

    Plasmid
    #100009
    Purpose
    Expresses Cas9-GFP and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into geneome
    Depositor
    Eva Farre
    Article
    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968
    Type
    Empty backbone
    Use
    Algae, nannochloropsis expression
    Tags
    Cas9-GFP
    Expression
    Mutation
    Promoter
    Ribi promoter
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    100009-plasmid-map-sequence-id-202006

    Enlarge

Showing: 201 - 240 of 348 results