-
Plasmid#60985PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound EBFP2.DepositorInsertEBFP2
UseZebrafishTagsmembrane tagExpressionMutationPromoterBactin2Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTG03-AmpFRT
Plasmid#128272PurposeTIP-LITer1.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP---CMV-TetOx2-GFPDepositorInsertsTetR-LOV-TIP
EGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTG04-AmpFRT
Plasmid#128273PurposeDeg-LITer1.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-RRRG---CMV-TetOx2-GFPDepositorInsertsTetR-LOV-RRRG
EGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mO
Plasmid#60989PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound mKODepositorInsertmKO
UseZebrafishTagsmembrane tagExpressionMutationPromoterBactin2Available sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mY
Plasmid#60988PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound citrineDepositorInsertcitrine
UseZebrafishTagsmembrane tagExpressionMutationPromoterBactin2Available sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT3-RhoAC
Plasmid#100032PurposeThis plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with adenylyl cyclase activity.DepositorInsertRhoAC
UseTags1D4 (TETSQVAPA)ExpressionMammalianMutationE497K/C566DPromoterAdenovirus major late promoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-hO
Plasmid#60996PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear mKODepositorInsertmKO
UseZebrafishTagshistone 2B tagExpressionMutationPromoterBactin2Available sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mC
Plasmid#60986PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound ceruleanDepositorInsertCerulean
UseZebrafishTagsmembrane tagExpressionMutationPromoterBactin2Available sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-R
Plasmid#60983PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic dTomatoDepositorInsertdTomato
UseZebrafishTagsExpressionMutationPromoterBactin2Available sinceFeb. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-Y
Plasmid#60981PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic citrineDepositorInsertcitrine
UseZebrafishTagsExpressionMutationPromoterBactin2Available sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-fR
Plasmid#60984PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic mKate2.DepositorInsertmKate2
UseZebrafishTagsExpressionMutationPromoterBactin2Available sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-G
Plasmid#60980PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic EGFPDepositorInsertEGFP
UseZebrafishTagsExpressionMutationPromoterBactin2Available sinceJan. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT-C34-H
Plasmid#223966PurposeAnti-MT-C34 heavy chain expression plasmid; to be used with the light chain plasmid, pMT-C34-L (Plasmid 223967), to make the antibody.DepositorInsertVariable domain of mouse anti-MT-C34 IgG1 heavy chain jointed to constant region of human IgG1 heavy chain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT-C34-L
Plasmid#223967PurposeAnti-MT-C34 light chain expression plasmid; to be used with the heavy chain plasmid, pMT-C34-L (Plasmid 223966), to make the antibody.DepositorInsertVariable domain of mouse anti-MT-C34 IgG1 light chain jointed to constant region of human IgG1 light chain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
An HA CAAX pMT2
Plasmid#206116Purposeexpression of Cav2.1 N-terminus (amino acids 1-100) with a C-terminal CAAX (last 10aa of H.Ras) motif and a HA tagDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationPromoterAd MLP/TPL/SV40Available sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
An CAAX pMT2
Plasmid#206118Purposeexpression of Cav2.1 N-terminus (amino acids 1-100) with C-terminal CAAX (last 10 aa of H.Ras) motifDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationPromoterAd MLP/TPL/SV40Available sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT335-mScarletI
Plasmid#174506PurposeHigh-copy number plasmid (pMT335) harboring the mScarletI gene for its cytoplasmic expression, inducible by vanillate (sequence of mScarletI optimized)DepositorInsertmScarletI
UseTagsmScarletIExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMtb MCM
Plasmid#138296PurposeExpression of Mtb MCM small and large subunitDepositorInsertsmutA
mutB
UseTagsHis tag and TEV cleavage siteExpressionBacterialMutationinserted TEV cleavage site between His-tag and m…PromoterT7Available sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPMtb (GB0080)
Plasmid#68167PurposeProvides the S. Lycopersicum metallothionein II-like protein (MTB) gene promoter as a level 0 GoldenBraid partDepositorInsertSlMtb promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-C
Plasmid#60979PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic ceruleanDepositorInsertCerulean
UseZebrafishTagsExpressionMutationPromoterBactin2Available sinceFeb. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-O
Plasmid#60982PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic mKODepositorInsertmKO
UseZebrafishTagsExpressionMutationPromoterBactin2Available sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmTgfa UTR
Plasmid#31256DepositorInsertTgfa (Tgfa Mouse)
UseCdna for in situ hybridizationTagsExpressionMutationExon 6 of Tgfa with 3'UTRPromoterAvailable sinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMTG06-Amp-FRT
Plasmid#128275PurposeDeg-LITer2.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-RRRG-P2A-GFPDepositorInsertsTetR-LOV-RRRG
EGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-sgAAVS1-HepmTurquoise2
Plasmid#192828PurposeExpresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mTurquoise2 from hepatocyte-specific promoterDepositorInsertmTurquoise2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 for sgRNA and hepatocyte-specific promoter (HS…Available sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmtb-t7-alpha-bungarotoxin
Plasmid#69542PurposeFor in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.DepositorInsertalpha-bungarotoxin
UseTol2, beta-actin promoter, sp6,t7 for zebrafishTagsExpressionMutationPromoterbeta-actin, sp6, t7Available sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-URH49 WT
Plasmid#110127PurposeExpresses Flag-tagged human URH49 (DDX39A)DepositorInsertURH49 (DDX39A Human)
UseTagsFlagExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceApril 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTH PKC alpha
Plasmid#8409DepositorInsertMouse Protein Kinase C alpha (Prkca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
Human SERCA3 (pMT2)
Plasmid#75189PurposeMammalian expression of ATP2A3DepositorInsertATP2A3 (ATP2A3 Human)
UseTagsExpressionMammalianMutationPromoteradenovirus major late promoterAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmTurqoise KIF1A shRNA
Plasmid#102849PurposeGenerates shRNA specific to Rat KIF1A along with cell fill pmTurqoiseDepositorInsertKIF1A-shRNA (KIF1A Rat)
UseRNAiTagsExpressionMammalianMutationPromoterH1-RNAAvailable sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT-Hrp48-ADARcd-V5
Plasmid#81172Purposeidentification of cell-specific targets of RNA binding proteinsDepositorInsertHrp48-ADARcd fusion (Hrb27C Fly)
UseTagsExpressionInsectMutationPromoterDrosophila metallothionein promoterAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT2 Beta1b GFP
Plasmid#89893PurposeMammalian expression of Beta1b GFPDepositorInsertCacnb1 (Cacnb1 Rat)
UseTagsGFPExpressionMammalianMutationGFP sequence inserted just before the Stop codonPromoteradenovirus major late promoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT2 Beta1b mCherry
Plasmid#89892PurposeMammalian expression of Beta1b mCherryDepositorInsertCacnb1 (Cacnb1 Rat)
UseTagsmCherryExpressionMammalianMutationmCherry sequence inserted just before the Stop co…Promoteradenovirus major late promoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT-ADARcd-E488Q-V5
Plasmid#139686PurposeExpresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5DepositorInsertAdenosine deaminase acting on RNA (Adar Fly)
UseTagsV5ExpressionBacterial and InsectMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
GSK3 alpha pMT2
Plasmid#15896DepositorInsertGSK3 alpha (GSK3A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2007AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMT-Diaphanous-GFP
Plasmid#24287DepositorInsertDiaphanous (dia Fly)
UseTagsGFPExpressionInsectMutationPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMT-Aurora B-GFP
Plasmid#24285DepositorInsertAurora B Kinase (aurB Fly)
UseTagsGFPExpressionInsectMutationPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMT-mCherry-Patronin
Plasmid#31753DepositorInsertPatronin (Patronin Fly)
UseTagsmCherryExpressionInsectMutationPromoterMetallothioneinAvailable sinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-UAP56 WT
Plasmid#110125PurposeExpresses Flag-tagged human UAP56 (DDX39B)DepositorInsertUAP56 (DDX39B Human)
UseTagsFlagExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT Shark-TagBFP
Plasmid#126658PurposeInducible expression of Shark-TagBFP in S2 cellsDepositorInsertShark
UseTagsExpressionInsectMutationPromoterAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+mEGFP-H1.4
Plasmid#157799PurposeExpression of mEGFP and human linker histone H1.4 fusion proteinDepositorInsertmEGFP-H1.4 (H1-4 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterpTACAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only