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We narrowed to 446 results for: DHFR

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Showing: 251 - 300 of 446 results

  1. pST50Trc1-HPCNDHFR

    Plasmid
    #63944
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Publication
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Protein C (HPC); TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63944-plasmid-map-sequence-id-177350

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  2. pST50Trc1-DHFRCYD

    Plasmid
    #63952
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Publication
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Covalent Yet Dissociable (CYD) [AGTEFCA]
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63952-plasmid-map-sequence-id-177358

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  3. pST50Trc1-HPCDHFR

    Plasmid
    #63939
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Publication
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Protein C (HPC)
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63939-plasmid-map-sequence-id-177345

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  6. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

    Plasmid
    #183410
    Purpose
    piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells
    Depositor
    Azim Surani
    Publication
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KRAB-dCas9-ecDHFR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    183410-plasmid-map-sequence-id-361256

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  17. pCyCEN_Lisp2mCherry_hsp70_GFP

    Plasmid
    #137169
    Purpose
    Dual fluorescent reporter for liver stage expression in Plasmodium cynomolgi
    Depositor
    Clemens Kocken
    Publication
    Voorberg-van der Wel et al Commun Biol. 2020 Jan 3;3:7. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    mCherry
    Nanoluc
    dihydrofolate reductase
    Green Fluorescent Protein
    Use
    Unspecified
    Tags
    T2A
    Promoter
    P. cynomolgi hsp70 and P. cynomolgi lisp2
    Available since
    May 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  24. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid
    #69534
    Purpose
    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.
    Depositor
    Timo Otonkoski
    Publication
    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DD-dCas9VP192-T2A-EGFP
    Tags
    C-term T2A-EGFP and DHFR Destabilised Domain
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    69534-plasmid-map-sequence-id-122831

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  27. MF82_PB-TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

    Plasmid
    #176626
    Purpose
    TF A self-activation cassette in MultiFate-2.2 and MultiFate-3
    Depositor
    Michael Elowitz
    Publication
    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.
    Insert
    TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
    Use
    Synthetic Biology
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176626-plasmid-map-sequence-id-347282

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  29. MF83_PB-TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

    Plasmid
    #176627
    Purpose
    TF B self-activation cassette in MultiFate-2.2 and MultiFate-3
    Depositor
    Michael Elowitz
    Publication
    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.
    Insert
    TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
    Use
    Synthetic Biology
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176627-plasmid-map-sequence-id-347070

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  30. pGEMS-II-dCas13-APO1-YTH

    Plasmid
    #204767
    Purpose
    GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)
    Depositor
    Kate Meyer
    Publication
    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas13-NLS-APO1-YTH-HA
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    Ef1alpha
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    204767-plasmid-map-sequence-id-408667

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  31. MF64_PB-TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

    Plasmid
    #176624
    Purpose
    TF B self-activation cassette in MultiFate-2.1 and MultiFate-2.3
    Depositor
    Michael Elowitz
    Publication
    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.
    Insert
    TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
    Use
    Synthetic Biology
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176624-plasmid-map-sequence-id-347134

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  32. MF85_PB-14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA

    Plasmid
    #176629
    Purpose
    TF C self-activation cassette in MultiFate-3
    Depositor
    Michael Elowitz
    Publication
    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.
    Insert
    14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA
    Use
    Synthetic Biology
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176629-plasmid-map-sequence-id-347269

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  37. pTUB1:YFP-AID-3HA, DHFR-TS:HXGPRT

    Plasmid
    #87260
    Purpose
    YFP-AID-3HA fusion driven by a minimal TUB1 promoter with an HXGPRT drug selectable marker. The AID CDS is Arabidopsis thaliana auxin-responsive protein IAA17.
    Depositor
    David Sibley
    Publication
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    YFP-AID-3HA
    HXGPRT
    Use
    Toxoplasma expression
    Tags
    3HA and AID
    Promoter
    TgDHFR-TS 463 bp and TgTUB1 378 bp
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    87260-plasmid-map-sequence-id-176554

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  40. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-ecDHFR-enOsCas12f1-NLS-ecDHFR_pA_AAV2 ITR

    Plasmid
    #197029
    Purpose
    AAV vector encoding a human codon-optimized ecDHFR-enOsCas12f1-ecDHFR driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6
    Depositor
    Xiangfeng Kong
    Publication
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized ecDHFR-enOsCas12f1-ecDHFR
    Use
    AAV
    Mutation
    D52R / T132R
    Promoter
    MHCK7, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197029-plasmid-map-sequence-id-388970

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  41. pCMV-EGFP-eDHFR(69K6)-MCS

    Plasmid
    #172100
    Purpose
    Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)
    Depositor
    Shinya Tsukiji
    Publication
    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-eDHFR(69K6)-20aa
    Expression
    Mammalian
    Mutation
    eDHFR(69K6): Hexalysine (K6) sequence is inserted…
    Promoter
    CMV
    Available since
    July 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    172100-plasmid-map-sequence-id-340527

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  48. pCMV-MCS-eDHFR(69K6)-EGFP

    Plasmid
    #172101
    Purpose
    Mammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFP
    Depositor
    Shinya Tsukiji
    Publication
    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    22aa-eDHFR(69K6)-EGFP
    Expression
    Mammalian
    Mutation
    eDHFR(69K6): Hexalysine (K6) sequence is inserted…
    Promoter
    CMV
    Available since
    July 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    172101-plasmid-map-sequence-id-340529

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Showing: 251 - 300 of 446 results