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pBetaActin-TUBB2a-mScarlet

Plasmid

#191324

Purpose

Expresses fluorescent labeled beta Tubulin to measure microtubule density

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mScarlet (Tubb2a Mouse)

Expression

Mammalian

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3_Csx29

Plasmid

#197606

Purpose

Expresses Csx29 in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

Csx29

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 VP4-VP2

Plasmid

#216787

Purpose

Mammalian expression of the VP4-VP2 portion of the CVB3 Kandolf strain polyprotein

Depositor

Kevin Janes

Article

Griffiths et al Sci Adv. 2024 Nov 15;10(46):eadp6303. do

Insert

VP4-VP2

Expression

Mammalian

Promoter

CMV

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pUltra-FPheKRS

Plasmid

#136631

Purpose

Genetic Incorporation 4-fluorophenyl carbamate lysine (FPheK) into proteins

Depositor

Han Xiao

Article

Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

Insert

FPheKRS

Expression

Bacterial

Promoter

Tac1 promoter

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-GB2

Plasmid

#125710

Purpose

Eukaryotic expression of the GB2 subunit of the metabotropic GABA receptor

Depositor

Kai Johnsson

Article

Masharina et al J Am Chem Soc. 2012 Nov 21;134(46):19026

Insert

GB2

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMARO

Plasmid

#178086

Purpose

Derivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.

Depositor

Sören Abel

Article

Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10

Insert

TcR (tetA/tetR)

Expression

Bacterial

Mutation

Tetracycline resistance cassette is flanked by Ec…

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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SUMO_TwinStrep_Csx30

Plasmid

#197611

Purpose

For protein purification of Csx30

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

SUMO_TwinStrep_Csx30

Expression

Bacterial

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT CVB3 A67G

Plasmid

#216788

Purpose

Gateway entry vector for the Kandolf strain of CVB3 with a VP4 A67G mutation

Depositor

Kevin Janes

Article

Griffiths et al Sci Adv. 2024 Nov 15;10(46):eadp6303. do

Insert

CVB3

Use

Entry vector for gateway cloning

Mutation

A67G in VP4

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-mNeonGreen

Plasmid

#191323

Purpose

Expresses fluorescent labeled beta Tubulin to measure microtubule density

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mNeonGreen (Tubb2a Mouse)

Expression

Mammalian

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJW1510

Plasmid

#62364

Purpose

pFA6a pSEC63-BirA-mVenus-UBC6-TA::HIS5

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA-mVenus-Ubc6 tail anchor

Expression

Yeast

Mutation

L232H mutation compared to standard mVenus

Promoter

Sec63

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pMLS719

Plasmid

#188894

Purpose

miniMos Arm sgRNA expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

PU6(K09B11.12)::miniMos Arm sgRNA

Expression

Worm

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJMP2480

Plasmid

#160080

Purpose

Z. mobilis CRISPRi, promoter C, empty guide

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

Nov. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Hm-epiA::TagRFP-T

Plasmid

#176430

Purpose

Drives expression of TagRFP-T under the control of Hofstenia partial epidermal marker (epiA) promoter

Depositor

Mansi Srivastava

Article

Ricci et al Dev Cell. 2021 Nov 22;56(22):3160-3170.e

Insert

tagRFP-T

Use

Transgenesis in hofstenia miamia

Promoter

Hofstenia miamia epiA gene promoter

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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Hm-psap::TagRFP-T

Plasmid

#176428

Purpose

Drives expression of TagRFP-T under the control of Hofstenia gut marker (psap) promoter

Depositor

Mansi Srivastava

Article

Ricci et al Dev Cell. 2021 Nov 22;56(22):3160-3170.e

Insert

tagRFP-T

Use

Transgenesis in hofstenia miamia

Promoter

Hofstenia miamia saposin 5

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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Xlone-tTA-42xControl

Plasmid

#245729

Purpose

Express 42xControl mRNA in mammalian cells

Depositor

Joshua Mendell

Article

Zhu et al Science. 2024 Nov 22;386(6724):eadq8587.

Insert

42xControl mRNA

Expression

Mammalian

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBetaActin-dCer3-C2-FRB

Plasmid

#191327

Purpose

Expresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-C2-dCer3

Expression

Mammalian

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-dCer3-C2-dCer3

Plasmid

#191328

Purpose

Expresses the fluorescently labeled membrane anchor C2 without the chemical dimerization domain FRB as a control for recruiting proteins to plasma membrane

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

dCer3-C2-dCer3

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FRB-dCer3-CAAX

Plasmid

#191326

Purpose

Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-dCer-CAAX

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-Dronpa

Plasmid

#191321

Purpose

Expresses fuorescently labeled beta tubulin to allow photoactivation of microtubule patches with very little UV light

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-Dronpa (Tubb2a Mouse)

Expression

Mammalian

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMLS713

Plasmid

#188885

Purpose

Multisite Gateway [4-3] Destination vector; miniMos transposon with lox2272-flanked Cbr-unc-119 marker

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Type

Empty backbone

Use

Multisite gateway [4-3] destination vector

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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