161,805 results

1. pCAGGS-ChR2-Venus

   Plasmid

   #15753

   Purpose

   Expresses ChR2-Venus for neuronal excitation.

   Depositor

   Karel Svoboda

   Article

   Petreanu et al Nat Neurosci. 2007 May . 10(5):663-8.

   Insert

   Channelrhodopsin2-Venus
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. DsRed-Rab5 DN

   Plasmid

   #13051

   Depositor

   Richard Pagano

   Article

   Sharma et al J Biol Chem. 2003 Feb 28. 278(9):7564-72

   Insert

   RAB5A (Canis familiaris) (RAB5A Canis familiaris)
   

   Use

   Tags

   DsRed2 and HA

   Expression

   Mammalian

   Mutation

   asparagine 3 changed to serine (neutral); aspara…

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe-tetoff-mVenus-PICdc42

   Plasmid

   #91876

   Purpose

   PI(WT): Photo inhibitable (blue light sensitive)

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Insert

   Vav2 (VAV2 Human)
   

   Use

   Tags

   mVenus

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. DsRed-Rab5 WT

   Plasmid

   #13050

   Depositor

   Richard Pagano

   Article

   Sharma et al J Biol Chem. 2003 Feb 28. 278(9):7564-72

   Insert

   RAB5A (Canis familiaris) (RAB5A Canis familiaris)
   

   Use

   Tags

   DsRed2 and HA

   Expression

   Mammalian

   Mutation

   asparagine 3 changed to serine (neutral); serine …

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAC-Luc

   Plasmid

   #33154

   Depositor

   Michael Rosbash

   Article

   Kadener et al Genes Dev. 2007 Jul 1;21(13):1675-86. Ep

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Insect

   Mutation

   Promoter

   Actin 5C

   Available since

   Dec. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pG1

   Plasmid

   #162602

   Purpose

   Edit Ade2 gene in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. GPR39-Tango

   Plasmid

   #66357

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR39 (GPR39 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEmpty_Control

   Plasmid

   #162607

   Purpose

   Empty control for Cas9 experiments and to clone new guide RNAs for paper

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC57-Amp_CRISPIE donor B5

   Plasmid

   #172846

   Purpose

   CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2+HyPer7

    Plasmid

    #136466

    Purpose

    Mammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWRS3001_VCR1_WCR3

    Plasmid

    #192206

    Purpose

    VCR1 and WCR3 tracrRNAs cloned into pTWIST AMP high copy backbone

    Depositor

    William Sellers

    Article

    Li et al Nat Commun. 2022 May 5;13(1):2469. doi:

    Insert

    VCR1-WCR3 tracrRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAGM51323

    Plasmid

    #153224

    Purpose

    Level 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 (Zcasi), and the nos terminator

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Insert

    Arabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330_DRS-1 sgRNA / hSpCas9

    Plasmid

    #172841

    Purpose

    Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Laconic/pcDNA3.1(-)

    Plasmid

    #44238

    Purpose

    a genetically-encoded sensor for lactate concentration, transport and metabolic flux

    Depositor

    Luis Felipe Barros

    Article

    San Martin et al PLoS One. 2013;8(2):e57712. doi: 10.1371

    Insert

    Laconic (LACtate Optical Nano Indicator from CECs)
    

    Use

    Lactate biosensor

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330_VCL sgRNA / hSpCas9

    Plasmid

    #172837

    Purpose

    Mammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRANS_210

    Plasmid

    #91108

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. CRISPR-StAR4 (Retro)

    Plasmid

    #222690

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    PGK without BbsI cutsite

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCSF107mT-GATEWAY-3'-Myc tag

    Plasmid

    #67617

    Purpose

    A pCS2 based vector to generate a 3'-Myc tag using GATEWAY cloning technology

    Depositor

    Todd Stukenberg

    Article

    Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

    Type

    Empty backbone

    Use

    In vitro transcription or translation off of an s…

    Tags

    C-terminal Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SP6

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. 15x-QUAS-dTomato-T2A-GCaMP6s

    Plasmid

    #130666

    Purpose

    QUAS reporter line that expresses cytosolic dTomato and the GCaMP6s calcium indicator, separated by T2A

    Depositor

    Leslie Vosshall

    Article

    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

    Insert

    15xQUAS-dTomato-T2A-GCaMP6s
    

    Use

    Ae. aegypti expression

    Tags

    Expression

    Insect

    Mutation

    Promoter

    15xQUAS-TATA

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-hATG7CS

    Plasmid

    #87868

    Purpose

    Express a active-site mutant human Atg7/Apg7 C572S in mammalian cells

    Depositor

    Isei Tanida, Eiki Kominami

    Article

    Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

    Insert

    ATG7 C572S mutant (ATG7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C572S mutant

    Promoter

    CMV

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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