59,428 results

1. pDEST-CMV GFP-LC3B WT

   Plasmid

   #123114

   Purpose

   Expresses EGFP-LC3B wild-type in mammalian cells. Fluorescent reporter for autophagosomes.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABE-puro-hTERT-HA

   Plasmid

   #1772

   Depositor

   Bob Weinberg

   Article

   Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

   Insert

   hTERT (TERT Human)
   

   Use

   Retroviral

   Tags

   2XHA

   Expression

   Mammalian

   Available Since

   June 3, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-Basic-Rab18-sfGFP(N) HDR template

   Plasmid

   #129415

   Purpose

   HDR tempalte for tagging of endogenous human RAB18 N-terminus with sfGFP

   Depositor

   Elina Ikonen

   Article

   Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

   Insert

   RAB18 HDR template (RAB18 Human)
   

   Use

   CRISPR and TALEN; Endogenous tagging hdr template

   Tags

   sfGFP

   Expression

   Mammalian

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-OsTIR1(F74G)

   Plasmid

   #140730

   Purpose

   OsTIR1(F74G)-P2A-mAID-EGFP-NES

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   OsTIR1(F74G)-P2A-mAID-EGFP-NES
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBabe-Kras G12D

   Plasmid

   #58902

   Purpose

   Expresses human Kras with a G12D Mutation

   Depositor

   Channing Der

   Insert

   KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   G12D mutation

   Available Since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-GFP-NMHC II-B

   Plasmid

   #11348

   Depositor

   Robert Adelstein

   Article

   Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

   Insert

   NMHC II-B (MYH10 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Feb. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-MSP1D1delltaH5

   Plasmid

   #71714

   Purpose

   Membrane scaffold protein (MSP), helix 5 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Tags

   6xHis and TEV site

   Expression

   Bacterial

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-NPC1(WT)-FLAG

   Plasmid

   #164972

   Purpose

   Lentiviral vector for NPC1 expression in mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

   Insert

   NPC1 (NPC1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5_mEGFP-NPM1

   Plasmid

   #237667

   Purpose

   For overexpression of mEGFP-NPM1

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   NPM1 (NPM1 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti MS2-P65-HSF1_Hygro

    Plasmid

    #61426

    Purpose

    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Insert

    MS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1A

    Available Since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223-ERBB2

    Plasmid

    #23888

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

12. HR180-LGR5-GFP

    Plasmid

    #129092

    Purpose

    KI EGFP with a puromycin/mRuby selection cassette into human LGR5 locus

    Depositor

    Toshiro Sato

    Article

    Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

    Insert

    EGFP and LGR5 homology arms (LGR5 Human)
    

    Use

    Homologous recombination donor plasmid

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Sense IRES

    Plasmid

    #69909

    Purpose

    Expresses a GFP circular RNA containing the sense EMCV IRES in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1, GFP (ZKSCAN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4.10/RSV_SF3B3-Xon_Luciferase

    Plasmid

    #174659

    Purpose

    Plasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.

    Depositor

    Beverly L. Davidson

    Article

    Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

    Insert

    Xon cassette
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Kozak ATG initiation sequence in middle exon

    Promoter

    RSV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. RMCE K>R DNA-PKcs

    Plasmid

    #233917

    Purpose

    Low copy number provides expression of human DNA-PKcs with a single K>R substitution that ablates kinase activity.

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    human DNA-PKcs kinase inactive mutant K3753>R (PRKDC Human)
    

    Expression

    Mammalian

    Mutation

    K3753R

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223-JAK2

    Plasmid

    #23915

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    JAK2 (JAK2 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. TRUPATH Triple (bidirectional) GasS

    Plasmid

    #196055

    Purpose

    Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphasS-RLuc8
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW-Flag-CtIP

    Plasmid

    #71108

    Purpose

    mammalian expession of Flag-CtlP

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    CtlP
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Please see Depositor Comments

    Promoter

    TRE promoter, Tet ON

    Available Since

    Nov. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pHAGE-PIK3CA-H1047R

    Plasmid

    #116500

    Purpose

    Lentiviral expression of PIK3CA H1047R

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Lentiviral

    Mutation

    H1047R

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. FUW-tetO-hOKMS

    Plasmid

    #51543

    Purpose

    Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2

    Depositor

    Tarjei Mikkelsen

    Article

    Cacchiarelli et al Cell. 2015 Jul 16;162(2):412-24. doi: 10

    Insert

    hOKMS (MYC, KLF4, SOX2, POU5F1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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