We narrowed to 4,881 results for: phy

1. VV209: sdChR(C138S E154A)-TS-GCaMP6f-ER in fck

   Plasmid

   #58524

   Purpose

   Expresses sdChR(C138S E154A) fused to GCaMP6f in mammalian cells

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   sdChR(C138S, E154A)-TS-GCaMP6f-ER
   

   Use

   Lentiviral

   Tags

   GCaMP6f

   Expression

   Mammalian

   Mutation

   changed Cysteine 138 to Serine; changed Glutamate…

   Promoter

   a-CamKII

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. attB-GFP

   Plasmid

   #65521

   Purpose

   Plasmid for the generation of GFP-knockin fusions via Bxb1-mediated recombination into MIN-tagged cell lines

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   attB-GFP
   

   Use

   Mouse Targeting; Bxb1

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLMB713

   Plasmid

   #83785

   Purpose

   Salicylic acid biosensor plasmid

   Depositor

   Philip Poole

   Article

   Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

   Insert

   Region upstream RL1329 (salA) containing all of RL1328 (salR)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Dendra2-Gamma-Tubulin-17

   Plasmid

   #57721

   Purpose

   Localization: Centrosomes, Excitation: 490 / 553, Emission: 507 / 573

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   Gamma-Tubulin (TUBG1 Human)
   

   Use

   Tags

   Dendra2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pRSF_6His-ZmSUMO1a-GG_ZmSCE1b-Myc

   Plasmid

   #91932

   Purpose

   Bacterial expression of maize SUMO1a and SCE1b

   Depositor

   Richard Vierstra

   Article

   Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

   Inserts

   6His-ZmSUMO1a-GG
   ZmSCE1b-Myc
   

   Use

   Tags

   6His and Myc

   Expression

   Bacterial

   Mutation

   Truncation of ZmSUMO1a after the diGlycine motif

   Promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDGB3_alpha1

   Plasmid

   #68228

   Purpose

   GoldenBraid level 1 Alpha plant expression vector.

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   empty vector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYFAC

   Plasmid

   #168982

   Purpose

   Filamentous fungi vector. Also referred to as pYFAC-pyrG.

   Depositor

   Yit Heng Chooi

   Article

   Chooi et al Environ Microbiol. 2017 May;19(5):1975-1

   Type

   Empty backbone

   Use

   Synthetic Biology; Heterologous expression in fil…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CROP_C9_Puro_PDGFRA

   Plasmid

   #183313

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets PDGFRA gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   PDGFRA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pORE303N_CEN12RRFuzzy3MYB

   Plasmid

   #194441

   Purpose

   CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12, RR, and Fuzzy3MYB

   Depositor

   Chung-Jui Tsai

   Article

   Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

   Insert

   gRNA array targeting CEN1, RR, Fuzzy3MYB
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   MtU6

   Available since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDsRed (GB0100)

    Plasmid

    #68202

    Purpose

    Provides the CDS of DsRed as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    DsRed coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CROP_C9_Puro_JAK2

    Plasmid

    #183303

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets JAK2 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    JAK2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SLF091: Lenti-CaMKIIa-eTsChR-eGFP

    Plasmid

    #112281

    Purpose

    For lentiviral or direct expression of eTsChR in excitatory neurons

    Depositor

    Adam Cohen

    Article

    Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

    Insert

    eTsChR
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mOrange-H2B-6

    Plasmid

    #57942

    Purpose

    Localization: Nucleus/Histones, Excitation: 548, Emission: 562

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mOrange

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pRSF_6His-ZmSUMO1a-GG_ZmSCE1d-Myc

    Plasmid

    #91934

    Purpose

    Bacterial expression of maize SUMO1a and SCE1d

    Depositor

    Richard Vierstra

    Article

    Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

    Inserts

    6His-ZmSUMO1a-GG
    ZmSCE1d-Myc
    

    Use

    Tags

    6His and Myc

    Expression

    Bacterial

    Mutation

    Truncation of ZmSUMO1a after the diGlycine motif

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSW178 - pXPP

    Plasmid

    #115987

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Alexis Maizel

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pXPP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mOrange2-H2B-6

    Plasmid

    #57962

    Purpose

    Localization: Nucleus/Histones, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

17. BK beta1

    Plasmid

    #113565

    Purpose

    mouse BK beta1 subunit in mammalian expression vector

    Depositor

    Robert Brenner

    Article

    Wang et al J Gen Physiol. 2006 Dec;128(6):731-44. d

    Insert

    Kcnmb1 (Kcnmb1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pME18S-3HA-mULK1(K46N)

    Plasmid

    #22897

    Depositor

    Masaaki Muramatsu

    Article

    Yan et al Biochem Biophys Res Commun. 1998 May 8.

    Insert

    a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    changed Lysine 46 to Aspargine

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

19. Danio rerio cryabb

    Plasmid

    #47665

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha B-crystallin (cryabb Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AEB-F12

    Plasmid

    #63849

    Purpose

    This pFTV1 vector contains a fluoride binding riboswitch, controlling the translation initaition rate of mRFP1 gene.

    Depositor

    Howard Salis

    Article

    Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

    Insert

    F12 fluoride riboswitch
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-HA-ERK2 L232A

    Plasmid

    #8976

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L232A

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

22. pUM31dao

    Plasmid

    #87242

    Purpose

    Plastid expression cassette containing Schizosaccharomyces pombe D-amino acid oxidase gene. Provides dual positive (D-alanine) and negative (D-valine) selection in transplastomic plants

    Depositor

    Anil Day

    Article

    Gisby et al Plant Physiol. 2012 Dec;160(4):2219-26.

    Insert

    D-amino oxidase
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    plastid rrn

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBS-beta1,4 GT-1

    Plasmid

    #11872

    Depositor

    Michiko Fukuda

    Article

    Masri et al Biochem Biophys Res Commun. 1988 Dec 15.

    Insert

    beta 1,4 galactosyltransferase (B4GALT1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

24. pcDNA3.1-6His-MyoX

    Plasmid

    #47607

    Depositor

    Emanuel Strehler

    Article

    Bennett et al Biochem Biophys Res Commun. 2008 May 2;3

    Insert

    MyoX (MYO10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q680R

    Promoter

    CMV

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Dendra2-Keratin-17

    Plasmid

    #57726

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

26. VV201: ChR2(C128S)-eGFP in fubi

    Plasmid

    #58510

    Purpose

    Expresses channelrhodopsin-2 with the C128S (step function opsin) mutation fused to eGFP under the ubiquitin promoter

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    ChR2(C128S)-eGFP
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Changed Cysteine 128 to Serine

    Promoter

    Ubiquitin

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MAC_C_INSR

    Plasmid

    #187788

    Purpose

    MAC-tagged gene expression of human INSR

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    INSR (INSR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-HA-ERK2 Y261A

    Plasmid

    #8977

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y261A

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

29. pLJC6-NADK-3xFLAG

    Plasmid

    #163452

    Purpose

    lentiviral overexpression vector for human NADK

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    NADK (NADK Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    See: comments

    Promoter

    Ubc

    Available since

    March 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CROP_C9_Puro_VEGFB

    Plasmid

    #183328

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets VEGFB gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    VEGFB
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CROP_C9_Puro_VEGFA

    Plasmid

    #183327

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets VEGFA gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    VEGFA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. CROP_C9_Puro_PDGFRB

    Plasmid

    #183314

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets PDGFRB gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    PDGFRB
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAL189

    Plasmid

    #22278

    Purpose

    Expresses Intersectin DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    IntersectinDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMOS010E: Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)

    Plasmid

    #163063

    Purpose

    Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    CheRiff
    

    Use

    Gateway entry vector

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. mEos2-H1-10

    Plasmid

    #57383

    Purpose

    Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    H1 (H1f0 Mouse)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. mEos2-Calnexin-N-14

    Plasmid

    #57352

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Calnexin (CANX Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    I474N in Calnexin

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

37. mEos2-ER-5

    Plasmid

    #57373

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    ER (CALR Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    ER Signal Peptide

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

38. pC0.167

    Plasmid

    #119636

    Purpose

    Level 0 Part. Insertion site

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    6803 NS2 Up Flank
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    non functional/large non-specific insertion

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pC0.168

    Plasmid

    #119635

    Purpose

    Level 0 Part. Insertion site

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    6803 NS2 Down Flank
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    bp 630 A to T

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pVL23 - pVND7

    Plasmid

    #116019

    Purpose

    entry vector for GreenGate cloning method, promoter

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pVND7
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge