We narrowed to 9,944 results for: Gnas

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pCMV5-ALK2 K235R

Plasmid

#11755

Depositor

Jeff Wrana

Article

Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

Insert

ALK2 (ACVR1 Human)

Expression

Mammalian

Mutation

K235R: kinase dead

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits
GST-RP2

Plasmid

#86072

Purpose

A bacterial expression plasmid encoding retinitis pigmentosa 2 (RP2) with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

RP2 (RP2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-PRAS40

Plasmid

#15477

Depositor

Do-Hyung Kim

Article

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

PRAS40 shRNA (AKT1S1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-optoB2-LYY-YFP

Plasmid

#75271

Purpose

G-protein independent version of optoB2 with point mutations at L72F,Y136G,Y224A

Depositor

Michael Bruchas

Article

Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

Insert

G-protein independent version of optoB2

Tags

eYFP

Expression

Mammalian

Mutation

point mutations at L72F,Y136G,Y224A

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP-PKCg-C1

Plasmid

#112266

Purpose

ITRACKgamma Donor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

protein kinase c gamma (Prkcg Mouse)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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px330 RAD21 gRNA

Plasmid

#165592

Purpose

Insertion of RAD21 degron

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

RAD21 (Rad21 Mouse)

Expression

Mammalian

Mutation

NA

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pJH3185

Plasmid

#132367

Purpose

Prgef-1 GFP::DAF-16a unc-54 3' UTR C.elegans pan-neural expression of isoform a of DAF-16 fused to GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-16a (daf-16 Nematode)

Tags

GFP

Expression

Worm

Promoter

Prgef-1

Available Since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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FCIV1-GR

Plasmid

#72701

Purpose

Lentiviral expression of rat GR (2nd generation)

Depositor

Freddy Jeanneteau

Article

Lambert et al Mol Cell Biol. 2013 Sep;33(18):3700-14.

Insert

GR (Nr3c1 Rat)

Use

Lentiviral

Tags

IRES-Venus

Expression

Mammalian

Promoter

ubiquitin

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
pSicoR (Puro) p65 shRNA2

Plasmid

#22508

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

p65 shRNA2 (Rela Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only
pNICE HA-mSYD1A

Plasmid

#59361

Purpose

Expression plasmid for HA-tagged mSYD1A / Syde1 (HA-tag at N-terminus of mSYD1A)

Depositor

Peter Scheiffele

Article

Wentzel et al Neuron. 2013 Jun 19;78(6):1012-23. doi:

Insert

mSYD1A (Syde1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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CALNL-RARa-VP16

Plasmid

#185560

Purpose

Cre Dependent ubiquitous expression of constitutively active Retinoic Acid Receptor alpha

Depositor

Connie Cepko

Article

Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

Insert

RARa-VP16 (Rara Mouse)

Tags

VP16

Expression

Mammalian

Promoter

CAG

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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PECAM-H2B-GFP-Hygro

Plasmid

#14684

Depositor

Victoria Bautch

Article

Zeng et al Blood. 2007 Feb 15. 109(4):1345-52.

Insert

PECAM promoter/enhancer (Pecam1 Mouse)

Tags

H2B-GFP and PGK-Hygro

Expression

Mammalian

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only
pMM585-Tier1-PhCMV-Igk

Plasmid

#169486

Purpose

Tier-1 vector encoding PhCMV-driven secretion signal Igk (PhCMV-Igk-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven Igk secretion signal (IGK Human)

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-CDX2-MUT

Plasmid

#16554

Depositor

Bert Vogelstein

Article

da Costa et al Oncogene. 1999 Sep 2. 18(35):5010-4.

Insert

CDX2 (CDX2 Human)

Use

Adenoviral

Expression

Mammalian

Mutation

RKO mutation

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only
FL-fibulin-3 pcDNA4

Plasmid

#29700

Depositor

Mariano Viapiano

Article

Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

Insert

EFEMP1 (EFEMP1 Human)

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pNLRP11major.MYC

Plasmid

#131196

Purpose

Mammalian expression of human NLRP11 188S

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

NLRP11 188S (NLRP11 Human)

Tags

myc

Expression

Mammalian

Mutation

188S

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-Arl13b1

Plasmid

#40870

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Gateway entry

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt11-V5

Plasmid

#35941

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt11 (wnt11f2 Human)

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-ERK2 Y261A

Plasmid

#8977

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Tags

HA

Expression

Mammalian

Mutation

Y261A

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-PM-CaNARi

Plasmid

#64850

Purpose

Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

Lyn-CaNARi

Tags

Cerulean, N-terminal targeting sequence from Lyn …

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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GL2 shRNA

Plasmid

#86083

Purpose

A lentiviral construct for building stable cell lines expressing GL2 shRNA via puromycin selection.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

control shRNA

Promoter

U6

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Wnt9B

Plasmid

#35919

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt9B (WNT9B Human)

Expression

Mammalian

Mutation

SNP Met106-->Thr

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mSmo-WT-V5

Plasmid

#126415

Purpose

Localization, Expression of Smoothened WT in mammalian cell lines.

Depositor

Stacey Ogden

Article

Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

Insert

m-Smoothened (Smo Mouse)

Expression

Mammalian

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK2 L232A

Plasmid

#8976

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Tags

HA

Expression

Mammalian

Mutation

L232A

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pNICE HA-mSYD1B

Plasmid

#59362

Purpose

Expression plasmid for HA-tagged mSYD1B / Syde2 (HA-tag at N-terminus of mSYD1B)

Depositor

Peter Scheiffele

Article

Wentzel et al Neuron. 2013 Jun 19;78(6):1012-23. doi:

Insert

mSYD1B (Syde2 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRE1 CTF-FLAG

Plasmid

#217689

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRE1 (EMR1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-584

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRF.1-mCMV-udsVenus

Plasmid

#58518

Purpose

Empty lentiviral expression vector for ultradestabilized Venus that indicates the activity of a transcriptional element of interest

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

None

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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Beta-arrestin2 K11,12R GFP

Plasmid

#35412

Depositor

Robert Lefkowitz

Article

Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

Insert

βarrestin2 (Arrb2 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

K11,12R

Promoter

CMV

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only
Pinco-mNrp1dCy

Plasmid

#21936

Depositor

Guido Serini

Article

Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

Insert

neuropilin 1 (Nrp1 Mouse)

Use

Retroviral

Tags

HA and His

Mutation

deleted amino acids 884-923

Available Since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
Pinco-mNrp1dSEA

Plasmid

#21935

Depositor

Guido Serini

Article

Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

Insert

neuropilin 1 (Nrp1 Mouse)

Use

Retroviral

Tags

HA and His

Mutation

deleted amino acids 920-923

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pHES609 [prNOp7-PBAC]

Plasmid

#103017

Purpose

A S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA.

Depositor

Hana El-Samad

Article

Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

Insert

bPAC

Expression

Yeast

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
XE68 APC-GFP in CS2+

Plasmid

#16687

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

APC-GFP (APC Human)

Use

Xenopus expression

Tags

GFP

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
pCS2 GFP-GSK3-MAPK-E3mut

Plasmid

#29691

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

GFP glycogen synthase kinase 3 recognition site E3 ligase site mutated primed by MAPK

Tags

Flag and GFP

Expression

Mammalian

Mutation

recognition sites only, and E3 ligase site mutated

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt2-V5

Plasmid

#35925

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt2 (WNT2 Human)

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation G (816) --> A

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
aq-CFP

Plasmid

#36073

Depositor

Narasimhan Gautam

Article

Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

Insert

G protein subunit alpha q (Gnaq Mouse)

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted between amino acid 124 and 125

Promoter

CMV

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only
pCS2 Flag Smad3 S204A

Plasmid

#27114

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

S204A

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
coreR1 (384-1008)

Plasmid

#115646

Purpose

expression of truncated Rag1

Depositor

Martin Gellert

Article

Grundy et al Proc Natl Acad Sci U S A. 2010 Dec 28;10

Insert

Rag1 core (384-1008) (Rag1 Mouse)

Tags

8XHis-MBP-PreScission

Expression

Mammalian

Mutation

truncated Rag1 (aa 384-1008)

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
VYAIFIFQL_epitope

Plasmid

#165018

Purpose

Expression of CMV US20 (29-37) fused to IL-2 signal sequence

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

CMV US20 (29-37)

Use

Lentiviral

Tags

human IL2 signal sequence at N-term

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
human syndecan 2 EFYA

Plasmid

#64971

Purpose

Constitutive expression in mammalian cells

Depositor

Enric Espel

Article

Rovira-Clave et al Cell Signal. 2014 Jul;26(7):1489-99. doi

Insert

syndecan 2 (SDC2 Human)

Expression

Mammalian

Mutation

deleted EFYA

Promoter

CMV

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only
human syndecan 2 FAAF

Plasmid

#64972

Purpose

Constitutive expression in mammalian cells

Depositor

Enric Espel

Article

Rovira-Clave et al Cell Signal. 2014 Jul;26(7):1489-99. doi

Insert

syndecan 2 (SDC2 Human)

Expression

Mammalian

Mutation

Y179F, S187A, S188A, Y191F

Promoter

CMV

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 AT1R DRY-AAY

Plasmid

#45759

Depositor

Laszlo Hunyady, Robert Lefkowitz

Article

Gaborik et al Endocrinology. 2003 Jun;144(6):2220-8.

Insert

AT1R DRY-AAY (Agtr1a Rat)

Tags

HA

Expression

Mammalian

Mutation

changed amino acids 125-126 from DR to AA

Promoter

CMV

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-PH-GAP1IP4BP

Plasmid

#20200

Depositor

Robin Irvine

Article

Hammond et al J Cell Biol. 2009 Jan 26;184(2):297-308.

Insert

PH-GAP1IP4BP (RASA3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Isolated PH/Btk domain

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pAct5C-Hh

Plasmid

#37382

Depositor

Norbert Perrimon

Article

Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub

Insert

hedgehog (hh Fly)

Expression

Insect

Promoter

actin5C

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
Lyn-FRB-GFP11

Plasmid

#181863

Purpose

Plasma membrane-localized GFP11; for plasma membrane targeting of FluoSTEPs.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

Lyn-FRB-GFP11

Tags

GFP11 and N-terminal targeting sequence from Lyn …

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Ilmol

Plasmid

#120310

Purpose

A red-shifted GEVI consists of triple mutant ciona based voltage sensing domain with optimized linker and dTomato K159R.

Depositor

Bradley Baker

Article

Yi et al Sci Rep. 2018 Oct 12;8(1):15199. doi: 10

Insert

Ilmol

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101107

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

BDKRB2-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
RnAT1aR-mCherry

Plasmid

#137782

Purpose

Visualization of At1aR

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat AT1a receptor (Agtr1a Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
BAT-lux

Plasmid

#20890

Depositor

Stefano Piccolo

Article

Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

Insert

7xTCF binding sites, Siamois minimal promoter

Use

Luciferase

Expression

Mammalian

Mutation

7 multimerized TCF-binding elements fused upstrea…

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
pmGFP

Plasmid

#188451

Purpose

Expression of a modified GFP (mGFP) with short half-life

Depositor

Ajit Chande

Article

Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

Insert

Cyclin destruction box

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG-Fos AA

Plasmid

#8967

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S362A, S374A

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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We narrowed to 9,944 results for: Gnas