Addgene

We narrowed to 9,181 results for: Coli

Showing: 3051 - 3100 of 9181 results

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pBEL2092

Plasmid

#162137

Purpose

Expression and Complementation plasmid for MlaE IF1 1-30 aa deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Use

Tags

Expression

Bacterial

Mutation

MlaF-MlaE(delta1-30)-(6xHis-MlaD)-MlaC-MlaB

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2133

Plasmid

#162138

Purpose

Expression and Complementation plasmid for MlaE IF1 1-39 aa deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Use

Tags

Expression

Bacterial

Mutation

MlaF-MlaE(delta1-39)-(6xHis-MlaD)-MlaC-MlaB

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Avs4_Chimera

Plasmid

#188802

Purpose

Chimeric Avs4 with transmembrane effector

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Avs4 chimera

Use

Tags

Expression

Bacterial

Mutation

Promoter

EcAvs4 native promoter

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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p-preSufI(IAC)

Plasmid

#168516

Purpose

Modified form of pre-SufI in pET25b. With C-terminal HHHHHHC (6xHisC) extension and mutations C17I and C295A.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

pre-SufI(IAC)

Use

Tags

6xHisC tag and HSV tag

Expression

Bacterial

Mutation

C17I and C295A

Promoter

T7

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pEc-crRNA2

Plasmid

#170089

Purpose

encodes E. coli type I-E single spacer array

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E single spacer array

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

J23119

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2039

Plasmid

#139929

Purpose

Complementation plasmid for LetAB without LetB TM region

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(43-877)-6xHis

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2005

Plasmid

#139880

Purpose

Complementation plasmid for LetAB with 2 ring (rings17) LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(delta161-747)

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1993

Plasmid

#139897

Purpose

Expression plasmid for LetB PLL1 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta114-128, P113G)-6xHis

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1999

Plasmid

#139903

Purpose

Expression plasmid for LetB PLL7 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta816-826, S815G, T827G)-6xHis

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pHIS8-3_DL121

Plasmid

#171953

Purpose

Expression vector for ecDHFR-asLOV2 fusion protein. Also has TYMS

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Insert

Fusion of ecDHFR and asLOV2 at position 121 of DHFR

Use

Tags

8-His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pKB237.1

Plasmid

#170031

Purpose

Encodes LacI-controlled expression of surface-displayed CCL19 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
CCL19 Δ alpha-helix

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Mutation

Promoter

Ptac and PvirK

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pKP381

Plasmid

#167592

Purpose

Express TonB H20A protein

Depositor

Kathleen Postle

Article

Larsen et al J Bacteriol. 2007 Apr;189(7):2825-33. do

Insert

tonB H20A

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pVER-LacI

Plasmid

#164830

Purpose

LacI protein regulates expression of yellow fluorescent protein. For measuring dose-response curves.

Depositor

Drew Tack

Article

Tack et al Mol Syst Biol. 2021 Mar;17(3):e10179. do

Insert

LacI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Ptac

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2298

Plasmid

#164603

Purpose

Expression and Complementation plasmid for MlaD (A17F, A20F, V24F)

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Use

Tags

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(Ala17Phe, Ala20Phe, Val24P…

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTY1-LacI

Plasmid

#164831

Purpose

LacI protein regulates the expression of a tetracycline resistance gene and yellow fluorescent protein

Depositor

Drew Tack

Article

Tack et al Mol Syst Biol. 2021 Mar;17(3):e10179. do

Insert

LacI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Ptac

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1647

Plasmid

#155148

Purpose

Expresses MlaF 1-246, tagless

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)

Use

Tags

Expression

Mutation

Promoter

araBAD

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1840

Plasmid

#155150

Purpose

Expresses MlaF-6xHis

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-6xHis

Use

Tags

Expression

Mutation

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-09

Plasmid

#158437

Purpose

pBAD33 vector encoding MotA, Dendra2 - GSGSGS linker - MotB

Depositor

Francesco Pedaci

Article

Heo et al Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.

Inserts

MotA
MotB

Use

Tags

Dendra2- 3 x EAAAK linker

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-25

Plasmid

#158436

Purpose

pBAD33 vector encoding MotA, YPet - 3 x EAAAK linker - MotB

Depositor

Francesco Pedaci

Article

Heo et al Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.

Inserts

MotA
MotB

Use

Tags

yPET - 3 x EAAAK linker

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMH-05

Plasmid

#158435

Purpose

pBAD33 vector encoding MotA, YPet - EAAAK linker - MotB

Depositor

Francesco Pedaci

Article

Heo et al Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.

Inserts

MotA
MotB

Use

Tags

yPET - EAAAK linker

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1889

Plasmid

#139893

Purpose

Complementation plasmid for LetAB with LetB PLL4 deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(delta459-360)

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1996

Plasmid

#139900

Purpose

Expression plasmid for LetB PLL4 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta459-360)-6xHis

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2041

Plasmid

#139926

Purpose

Complementation plasmid for LetAB with 4 ring (rings 1237) LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB (1-391; 747-877)

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1892

Plasmid

#139896

Purpose

Complementation plasmid for LetAB with LetB PLL7 deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(delta816-826, S815G, T827G)

Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2040

Plasmid

#139930

Purpose

Complementation plasmid for LetAB with LetB TM region replaced by PelB leader sequence

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-(PelB-LetB(43-877))-6xHis

Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2071

Plasmid

#139928

Purpose

Complementation plasmid for LetAB operon with LetA and LetB genes deoverlapped

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB_deoverlapped

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1859

Plasmid

#139906

Purpose

Complementation plasmid for LetAB with LetB mutant V247N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(V247N)

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1858

Plasmid

#139905

Purpose

Complementation plasmid for LetAB with LetB mutant L246N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(L246N)

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1890

Plasmid

#139894

Purpose

Complementation plasmid for LetAB with LetB PLL5 deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(delta581-590, K580G, Q591G)

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2044

Plasmid

#139923

Purpose

Expression plasmid for 4 ring (rings 1237) LetB (without TM region) mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB (43-391; 747-877)

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1731

Plasmid

#139922

Purpose

Expression plasmid for LetB mutant E854Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; E854Amber)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1730

Plasmid

#139921

Purpose

Expression plasmid for LetB mutant K488Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; K488Amber)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1729

Plasmid

#139920

Purpose

Expression plasmid for LetB mutant F833Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; F833Amber)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1728

Plasmid

#139919

Purpose

Expression plasmid for LetB mutant F818Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; F818Amber)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1727

Plasmid

#139918

Purpose

Expression plasmid for LetB mutant Y814Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; Y814Amber)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1726

Plasmid

#139917

Purpose

Expression plasmid for LetB mutant W476pa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; W476Amber)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1725

Plasmid

#139916

Purpose

Expression plasmid for LetB mutant F468Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; F468Amber)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1811

Plasmid

#139915

Purpose

Expression plasmid for LetB mutant L358N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; L359N)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1810

Plasmid

#139914

Purpose

Expression plasmid for LetB mutant L358N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; L358N)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1809

Plasmid

#139913

Purpose

Expression plasmid for LetB mutant L355N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; L355N)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1808

Plasmid

#139912

Purpose

Expression plasmid for LetB mutant V247N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; V247N)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1807

Plasmid

#139911

Purpose

Expression plasmid for LetB mutant L246N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; L246N)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1806

Plasmid

#139910

Purpose

Expression plasmid for LetB mutant L243N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; L243N)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1862

Plasmid

#139909

Purpose

Complementation plasmid for LetAB with LetB mutant L359N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(L359N)

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1861

Plasmid

#139908

Purpose

Complementation plasmid for LetAB with LetB mutant L358N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(L358N)

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1860

Plasmid

#139907

Purpose

Complementation plasmid for LetAB with LetB mutant L355N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(L355N)

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1857

Plasmid

#139904

Purpose

Complementation plasmid for LetAB with LetB mutant L243N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(L243N)

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1998

Plasmid

#139902

Purpose

Expression plasmid for LetB PLL6 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta703-708, Q702G, E709G)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1997

Plasmid

#139901

Purpose

Expression plasmid for LetB PLL5 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta581-590, K580G, Q591G)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1995

Plasmid

#139899

Purpose

Expression plasmid for LetB PLL3 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta347-360, P346G)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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