We narrowed to 2,348 results for: clu

1. pLViN-iRFP670-α-cateninA+

   Plasmid

   #229703

   Purpose

   Lentiviral expression of iRFP670-alpha-cateninA+ in mammalian cells

   Depositor

   Alpha Yap

   Article

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
   

   Use

   Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   amino acids 670-673, RAIM--> GSGS

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2)

   Plasmid

   #169160

   Purpose

   To express nsp14-nsp10 fusion protein in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   14His-SUMO-nsp14-GGSGGS-nsp10 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   14His-SUMO and Fusion protein of SARS-CoV-2 nsp14…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST-Myc-FBW7 Delta F-Box

   Plasmid

   #197452

   Purpose

   The plasmid expresses F-box deleted version of Myc-tagged FBW7 human isoform 1. Used for mammalian overexpression of FBW7alpha delta-F-Box and detection by western blotting.

   Depositor

   Omar Khan

   Article

   Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

   Insert

   FBW7 (FBXW7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   FBW7 Fbox deleted.

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. DNA-CARzeta-GFP

   Plasmid

   #89344

   Purpose

   2nd generation lentiviral vector which expresses DNA-CAR receptor fused to GFP

   Depositor

   Ron Vale

   Article

   Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

   Insert

   DNA-CARzeta (CD247, CD86 Human)
   

   Use

   Lentiviral

   Tags

   SNAPf tag and mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEMS1951

   Plasmid

   #105868

   Purpose

   High copy number plasmid containing Hspa1a (synonym Hsp68) MiniPromoter insert

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Insert

   Hspa1a (synonym Hsp68) MiniPromoter (HSPA1A, HSPA1A Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Hsp68 MiniPromoter

   Available since

   Feb. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pmEos2-N1 GBP (GBP-mEos2)

   Plasmid

   #162876

   Purpose

   Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy

   Depositor

   Frederic Meunier

   Article

   Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

   Insert

   GFP Binding Protein tagged with mEos2
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. dCas9-CBP HAT

   Plasmid

   #179547

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET11a_3xFlag-nsp14 (SARS-CoV-2)

   Plasmid

   #169159

   Purpose

   To express SARS-CoV-2 nsp14 in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T7

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4.23 C3_7

   Plasmid

   #60293

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   SLC30A8 enhancer (SLC30A8 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pLXSN p200 CUX1

    Plasmid

    #90470

    Purpose

    Retroviral vector expressing human p200 CUX1 with a Myc and HA tag at the N- and C-terminus, respectively

    Depositor

    Alain Nepveu

    Article

    Kedinger et al J Biol Chem. 2009 Oct 2;284(40):27701-11

    Insert

    CUX1 (CUX1 Human)
    

    Use

    Retroviral

    Tags

    Ha and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Moloney murine leukemia virus long terminal repeat

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE2-TetOminiCMV-SK

    Plasmid

    #136577

    Purpose

    Dox-inducible polycistronic lentiviral vector expressing mouse Sox2, Klf4

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Sox2-T2A-Klf4 (Klf4, Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP

    Plasmid

    #176279

    Purpose

    Viral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    Kir2.1MUT-P2A-EGFP (Kcnj2 Mouse, Synthetic)
    

    Use

    AAV and Cre/Lox

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    GYG to AAA (aa144-146)

    Promoter

    human Synapsin I

    Available since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR D-TOPO-C3_9

    Plasmid

    #60258

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    NKX6-1 enhancer (NKX6-1 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4.23 C3_25

    Plasmid

    #60305

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    ROCK1 enhancer (ROCK1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pET11a_3xFlag-nsp10 (SARS-CoV-2)

    Plasmid

    #169157

    Purpose

    To express SARS-CoV-2 nsp10 in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    3xFlag-nsp5CS[VRLQ]-nsp10 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    3xFlag and nsp5CS[VRLQ]: predicted nsp5 protease …

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRI001-pGEM-LS-Bar-PgpdA-dLbCas12a-VPR-Ttrpc-LS

    Plasmid

    #140193

    Purpose

    Chromosomal integration of PgpdA-dLbCas12a-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi integrative vector.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Inserts

    dLbCas12a-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    

    Use

    CRISPR and Synthetic Biology; Fungal genome integ…

    Tags

    VPR (VP64-p65-Rta), NLS

    Expression

    Mutation

    D832A DNAse deactivated

    Promoter

    PgpdA and PtrpC

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)

    Plasmid

    #169164

    Purpose

    Baculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp14/nsp10-6His-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    6His-3xFlag (nsp10 C-terminus)

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL4.23 C3_9

    Plasmid

    #60295

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    NKX6-1 enhancer (NKX6-1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pEMS1401

    Plasmid

    #29198

    Purpose

    High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP reporter

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    Ple67 (FEV Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. t206-405

    Plasmid

    #166132

    Purpose

    For expression of human talin-1 head fragment (residues 206-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

    Depositor

    Vesa Hytönen

    Article

    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

    Insert

    T1head1-F2F3(206-405) (TLN1 Human)
    

    Use

    Tags

    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

    Expression

    Bacterial

    Mutation

    residues 206-405 only

    Promoter

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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