162,698 results

1. pHelper_ShCAST_sgRNA

   Plasmid

   #127921

   Purpose

   Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEG302 22aa SunTag VP64 nog

   Plasmid

   #120251

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-RIα-EGFP

   Plasmid

   #181840

   Purpose

   GFP-tagged PKA RIα subunit for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   RIα-EGFP (PRKAR1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Multi-frame tag

   Plasmid

   #128610

   Purpose

   In the Multi-frame tag, HIV-1 frameshift sequence is upstream of FLAG epitopes in the 0 frame are separated from one another by SunTag epitopes in the -1 frame.

   Depositor

   Tim Stasevich

   Article

   Lyon et al Mol Cell. 2019 Jul 11;75(1):172-183.e9.

   Insert

   MF tag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCherry.90 alpha

   Plasmid

   #108222

   Purpose

   Mammalian expression vector for mCherry fusion to human Hsp90 alpha

   Depositor

   Didier Picard

   Article

   Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

   Insert

   mCherry-Hsp90a (HSP90AA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LZRS-DNMT1

   Plasmid

   #24952

   Depositor

   Paul Khavari

   Article

   Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

   Insert

   DNMT1 (DNMT1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-GST

   Plasmid

   #42049

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   GST
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mARGAna (cassette 1)

   Plasmid

   #191341

   Purpose

   Second-generation mammalian acoustic reporter gene derived from Anabaena

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   mARGAna (cassette 1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GV

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKDsgRNA-NT

   Plasmid

   #89960

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   empty gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTW2154

    Plasmid

    #236578

    Purpose

    ISYmu1 CHLl1 gRNA4 TRV2 Architecture_B

    Depositor

    Steven Jacobsen

    Article

    Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

    Insert

    ISYmu1 TnpB
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACYCDuet-1_Ec_rpoZ

    Plasmid

    #128837

    Purpose

    E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

    Depositor

    Seth Darst

    Article

    Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

    Insert

    rpoZ (rpoZ E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. NW9

    Plasmid

    #133442

    Purpose

    Covalently circularized nanodisc NW9 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP-Aurora A

    Plasmid

    #198402

    Purpose

    Expression of EGFP-tagged Aurora A in mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

    Insert

    Aurora A
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT3TS-Tol2

    Plasmid

    #31831

    Depositor

    Stephen Ekker

    Article

    Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

    Insert

    Tol2 Transposase
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Lac

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRX-IPU-GFP-MYH9

    Plasmid

    #168273

    Purpose

    Expresses MYH9 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

    Insert

    MYH9 (MYH9 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTXB1-pA-M.EcoGII

    Plasmid

    #192873

    Purpose

    pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578

    Depositor

    Chong Tang

    Article

    Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:

    Insert

    pA-M.EcoGII
    

    Use

    Tags

    3X Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK231 (AAVS1 CMV-MCS-PURO)

    Plasmid

    #105924

    Purpose

    Donor for integration at the human AAVS1 locus

    Depositor

    Masato Kanemaki

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX_hDDX5_FL_wt

    Plasmid

    #88871

    Purpose

    inducible expression of GST-DDX5 in bacteria

    Depositor

    Dan Littman

    Article

    Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-evoCAST-evoTnsC

    Plasmid

    #234730

    Purpose

    Evolved human-codon optimized PseTnsC in mammalian cells (for evoCAST)

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    evoPseTnsC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJRH-1328 LV EF1a-CD20 IRES-EGFP

    Plasmid

    #201918

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD20

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD20
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge