169,334 results

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Flag-HRD1

Plasmid

#231688

Purpose

Expresses human HRD1 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

HRD1 (SYVN1 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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NG-ABE8e

Plasmid

#138491

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSpCas9 (NG)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-LRRK2

Plasmid

#232872

Purpose

Expresses LRRK2 in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

GFP-LRRK2 (LRRK2 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a-alpha-synuclein

Plasmid

#51486

Purpose

Inducible expression of human alpha-synuclein in E.coli

Depositor

Michael J Fox Foundation MJFF

Insert

alpha-synuclein (SNCA Human)

Expression

Bacterial

Promoter

T7

Available Since

March 31, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV R-CEPIA4mt

Plasmid

#140463

Purpose

Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Depositor

Masamitsu Iino

Article

Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

Insert

R-CEPIA4mt

Tags

Myc

Expression

Mammalian

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1 T2 CRIPR in pX330

Plasmid

#72833

Purpose

CRISPR/Cas to target the AAVS1 locus in human cells

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

Cas9 and gRNA for targeting the AAVS1 locus in human cells

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-STING_gRNA3

Plasmid

#127640

Purpose

Knock-out of human STING

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry

Plasmid

#176016

Purpose

Mammalian expression of mCherry. Parton lab clone DFQ

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

mCherry

Expression

Mammalian

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG2ABC-eIF3Δbgi

Plasmid

#240043

Purpose

Human eIF3k, eIF3l, eIF3e, eIF3f, eIF3h, eIF3d, eIF3m, eIF3a and eIF3c expression in insect cells for purification

Depositor

Venki Ramakrishnan

Article

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

eIF3k, l, e, f, h, d, m, a, c

Tags

eIF3a: TEV_3xFLAG, eIF3c: 3C_STREP II, eIF3d: HA

Expression

Insect

Mutation

WT

Promoter

Polyhedrin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-RIG-I

Plasmid

#240814

Purpose

Expresses myc-tagged RIG-I in mammalian cells

Depositor

AJ te Velthuis

Insert

RIG-I (RIGI Human)

Tags

Myc

Expression

Mammalian

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAF0033 Ef1a-Pa01-2a-EGFP

Plasmid

#193460

Purpose

Expression of Pa01 recombinase from Ef1a promoter

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-PEmax for IVT

Plasmid

#178113

Purpose

Template for in vitro transcription of PEmax

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Mutation

Detailed in manuscript

Promoter

T7 (inactivated)

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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GalT-RpHLuorin2

Plasmid

#171719

Purpose

Trans-Golgi expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to B4GALT1 (B4GALT1 Human)

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSIRV-NF-kB-eCFP

Plasmid

#118094

Purpose

Flourescent reporter for NF-kB activity

Depositor

Peter Steinberger

Article

Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

Insert

eCFP

Use

Retroviral

Promoter

minimal promoter with NF-kB responsive element

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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ptfLC3

Plasmid

#21074

Purpose

Mammalian expression of rat LC3 fused to mRFP and EGFP

Depositor

Tamotsu Yoshimori

Article

Kimura et al Autophagy. 2007;3(5):452-60.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

EGFP and mRFP

Expression

Mammalian

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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EGFP-alphasynuclein-A53T

Plasmid

#40823

Depositor

David Rubinsztein

Article

Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

Insert

SNCA (SNCA Human)

Tags

EGFP

Expression

Mammalian

Mutation

A53T (aggregates more rapidly than the wild-type …

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pKD3

Plasmid

#45604

Depositor

Barry L. Wanner

Article

Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(

Insert

FRT-cat-FRT

Expression

Bacterial

Promoter

R6K

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

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pRL623

Plasmid

#58494

Purpose

helper plasmid for bacterial conjugal DNA transfer

Depositor

Peter Wolk

Article

Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

Insert

encodes methylases AvaiM, Eco47iiM, and Ecot22iM that target sites subject to restriction in Anabaena sp. strain PCC 7120

Available Since

Oct. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pCCL/IFNB1-d2eGFP-3’UTR

Plasmid

#180232

Purpose

Reporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Front Cell Infect Microbiol. 2022 Jan 18

Insert

d2eGFP

Use

Lentiviral

Promoter

1 kb upstream of IFNB1 CDS

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-miniTurbo-NLS-Blast

Plasmid

#232347

Purpose

Lentiviral control vector expressing miniTurboNLS without dCas9

Depositor

Ali Shilatifard

Article

Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

Insert

miniTurbo

Use

Lentiviral

Tags

3xHA and 3xSV40 NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHCMV_DENV2_PrME

Plasmid

#207264

Purpose

Mammalian expression of Dengue virus 2 PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

DENV2_PrME (POLY Dengue virus 2)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 phiC31

Plasmid

#68310

Purpose

In vitro transcription vector to generate phiC31 mRNA (i.e. for zebrafish injection).

Depositor

Konrad Basler

Article

Bischof et al Proc Natl Acad Sci U S A. 2007 Feb 27;10

Insert

phiC31

Use

Rna transcription

Promoter

CMV

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pAG/MNase

Plasmid

#123461

Purpose

Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.

Depositor

Steven Henikoff

Article

Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1

Insert

Protein A-Protein G-Micrococcal Nuclease

Tags

6xHIS and HA

Expression

Bacterial

Promoter

Lac

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pTRE-Tight-MitoTimer

Plasmid

#50547

Purpose

Expresses the Timer protein targeted to the mitochondria under a Tet-inducible promoter

Depositor

Roberta Gottlieb

Article

Hernandez et al Autophagy. 2013 Nov 1;9(11):1852-61. doi

Insert

MitoTimer

Expression

Mammalian

Mutation

F12S mutation in Timer

Available Since

March 14, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-GFP1-10-IRES-Puro

Plasmid

#211467

Purpose

Lentivirus backbone expressing GFP1-10

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

GFP1-10

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMT525

Plasmid

#131392

Purpose

L1RP retrotransposition reporter

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

L1RP GFP-AI

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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Luciferase-pcDNA3

Plasmid

#18964

Depositor

William Kaelin

Article

Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

Insert

Firefly Luciferase

Expression

Mammalian

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pCE-mp53DD

Plasmid

#41856

Purpose

Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

Trp53 (Trp53 Mouse)

Expression

Mammalian

Mutation

Δ40-903

Promoter

CAG

Available Since

July 18, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2+MLS-HyPer7

Plasmid

#136470

Purpose

Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Tandem mitochondrial targeting signal of cytochro…

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-ER-3

Plasmid

#55041

Purpose

Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

ER (CALR Human)

Tags

mCherry

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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