159,238 results

1. mini-pHelper-1.0

   Plasmid

   #230933

   Purpose

   The mini verison (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV production

   Depositor

   Qizhao Wang

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3xHA-TurboID-NLS_pCDNA3

   Plasmid

   #107171

   Purpose

   expresses 3xHA-tagged TurboID in the mammalian nucleus

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hIBA1a-GFP-miR124T

   Plasmid

   #214147

   Purpose

   AAV vector to restrict GFP expression in microglia.

   Depositor

   Chun-Li Zhang

   Article

   Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiFUCCI(CA)5

   Plasmid

   #223176

   Purpose

   Lentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)

   Depositor

   Neville Sanjana

   Article

   Liang et al Cell. 2024 Dec 26;187(26):7637-7654.e29.

   Insert

   FUCCI(CA)5
   

   Use

   Lentiviral

   Tags

   AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJSK659

   Plasmid

   #205128

   Purpose

   Expression of GFP-SnxA in mammalian cells

   Depositor

   Jason King

   Article

   Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

   Insert

   SnxA
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-HA-Ubiquitin-WT

   Plasmid

   #17608

   Purpose

   Mammalian expression of HA tagged ubiquitin

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV SCH9

   Plasmid

   #212708

   Purpose

   A SCHEMA evolved AAV variant effective at targeting subventricular NSCs

   Depositor

   David Schaffer

   Article

   Ojala et al Mol Ther. 2018 Jan 3;26(1):304-319. doi:

   Insert

   AAV2 Rep SCH9 Cap
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP1653 - pAAV-AiE2333m-minBG-SYFP2-WPRE-BGHpA

   Plasmid

   #214506

   Purpose

   AiE2333m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   The Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRE-Tight-MitoTimer

   Plasmid

   #50547

   Purpose

   Expresses the Timer protein targeted to the mitochondria under a Tet-inducible promoter

   Depositor

   Roberta Gottlieb

   Article

   Hernandez et al Autophagy. 2013 Nov 1;9(11):1852-61. doi

   Insert

   MitoTimer
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F12S mutation in Timer

   Promoter

   Available since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-myc-FLNa WT

    Plasmid

    #8982

    Depositor

    John Blenis

    Article

    Woo et al Mol Cell Biol. 2004 Apr . 24(7):3025-35.

    Insert

    Filamin A (FLNA Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-10His-mStayGold (E138D)

    Plasmid

    #211363

    Purpose

    Mammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    mStayGold
    

    Use

    Tags

    10xHis-tag

    Expression

    Mammalian

    Mutation

    E138D

    Promoter

    CMV

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)

    Plasmid

    #199604

    Purpose

    Expression of cell permeable spCas9 in E. Coli.

    Depositor

    Junwei Shi

    Article

    Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

    Insert

    Cas9-T6N
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPSU250

    Plasmid

    #173837

    Purpose

    Expresses 250 kD Penn State ladder protein

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPABPACIL1bQRSSTRHSTPABMBPpepN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. mARG1 cassette 1

    Plasmid

    #134343

    Purpose

    Mammalian acoustic reporter gene 1 cassette 1 on the piggyBac trasposon plasmid.

    Depositor

    Mikhail Shapiro

    Article

    Farhadi et al Science. 2019 Sep 27;365(6460):1469-1475

    Insert

    mARG 1 cassete 1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Nov. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. mARG1 cassette 2

    Plasmid

    #134344

    Purpose

    Mammalian acoustic reporter gene 1 cassette 2 on the piggyBac trasposon plasmid.

    Depositor

    Mikhail Shapiro

    Article

    Farhadi et al Science. 2019 Sep 27;365(6460):1469-1475

    Insert

    mARG 1 cassete 2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Nov. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-YFP

    Plasmid

    #11180

    Purpose

    Mammalian expression vector for expression of YFP

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Insert

    EYFP from Aequorea victoria
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAVS1-Pur-CAG-EGFP

    Plasmid

    #80945

    Purpose

    human AAVS1 site targeting donor plasmid for knocking-in EGFP expression cassette driven by CAG promoter

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMitoTimer

    Plasmid

    #52659

    Purpose

    Expresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivo

    Depositor

    Zhen Yan

    Article

    Laker et al J Biol Chem. 2014 Apr 25;289(17):12005-1

    Insert

    DsRed1-E5
    

    Use

    Dsred

    Tags

    human cytochrome c oxidase subunit VIII mitochond…

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AiP12385 - pAAV-AiE0548h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2385)

    Plasmid

    #223990

    Purpose

    AiE0548h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L4/5_IT neurons

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    minBG

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-EF1a-Cas9Bsd-W

    Plasmid

    #68343

    Purpose

    Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-Cas9 cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Cas9 fused with Flag-tag and a NLS at the N termi…

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGSKU

    Plasmid

    #72243

    Purpose

    Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

    Depositor

    Michael Resnick, Francesca Storici

    Article

    Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

    Inserts

    KlURA3
    kanMX4
    I-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFGET19_Ulp1

    Plasmid

    #64697

    Purpose

    H6-Ulp1 from pHYRS52 into pET-28b(+)

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Insert

    ULP1 (ULP1 Budding Yeast, Saccharomyces cerevisiae)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Ulp1(403-621)

    Promoter

    T7

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3/F-tractin=mStayGold

    Plasmid

    #212019

    Purpose

    For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    F-tractin
    

    Use

    Tags

    mStayGold

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-hSyn-FLEX-RiboL1-jGCaMP8s

    Plasmid

    #192616

    Purpose

    AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8s.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    FLEX-RIboL1-jGCaMP8s
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCS2+IMS-HyPer7

    Plasmid

    #136469

    Purpose

    Mammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    SMAC/DIABLO

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. Lamp1_mStayGold

    Plasmid

    #221902

    Purpose

    Expresses mStayGold fused to Lamp1

    Depositor

    Janelia Integrative Imaging

    Insert

    Lamp1_mStayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. L4440

    Plasmid

    #1654

    Depositor

    Andrew Fire

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    RNAi

    Tags

    T7p, T7p, lacZN, OriF1>>, OriF1<<

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Lenti-Cas9-gRNA-GFP

    Plasmid

    #124770

    Purpose

    3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMH-Halo tag

    Plasmid

    #154144

    Purpose

    Gateway compatible mammalian expression plasmid for halo tag fusion proteins

    Depositor

    Michael Huen

    Type

    Empty backbone

    Use

    Tags

    Halo tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. dCAS9-VP64_GFP

    Plasmid

    #61422

    Purpose

    Expresses dCAS9-VP64 activator with 2A GFP

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Concentrated Lentiviral Prep

    Insert

    dCAS9(D10A,H840A)-VP64_2A_GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    EF1A

    Available since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge