We narrowed to 3,429 results for: Agt

1. Lenti-SLC35B2-sgRNA

   Plasmid

   #154860

   Purpose

   Lentiviral expression of Cas9 and sgRNA targeting SLC35B2

   Depositor

   Yihong Ye

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

   Insert

   SLC35B2 sgRNA (SLC35B2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV10.K5

   Plasmid

   #63210

   Purpose

   pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAV10.K4

   Plasmid

   #63212

   Purpose

   pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSMP-Bmi1_2

   Plasmid

   #36353

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   Bmi1 (BMI1 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pAV11K

   Plasmid

   #63184

   Purpose

   pRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pORANGE WASF1-GFP KI

   Plasmid

   #131502

   Purpose

   Endogenous tagging of WASP1/WAVE1: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Wasf1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. sgLacZs

   Plasmid

   #171185

   Purpose

   Expresses sgRNA

   Depositor

   Han Kyoung Choe

   Article

   Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

   Insert

   hU6-sgLacZ1-hU6-sgLacZ2
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   U6, hSyn

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX458_FOXP1_2

   Plasmid

   #86300

   Purpose

   Encodes gRNA for 3' target of human FOXP1

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against FOXP1 (FOXP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMKO.1 puro-luc (shRNA)

   Plasmid

   #153962

   Purpose

   Expresses a shRNA against firefly luciferase

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   shRNA against firefly luciferase (19-bp-long target)
   

   Use

   Luciferase, RNAi, and Retroviral

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX458-sgRNA_Ago1_1

    Plasmid

    #73533

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV Gsk3 sgRNA/GFP

    Plasmid

    #112733

    Purpose

    Gsk3b targeting gRNA cloned into px552 (SpGuide) plasmid.

    Depositor

    Martin Beaulieu

    Article

    Khlghatyan et al Front Mol Neurosci. 2018 Apr 12;11:119.

    Insert

    GFP
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pS23.U6<ActB.mus_C-term-KI_gRNA

    Plasmid

    #207408

    Purpose

    gRNA from a U6 promoter targeting the mouse ActB near the 3' end of the ORF. Used for C-terminal knockin by DSB repair. [Lab plasmid ID: TU260]

    Depositor

    Alexandros Poulopoulos

    Article

    Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

    Insert

    ActB
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. FOXM1 C11.2 gRNA

    Plasmid

    #90690

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FOXM1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    FOXM1 (Guide Designation C11.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry

    Plasmid

    #84260

    Purpose

    Expresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulation

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    Sp sgSV40
    PYL1-KRAB
    

    Use

    CRISPR and Lentiviral

    Tags

    IRES-mCherry and PYL1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and mouse U6

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. POT1 E3.4 gRNA

    Plasmid

    #90842

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human POT1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    POT1 (Guide Designation E3.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LentiCRISPR v2-sgAMPK alpha1 clone2

    Plasmid

    #162120

    Purpose

    Lentiviral sgRNA plasmid targeting human AMPK alpha1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgAMPK alpha1 (PRKAA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGMC00013

    Plasmid

    #172525

    Purpose

    sgRNA against mouse Mr1

    Depositor

    Raj Chari

    Article

    Ruf et al Cancer Immunol Res. 2021 Jun 30. pii: 23

    Insert

    Mr1 (Mr1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXPR_sgGFP4

    Plasmid

    #119876

    Purpose

    gRNA expression vector

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    sgGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSirenRetroQ(puro)-shEgr4

    Plasmid

    #108117

    Purpose

    pSIREN-RetroQ vector containing shRNA sequence to Egr4

    Depositor

    Peter Johnson

    Article

    Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

    Insert

    shRNA to Egr4 (Egr4 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSMP-EED_2

    Plasmid

    #36386

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    EED (EED Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CEP55 B12.2 gRNA

    Plasmid

    #90623

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CEP55

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CEP55 (Guide Designation B12.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Nov. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CEP55 B11.2 gRNA

    Plasmid

    #90622

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CEP55

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CEP55 (Guide Designation B11.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. tet pLKO.1-shCSE1L v1 puro

    Plasmid

    #192345

    Purpose

    Lentiviral expression vector for an inducible shCSE1L v1

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    shCSE1L v1 (CSE1L Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLKO.1-Tet-Puro-shMMERVK10c_1 (inducible)

    Plasmid

    #189955

    Purpose

    shRNA mediated knockdown

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    ERV_MMERVK10c
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FOXM1 C12.2 gRNA

    Plasmid

    #90691

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FOXM1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    FOXM1 (Guide Designation C12.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSMP-SETDB1_2

    Plasmid

    #36348

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    SETDB1 (SETDB1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

27. Tol2-LyzC-RacFRET-U6ac-rac2 guides

    Plasmid

    #168248

    Purpose

    "label active Rac under Rac2 neutrophil-specific knockout background"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    RacFRET
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    LyzC

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti-Hygro-sgTom

    Plasmid

    #138698

    Purpose

    Expresses a tomato-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ANAPC1 A1.3 gRNA

    Plasmid

    #90528

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ANAPC1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    ANAPC1 (Guide Designation A1.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. shCTL-mlpx-puro

    Plasmid

    #65232

    Purpose

    encodes a non-targeting control shRNA

    Depositor

    Gerardo Ferbeyre

    Article

    Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

    Insert

    non-coding shRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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