We narrowed to 9,389 results for: Coli

1. G555

   Plasmid

   #216483

   Purpose

   Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.

   Depositor

   Christophe Danelon

   Insert

   plsB, plsC, cdsA, pssA
   

   Use

   Cell-free expression

   Tags

   Expression

   Mutation

   Promoter

   T7 for each gene

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAN1717

   Plasmid

   #74696

   Purpose

   RPU standard plasmid

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

   Insert

   Insulated J23101-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSB1A3-AD011

   Plasmid

   #116852

   Purpose

   Expresses araC constitutively and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD011

   Depositor

   Friedrich Simmel

   Article

   Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

   Inserts

   AraC
   mScarletI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTW334e

   Plasmid

   #237315

   Purpose

   expresses A. thaliana Cpn60α Cpn60β Cpn20 Raf1 Raf2 RbcX Bsd2

   Depositor

   Tina Wang

   Article

   Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

   Insert

   A. thaliana Cpn60α Cpn60β Cpn20 Raf1 Raf2 RbcX Bsd2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKB232

   Plasmid

   #170019

   Purpose

   Encodes LacI-controlled expression of surface-displayed murine CRAMP and PvirK-mNeonGreen reporter

   Depositor

   Jeffrey Tabor

   Article

   Brink et al bioRxiv

   Inserts

   mNeonGreen
   CRAMP
   

   Use

   Synthetic Biology

   Tags

   Lpp, OmpA, and Tether

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac and PvirK

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBbS5c-mCherry-AsLOV2*(543)

   Plasmid

   #202712

   Purpose

   Expresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)

   Depositor

   Mary Dunlop

   Article

   Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

   Insert

   mCherry-AsLOV2*(543)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBEST-OR2-OR1-Pr-UTR1-deGFP-T500

   Plasmid

   #40019

   Depositor

   Vincent Noireaux

   Article

   Shin et al J Biol Eng. 2010 Jun 24;4:8.

   Insert

   deGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

   Available since

   Oct. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21-Streptavidin-Glutamate_Tag

   Plasmid

   #46367

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

   Insert

   Streptavidin-Glutamate_Tag
   

   Use

   Tags

   6 glutamate tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pAH69

   Plasmid

   #173782

   Purpose

   The pAH69 plasmid [Haldimann and Wanner 2001] is the HK022 helper plasmid necessary for genomic integration of the pIT5_KH plasmid.

   Depositor

   Keith Shearwin

   Article

   Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

   Insert

   HK022 Integrase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-R1R2-EM7neoPheS

    Plasmid

    #112923

    Purpose

    piggyBac Transposon vector with a Gateway destination cassette (neoPheS selection)

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    neoPheS
    

    Use

    Piggybac transposon gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKL09-GCaMP6f-mScarlet bb118

    Plasmid

    #158979

    Purpose

    Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    GCaMP6f-mScarlet fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#100833

    Promoter

    Bba_J23118 (sigma 70)

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC28-LIC-sfGFP-His

    Plasmid

    #165479

    Purpose

    pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

    Depositor

    Renato Bruni

    Article

    Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

    Type

    Empty backbone

    Use

    Tags

    TEV-GFP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRF-BetI

    Plasmid

    #49357

    Depositor

    Christopher Voigt

    Article

    Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

    Insert

    BetI repressor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMA2641

    Plasmid

    #25435

    Depositor

    Mikhail Alexeyev

    Article

    Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

    Insert

    reverse tetracycline-regulated transactivator advanced
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAT112TAG-SepT

    Plasmid

    #34624

    Depositor

    Jesse Rinehart, Dieter Söll

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Insert

    tRNA^Sep
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTW357bN

    Plasmid

    #227932

    Purpose

    Accessory plasmid for negative selection

    Depositor

    Tina Wang

    Article

    Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

    Inserts

    gIII-neg
    T7c
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R632S

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTW357d5

    Plasmid

    #227931

    Purpose

    High-stringency accessory plasmid for selection

    Depositor

    Tina Wang

    Article

    Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

    Inserts

    gIII (recoded)
    T7c
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R632S, Q649S

    Promoter

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKB231

    Plasmid

    #170018

    Purpose

    Encodes LacI-controlled expression of surface-displayed cecropin P1 and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    Cecropin P1
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMYO

    Plasmid

    #34626

    Depositor

    Jesse Rinehart, Dieter Söll

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Insert

    Myoglobin
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    codon-optimized

    Promoter

    lpp

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pADS

    Plasmid

    #19040

    Depositor

    Jay Keasling

    Article

    Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

    Insert

    codon-optimized synthetic amorphadiene synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

21. pBSKrnaSeph

    Plasmid

    #67506

    Purpose

    Express the tRNALys scaffold with the sephadex aptamer tag

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

    Insert

    modified tRNALys with sephadex aptamer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pAdEasy 2-GFP beta-gal

    Plasmid

    #16407

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

23. pNO183

    Plasmid

    #101068

    Purpose

    ppsR2-bphP1 bicistronic operon plasmid

    Depositor

    Jeffrey Tabor

    Article

    Ong et al ACS Synth Biol. 2017 Nov 1. doi: 10.1021

    Inserts

    ppsR2 (ppsR2 Rhodopseudomonas palustris CGA009)
    bphP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    insert guanine at position 430 to fix frameshift …

    Promoter

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMA3735

    Plasmid

    #184848

    Purpose

    gRNA cloning using blue-white screening for recombinants

    Depositor

    Mikhail Alexeyev

    Article

    Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

    Insert

    LacZalpha
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    alpha-fragment of LacZ

    Promoter

    U6

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p007ampGFP

    Plasmid

    #58535

    Purpose

    GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; ampicillin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    Green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5 between two lac operators

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p44K

    Plasmid

    #45800

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGC4593

    Plasmid

    #110206

    Purpose

    Accessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)

    Depositor

    Guillaume Cambray, Adam Arkin

    Article

    Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

    Inserts

    TEV protease
    aaRS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pbad and Ptet

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pNM3814

    Plasmid

    #154122

    Purpose

    mec-4 promoter driving tet ON rtetR-L-QFAD in an RCME vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    mec-4p::rtetR-L-QFAD::act-4 '3
    

    Use

    Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTK222

    Plasmid

    #13532

    Depositor

    Wolf Frommer

    Article

    Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

    Insert

    FLIPW-CTYT
    

    Use

    Tags

    See map

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSWAP_Entry_H2_beta_CM

    Plasmid

    #184870

    Purpose

    pSwap plasmid part with H2_beta homology arms and chloramphenicol cassette

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 2, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pET29b-clbO

    Plasmid

    #114153

    Purpose

    Expresses ClbO

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbO
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. SJZ7-KAN

    Plasmid

    #228260

    Purpose

    Template plasmid for yeast gene knockout, confers antibiotic resistance to G418

    Depositor

    Zhiping Xie

    Article

    Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

    Insert

    kan
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPM1 S.c.

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pICSL11137

    Plasmid

    #191783

    Purpose

    Kanamycin resistance in plants

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    35S::nptII-T-Nos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMARO

    Plasmid

    #178086

    Purpose

    Derivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.

    Depositor

    Sören Abel

    Article

    Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10

    Insert

    TcR (tetA/tetR)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Tetracycline resistance cassette is flanked by Ec…

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28a-clbK

    Plasmid

    #114152

    Purpose

    Expresses ClbK

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbK
    

    Use

    Tags

    6xhis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET29b-clbI

    Plasmid

    #114151

    Purpose

    Expresses ClbI

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbI
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET29b-clbF

    Plasmid

    #114148

    Purpose

    Expresses ClbF-cHis

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    ClbF
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28a-clbC

    Plasmid

    #114145

    Purpose

    Expresses ClbC-nHis

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbC
    

    Use

    Tags

    6xHis-thrombin-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET29b-clbE

    Plasmid

    #114147

    Purpose

    Expresses ClbE-cHis

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    ClbE
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET29b-clbD

    Plasmid

    #114146

    Purpose

    Expresses ClbD-cHis

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    ClbD
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge