Addgene

We narrowed to 16,058 results for: JUN

Showing: 3151 - 3200 of 16058 results

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CMV-Halo-10-PFN1-G118V

Plasmid

#204225

Purpose

Expression of Halo-Profilin-1(G118V) in tissue culture cells

Depositor

Jessica Henty-Ridilla

Article

Pimm et al Elife. 2022 Jun 6;11:e76485. doi: 10.755

Insert

PFN1 (PFN1 Human)

Use

Tags

Halo, TEV site, SGLRSRAQAS linker

Expression

Mammalian

Mutation

G118V

Promoter

CMV

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-mTagBFP-T2A-TetOn3G

Plasmid

#179888

Purpose

Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

TetOn3G

Use

Lentiviral

Tags

mTagBFP

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1a

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pMSSM151: pU6-NlovFz2_omegaRNA-entry

Plasmid

#205263

Purpose

Human expression vector for NlovFz2 omegaRNA (BsmBI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2 omegaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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dGFP-Rab3a

Plasmid

#170864

Purpose

Mammalian expression of dGFP-Rab3a.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rab-3a (Rab3a Mouse)

Use

Tags

dEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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CSII-TK-MCS

Plasmid

#203597

Purpose

Empty lentiviral vector to express protein in mammalian cells cells.

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TK

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAW2-Smed-Slit-1

Plasmid

#182264

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Slit-1

Depositor

Daniel Lobo

Article

Wolff et al STAR Protoc. 2022 Jun 14;3(3):101458. do

Insert

Smed-Slit-1

Use

Gibson cloning vector for synthesis of single and…

Tags

Expression

Mutation

Promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMJ240: pRS426-His-MBP-MmeFz2_Native

Plasmid

#205255

Purpose

S. cerevisiae expression vector for MmeFz2 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

MmeFz2

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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HA-mULK1M92A

Plasmid

#31964

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK1 (Ulk1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

M92A

Promoter

CMV, SP6

Available since

Oct. 31, 2011

Availability

Academic Institutions and Nonprofits only
Lapronic/pRSETB

Plasmid

#140755

Purpose

FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

Depositor

Luis Felipe Barros

Article

Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

Insert

Lapronic (Lactate/PYRuvate Ratio Optical Nano-Indicator from CECs)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pRha-tns

Plasmid

#170630

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

VchTnsABC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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piCRg Entry

Plasmid

#58904

Purpose

Cas9/gRNA Entry expression plasmid

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis-MMLV RT

Plasmid

#178797

Purpose

Inducible expression of MMLV RT in E. coli

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

MMLV RT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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CpxR-GFP

Plasmid

#220150

Purpose

To track the natural CpxR promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

CpxR promoter only

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SiT-EGFP

Plasmid

#166943

Purpose

mammalian expression of N' Sialyl Transferase tagged with EGFP

Depositor

Jennifer Lippincott-Schwartz

Article

Patterson et al Cell. 2008 Jun 13;133(6):1055-67. doi: 1

Insert

Sialyl Transferase N-terminus

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-neo-hCG

Plasmid

#16574

Depositor

Bert Vogelstein

Article

Shih et al Nat Med. 2000 Jun . 6(6):711-4.

Insert

Beta-hCG (CGB3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
k3(long) luciferase

Plasmid

#11110

Depositor

Anjana Rao

Article

Tsai et al Mol Cell Biol. 1996 Feb . 16(2):459-67.

Insert

k3(L) (TNF Human)

Use

Tags

luciferase

Expression

Bacterial

Mutation

Promoter

Available since

April 24, 2006

Availability

Academic Institutions and Nonprofits only
pBluescript-Evf1

Plasmid

#99479

Purpose

Subcloning of the Evf1 long non-coding RNA

Depositor

Jhumku Kohtz

Article

Feng et al Genes Dev. 2006 Jun 1;20(11):1470-84. Ep

Insert

Evf1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmMe31B

Plasmid

#21682

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

maternal expression at 31B (me31B Fly)

Use

Drosophila expression

Tags

EGFP

Expression

Mutation

Promoter

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Lysosomes-20

Plasmid

#58022

Purpose

Localization: Lysosome Membrane, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

LAMP1

Use

Tags

mTagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCS2-SP-FRB (T2098L) -sfGFP-TM-V5

Plasmid

#186227

Purpose

mRNA synthesis of rapalog-induced cell adhesion molecule

Depositor

Yoshihiro Morishita

Article

Usami et al Genes Cells. 2022 Jun;27(6):436-450. doi

Insert

mRNA synthesis of rapalog-induced cell adhesion molecule

Use

Tags

V5

Expression

Mammalian

Mutation

FRB (T2098L)

Promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-myc-SNAP-GP135

Plasmid

#50376

Purpose

Express myc and SNAP-tagged GP135 in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

SNAP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pET28b::mAID

Plasmid

#15923

Depositor

Nina Papavasiliou

Article

Besmer et al Mol Cell Biol. 2006 Jun . 26(11):4378-85

Insert

AID (Aicda Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pARR5::LUC

Plasmid

#141170

Purpose

Luminescent reporter for cytokinin signaling

Depositor

Vardis Ntoukakis, Patrick Schäfer

Article

Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

Insert

AtARR5 promoter

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

AtARR5, AT3G48100

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST51-LRRK2-R1441C

Plasmid

#25081

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Destination vector

Tags

6XHis and V5

Expression

Mammalian

Mutation

Arginine 1441 mutated to Cysteine

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pRE57I-GNAc

Plasmid

#170637

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

Mini-Tn6677, GNAc cassette, lacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p1.1

Plasmid

#162737

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x7

Plasmid

#58777

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
miniTn7(Gm)PA1/04/03-eyfp-a

Plasmid

#111620

Purpose

eYFP Fluorescent tagging of bacteria at their Tn7-site

Depositor

Tim Tolker-Nielsen

Article

Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

Insert

PA1/04/03-eyfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRetroSuper-GFP Smad3

Plasmid

#15723

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad3 shRNA (SMAD3 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pCB6-HFE-EGFP

Plasmid

#12104

Depositor

Pamela Bjorkman

Article

Giannetti et al J Biol Chem. 2004 Jun 11. 279(24):25866-

Insert

HFE (HFE Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pMJ235: pRS426-His-MBP-GtFz1_Native

Plasmid

#205254

Purpose

S. cerevisiae expression vector for GtFz1 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-HArcLight

Plasmid

#138326

Purpose

chemigenetic voltage indicator

Depositor

Eric Schreiter

Article

Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

Insert

HArcLight

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJFRC7-UAS-NPRR_ANP

Plasmid

#133243

Purpose

Fluorescent reporter for neuropeptide release imaging

Depositor

David Anderson

Article

Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

Insert

rat atrial natriuretic peptide-fused codon-optimized GCaMP6s

Use

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRAB18::LUC

Plasmid

#141168

Purpose

Luminescent reporter for abscisic acid signaling

Depositor

Vardis Ntoukakis, Patrick Schäfer

Article

Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

Insert

AtRAB18 promoter

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

AtRAB18, AT5G66400

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5/FRT-ChemoG1

Plasmid

#193806

Purpose

CMV overexpression of ChemoG1 in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG1

Use

Tags

10xHis

Expression

Mammalian

Mutation

EGFP: A206K

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Bmp5

Plasmid

#163589

Purpose

Expresses BMP-5 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp5 (Bmp5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT85_PS-mEos3.2_GentaR

Plasmid

#173895

Purpose

Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the Spiralin promoter

Depositor

Yonathan Arfi

Article

Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

Insert

mEos3.2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Spiralin

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-LAP2-YFP-NLS

Plasmid

#25847

Depositor

Alice Ting

Article

Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

Insert

LAP2-YFP-NLS

Use

Tags

His and NLS

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pPM2212

Plasmid

#167552

Purpose

Encodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12

Depositor

Renato Morona

Article

Macpherson et al Mol Microbiol. 1991 Jun;5(6):1491-9. doi

Insert

rfb region of Shigella flexneri serotype 2a

Use

Cosmid

Tags

Expression

Mutation

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag pLJM1 RagD 77L

Plasmid

#19317

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

S77L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pLPCX roGFP2-Prx2

Plasmid

#169230

Purpose

Genetically encoded fluorescent H2O2 sensor roGFP2-Prx2 for cytosolic expression in mammalian cells (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

roGFP2-Prx2

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad33_ISTfu1_reRNA

Plasmid

#205355

Purpose

Bacterial expression of ISTfu1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISTfu1 TnpB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDrop

Plasmid

#184872

Purpose

Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONRPEX18Km-SceI-pheS

Plasmid

#111610

Purpose

Gateway compatible allelic exchange vector for Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Type

Empty backbone

Use

Allelic exchange in burkholderia sp.

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 RagB shRNA #2

Plasmid

#26628

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagB shRNA (RRAGB Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pAG499-FRB-N-frFAST

Plasmid

#160059

Purpose

Expresses FRB-N-frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

FRB-N-frFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPCX roGFP2-Prx2(C172A)

Plasmid

#158228

Purpose

Genetically encoded fluorescent H2O2 sensor roGFP2-Prx2(C172A) for cytosolic expression in mammalian cells (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

roGFP2-Prx2(C172A)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEAK10-His-Myc-Kal12

Plasmid

#25442

Depositor

Betty Eipper

Article

Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

Insert

Kal12

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
pET-51b(+) ChemoG5

Plasmid

#193805

Purpose

Protein production of ChemoG5 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG5

Use

Tags

10xHis

Expression

Bacterial

Mutation

EGFP:A206K, T225R; HT7: E143R, E147R, L271E

Promoter

T7

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SpyCas9(WT)

Plasmid

#101044

Purpose

Express Streptococcus pyogenes Cas9

Depositor

Zhiwei Huang

Article

Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

Insert

SpyCas9

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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