We narrowed to 12,292 results for: BASE

1. pVKG1-Puro

   Plasmid

   #108670

   Purpose

   Puromycin resistant cassette for knock-in by VIKING method

   Depositor

   Shigeo Sugano

   Article

   Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

   Insert

   PURO
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA-D3cpv

   Plasmid

   #36323

   Depositor

   Roger Tsien, Amy Palmer

   Article

   Palmer et al Chem Biol. 2006 May;13(5):521-30.

   Insert

   D3cpv second generation cameleon (calcium sensor)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SpCas9-CBE6d-IVT

   Plasmid

   #215836

   Purpose

   Template for in vitro transcription of CBE6d (with SpCas9)

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6d-SpCas9-2xUGI
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCRISPaint-2A-TurboGFP-PEST

   Plasmid

   #67180

   Purpose

   CRISPaint universal donor for gene expression quantification using destabilized TurboGFP

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   T2A-TurboGFP-PEST
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSEVA221TadA*-T7RNAP

   Plasmid

   #167980

   Purpose

   Expresses fusion protein TadA*-T7RNAP in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

   Insert

   TadA*-T7RNAP
   

   Use

   Tags

   T7 tag and T7RNAP

   Expression

   Bacterial

   Mutation

   Promoter

   TetR-Ptet

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p663-UBC-V5-GSK3B_IDG-K

   Plasmid

   #158166

   Purpose

   Gateway destination clone of V5-GSK3B (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   V5-GSK3B (GSK3B Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC18-mini-Tn7T-Gm-GW

   Plasmid

   #64961

   Purpose

   mini-Tn7 Gateway destination vector

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pET24b-SUMO-BG2.4

   Plasmid

   #199438

   Purpose

   expresses BAmGreen2.4 in bacterial cells with a SUMO tag

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   BAMGreen2.4
   

   Use

   Tags

   his tag and sumo

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p663-UBC-miniTurbo-V5-MAPK15_IDG-K

   Plasmid

   #170581

   Purpose

   Gateway destination clone of MAPK15 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-MAPK15 (MAPK15 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHR iSH-YFP-PIF

    Plasmid

    #50841

    Purpose

    Optogenetic PI3K activation system; cytoplasmic component

    Depositor

    Wendell Lim

    Article

    Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

    Inserts

    p85 iSH2
    PIF6 1-100
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET29b_1_anti-GFP-nanobody

    Plasmid

    #210394

    Purpose

    Express anti-GFP nanobody in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    1_anti-GFP-nanobody
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pENTR5'_ubi:loxP-EGFP-loxP

    Plasmid

    #27322

    Depositor

    Leonard Zon

    Article

    Mosimann et al Development. 2011 Jan . 138(1):169-77.

    Insert

    ubiquitin:loxP-EGFP-loxP
    

    Use

    Multisite gateway 5' entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LRT2B-AdGO2

    Plasmid

    #136911

    Purpose

    Lentivirus expressing AdGO2 sgRNA

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    sgAdGO2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    hU6

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p667-UBC-BCKDK-V5-miniTurbo_IDG-K

    Plasmid

    #158189

    Purpose

    Gateway destination clone of BCKDK (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    BCKDK-V5-miniTurbo (BCKDK Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(+)-OTI-TCRA_p2_TCRB

    Plasmid

    #131033

    Purpose

    Expresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptide

    Depositor

    Jeff Hammerbacher

    Article

    Aksoy et al bioRxiv

    Inserts

    TCRA (Tcra Mouse)
    TCRB (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pESC-TRP (V5 and HA)

    Plasmid

    #222488

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and TRP1 selection

    Depositor

    Kiyofumi Yamada, Tetsuo Matsuzaki

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSALECT-EcoBam

    Plasmid

    #59705

    Purpose

    Folding reporter with ampicillin resistance as a readout

    Depositor

    Christopher Anderson

    Article

    Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMSCVpuro-TagRFP657

    Plasmid

    #96939

    Purpose

    Retroviral plasmid containing TagRFP657 for labeling of cells

    Depositor

    Lee Grimes

    Article

    Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

    Insert

    Tag Red Fluorescent Protein 657
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETZZtevPP7CP(C68A,C73A)

    Plasmid

    #27548

    Depositor

    Kathleen Collins

    Article

    Hogg et al RNA. 2007 Jun . 13(6):868-80.

    Insert

    Pseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)
    

    Use

    Tags

    TEV protease cleavage site, six histidine tag, an…

    Expression

    Bacterial

    Mutation

    changed two cysteine to alanine (C68A,C73A) to in…

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p667-UBC-CDC42BPA-V5-miniTurbo_IDG-K

    Plasmid

    #189907

    Purpose

    Gateway destination clone of CDC42BPA (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    CDC42BPA (CDC42BPA Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLVX-EF1a-EGFP-RAB3C-IRES-Puromycin

    Plasmid

    #134857

    Purpose

    Expresses EGFP-tagged synaptic vesicle marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Ras-related protein 3C
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pESC-HIS (V5 and HA)

    Plasmid

    #222487

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and HIS3 selection

    Depositor

    Kiyofumi Yamada, Tetsuo Matsuzaki

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRibo EcoHind

    Plasmid

    #36252

    Depositor

    Carolyn Bertozzi, Jessica Seeliger

    Article

    Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

    Type

    Empty backbone

    Use

    Synthetic Biology; Mycobacteria replicating vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Modified Hsp60

    Available since

    April 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pD441_WT_hi

    Plasmid

    #128857

    Purpose

    High copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCRISPR-Cas9-ScaligD

    Plasmid

    #125688

    Purpose

    Gene knockout/in for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

    Insert

    streptomyces codon optimized spCas9, sgRNA casette, and ScaligD
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    ermE*/tipA

    Available since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEX128·gRNA

    Plasmid

    #187600

    Purpose

    Template for amplification of gRNA with Cas6 recognition site

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

    Insert

    gRNA template
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    no promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pVT36b

    Plasmid

    #184437

    Purpose

    CRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1’-tRNA_Leu promoter, and sgRNA scaffold

    Depositor

    Huimin Zhao

    Article

    Tran et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. T777T

    Plasmid

    #113082

    Purpose

    Enhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSEVA13-GG

    Plasmid

    #208002

    Purpose

    for cloning homology arms, with GFP as counter selection marker

    Depositor

    Pablo Ivan Nikel

    Article

    Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

    Insert

    msfGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    14G

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYZ1389

    Plasmid

    #215348

    Purpose

    Nla CRISPRi Delivery Vector

    Depositor

    Hank Seifert

    Article

    Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

    Insert

    Nla CRISPRi Genes (Cas5, Cas8c, Cas7)
    

    Use

    CRISPR; Nla i delivery vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLTR-RVG

    Plasmid

    #17577

    Depositor

    Jakob Reiser

    Article

    Mochizuki et al J Virol. 1998 Nov . 72(11):8873-83.

    Insert

    rabies virus glycoprotein (SAD B19 strain)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMU-ACTLr

    Plasmid

    #61193

    Purpose

    Fluorescent reporter for F-actin LifeAct-mCherry expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    LifeAct-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pESC-LEU (V5 and HA)

    Plasmid

    #222486

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and LEU2 selection

    Depositor

    Kiyofumi Yamada, Tetsuo Matsuzaki

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CellTagB

    Plasmid

    #124592

    Purpose

    Expressed barcode for sample multiplexing in single-cell analysis

    Depositor

    Samantha Morris

    Article

    Guo et al Genome Biol. 2019 May 9;20(1):90. doi: 1

    Insert

    CellTagB
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. SpCas9-CBE6a-IVT

    Plasmid

    #215833

    Purpose

    Template for in vitro transcription of CBE6a (with SpCas9)

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6a-SpCas9-2xUGI
    

    Use

    In vitro transcription template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMU-MTUBr

    Plasmid

    #61196

    Purpose

    Fluorescent reporter for microtubules mCherry-MAP4-MBD expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MAP-MBD
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFREE_Amp

    Plasmid

    #92051

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    pRhamBAD and ptet

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-PalmPalm-ClopHensor

    Plasmid

    #25940

    Depositor

    Daniele Arosio

    Article

    Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

    Insert

    PalmPalm-ClopHensor
    

    Use

    Tags

    Gap20 and Strep-II

    Expression

    Mammalian

    Mutation

    N-term (20AA) of Gap-43

    Promoter

    Available since

    Sept. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. ZipGFP11_TEV

    Plasmid

    #81243

    Purpose

    Zipped GFP11 with TEV cleavage sequence

    Depositor

    Xiaokun Shu

    Article

    To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

    Insert

    zipped GFP11 with TEV cleavage sequence
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. FlagMBNL3

    Plasmid

    #96901

    Purpose

    expression of Flag-tagged human MBNL3

    Depositor

    Thomas Cooper

    Article

    Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

    Insert

    human MBNL3 cDNA (MBNL3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge