Addgene

We narrowed to 16,058 results for: JUN

Showing: 3201 - 3250 of 16058 results

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2XMyc-LRRK2-RCKW

Plasmid

#25064

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Tags

2XMyc

Expression

Mammalian

Mutation

Contains only the ROC-COR-Kinase-WD40 domains …

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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pIAA5::LUC

Plasmid

#141169

Purpose

Luminescent reporter for auxin signaling

Depositor

Patrick Schäfer, Vardis Ntoukakis

Article

Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

Insert

AtIAA5 promoter

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

AtIAA5, At1g15580

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-deltaR8.2(A14C,E45C)

Plasmid

#79047

Purpose

mammalian expression, lentiviral packaging

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp10

Plasmid

#163593

Purpose

Expresses BMP-10 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp10 (Bmp10 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_LAMP1_STf

Plasmid

#182006

Purpose

CMV overexpression of SNAP-tag fast in the lysosome of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

LAMP1_STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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dGFP-Rab27a

Plasmid

#170862

Purpose

Mammalian expression of dGFP-Rab27a.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rab-27a (RAB27A Human)

Use

Tags

dEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoR-ATP

Plasmid

#193820

Purpose

CMV overexpression of ChemR-ATP in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoR-ATP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
2XMyc-LRRK2-Kinase

Plasmid

#25071

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Tags

2XMyc

Expression

Mammalian

Mutation

Contains only the kinase domain aa 1863-2218

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
TLS 1: hTLS.pBSKS(+)

Plasmid

#21827

Depositor

David Ron

Article

Crozat et al Nature. 1993 Jun 17;363(6430):640-4.

Insert

TLS (FUS Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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RS314-H

Plasmid

#17541

Depositor

Bert Vogelstein

Article

Thiagalingam et al Proc Natl Acad Sci U S A. 1995 Jun 20. 9

Insert

Gal1-p53 (TP53 Human)

Use

Tags

Expression

Yeast

Mutation

R273H

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pIK127

Plasmid

#65631

Purpose

Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter.

Depositor

Iskra Katic

Article

Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

Insert

Peft-3::gfp::h2b::tbb-2 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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p1.2-Neo

Plasmid

#162739

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CHO Elongation factor 1A

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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AcrIIA2

Plasmid

#101042

Purpose

Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

Depositor

Zhiwei Huang

Article

Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

Insert

AcrIIA2

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TetO-U1-70K-fSNAPc

Plasmid

#106987

Purpose

open reading frame of U1-70K inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

U1-70K (SNRNP70 Human)

Use

Tags

fSNAP

Expression

Mutation

Promoter

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST51-LRRK2-R1441G

Plasmid

#25082

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Destination vector

Tags

6XHis and V5

Expression

Mammalian

Mutation

Arginine 1441 mutated to Glycine

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO_TOMM20-STf

Plasmid

#182002

Purpose

CMV overexpression of SNAP-tag fast at the mitochondria surface of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

TOMM20-STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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OA-1050G (white)

Plasmid

#132420

Purpose

express arrays of gRNA targeting White under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

white gRNA array

Use

Tags

Expression

Insect

Mutation

Promoter

U6-3

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_miniABEmaxNG(GG)

Plasmid

#187062

Purpose

miniABEmax(GG)-SpCas9-NG

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

miniABEmax(GG)-SpCas9-NG

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p1.2-Hygro

Plasmid

#162741

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CHO Elongation factor 1A

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
2XMyc-LRRK2-WD40

Plasmid

#25073

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Tags

2XMyc

Expression

Mammalian

Mutation

Contains only the WD40 domain aa 2155-2527

Promoter

Available since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
pBp-Control Sponge (CXCR4 sponge)

Plasmid

#22744

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only
pGEX-Nedd-4/2522

Plasmid

#11466

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

Nedd4-1 (Nedd4 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 52-777

Promoter

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
pCMV_ABEmaxNG‐NGA

Plasmid

#187055

Purpose

ABEmax-SpCas9-NGA

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

ABEmaxNG‐NGA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNVLTv2-pvdIJD-Ptrc-base

Plasmid

#169243

Purpose

Suicide vector for integrating expression cassette in P. putida (IPTG induction)

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Insert

lacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

lacIq

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pRL-TK-hFluc-CrPV-hRluc-6xbsMS2-Malat_AC

Plasmid

#148443

Purpose

Mammalian Expression of 6xbsMS2-Malat

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-Malat

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH645

Plasmid

#172169

Purpose

Reporter plasmid for pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Pem7 luxCDA(B.1TAG)E; Plpp pylS; PproK pylT

Use

Tags

Expression

Bacterial

Mutation

Inserted amber codon immediately after initiator …

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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YF-Rac1(DN)

Plasmid

#20151

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP-Rac1(DN) (RAC1 Human)

Use

Tags

Expression

Mammalian

Mutation

dominant negative mutation

Promoter

Available since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad33_ISMmu1_reRNA

Plasmid

#205349

Purpose

Bacterial expression of ISMmu1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISMmu1 TnpB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT85_P438-mEOS3.2_GentaR

Plasmid

#173894

Purpose

Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the P438 promoter

Depositor

Yonathan Arfi

Article

Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

Insert

mEos3.2

Use

Tags

Expression

Bacterial

Mutation

Promoter

P438

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-TO-PV-NLS-Dsred

Plasmid

#16341

Depositor

Anton Bennett

Article

Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

Insert

PV-DsRed-NLS (Pvalb Rat)

Use

Tags

Myc and NLS

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
TLS 5:hTLS-CHOP.pCDNA1

Plasmid

#21831

Depositor

David Ron

Article

Crozat et al Nature. 1993 Jun 17;363(6430):640-4.

Insert

TLS-CHOP (FUS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
Erk1

Plasmid

#29578

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

Erk1 (MAPK3 Human)

Use

Tags

FLAG, HIS6, and TEV

Expression

Bacterial

Mutation

K289N mutation present. Starting Met removed from…

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
Flag-p50(1-435)

Plasmid

#44747

Depositor

Gourisankar Ghosh

Article

Savinova et al Mol Cell. 2009 Jun 12;34(5):591-602. doi

Insert

p50 (Nfkb1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

AA 1-435

Promoter

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
OA-1050J (GFP)

Plasmid

#133304

Purpose

express arrays of gRNA targeting GFP under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

GFP gRNA array

Use

Tags

Expression

Insect

Mutation

Promoter

U6-3

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKF-P14MM2AG5h

Plasmid

#128254

Purpose

Mammalian positive feedback synthetic gene circuit within a Flp-In expression vector.

Depositor

Gabor Balazsi

Article

Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

Insert

rtTA-Advanced::P2A::EGFP

Use

Synthetic Biology; Flp-in expression vector

Tags

Expression

Mammalian

Mutation

Promoter

tight TRE promoter

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPTD-DRBD

Plasmid

#35622

Depositor

Steven Dowdy

Article

Eguchi et al Nat Biotechnol. 2009 Jun;27(6):567-71. E

Insert

PKR DRBD-1

Use

Tags

6xHIS, HA, and TAT

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
pBad33_ISAba30_reRNA

Plasmid

#205323

Purpose

Bacterial expression of ISAba30 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISAba30 TnpB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad33_ISDge10_reRNA

Plasmid

#205340

Purpose

Bacterial expression of ISDge10 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISDge10 TnpB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-51b(+) ChemoG2

Plasmid

#193800

Purpose

Protein production of ChemoG2 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG2

Use

Tags

10xHis

Expression

Bacterial

Mutation

EGFP: A206K

Promoter

T7

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-51b(+) ChemoG3

Plasmid

#193801

Purpose

Protein production of ChemoG3 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG3

Use

Tags

10xHis

Expression

Bacterial

Mutation

EGFP:A206K; HT7: L271E

Promoter

T7

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-51b(+) ChemoG 3.2

Plasmid

#193803

Purpose

Protein production of ChemoG3.2 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG 3.2

Use

Tags

10xHis

Expression

Bacterial

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

T7

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-51b(+) ChemoG4

Plasmid

#193804

Purpose

Protein production of ChemoG4 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG4

Use

Tags

10xHis

Expression

Bacterial

Mutation

EGFP:A206K; HT7: E143R, E147R, L271E

Promoter

T7

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMINTC3H

Plasmid

#110075

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and His10 tag.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Tags

3C cleavage site - His10 Tag

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsTNRC6Biso1

Plasmid

#25034

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

TNRC6B (TNRC6B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-nls-NgAgo-GK

Plasmid

#86435

Purpose

Expresses a Flag tag on the N-terminal

Depositor

Gaétan Burgio

Article

Khin et al PLoS One. 2017 Jun 13;12(6):e0178768. do

Insert

NgAgo

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
SPDK3959

Plasmid

#199246

Purpose

Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA vector with AtPDS3 target for editing Arabidopsis PDS3 gene

Depositor

Savithramma Dinesh-Kumar

Article

Nagalakshmi et al Plant Physiol. 2022 Jun 27;189(3):1241-1

Insert

Modified TRV RNA2 with PEBV promoter, AtPDS3 target and AtIleu-tRNA mobile RNA sequence

Use

T-dna vector

Tags

Expression

Mutation

Promoter

PEBV

Available since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMINTC3GH (Hyg)

Plasmid

#110077

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMEXC3GH (Hyg)

Plasmid

#110082

Purpose

Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
2XMyc-LRRK2-LRRs

Plasmid

#25072

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Tags

2XMyc

Expression

Mammalian

Mutation

Contains only the LRR domains aa879-1278

Promoter

Available since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
Lenti-EF1alpha-VP64-dCas9-p300_Blast

Plasmid

#192654

Purpose

3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

VP64-dCas9-p300

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A; H840A in Cas9

Promoter

EF1a

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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